Letter to NeuroscienceThe apical shaft of CA1 pyramidal cells is under GABAergic interneuronal control
Section snippets
Experimental procedures
The surgical and recording methods have been described in detail previously.17 In short, adult Sprague–Dawley rats (200–300 g; Hilltop Laboratories, Scottsdale, PA) were anesthetized with urethane (1.5 g/kg; Sigma, St. Louis, MO, USA) and placed in a stereotaxic apparatus. A small (1.2×1.2 mm) bone window was drilled above the hippocampus (centered at AP=−3.5 and L=2.5 mm from bregma) for intracellular recordings. The micropipettes were filled with 1 M potassium acetate and 1% biocytin (Sigma). In
Acknowledgements
We thank T. F. Freund for his comments, E. Bonder for technical support, K. G. Baimbridge, T. Gorcs, and P. Somogyi for supplying us CB, PV, VIP, CCK and GABA antibodies, respectively. This work was supported by NIH (NS34994, MH54671), MH12403 (D.A.H.), Human Frontier Science Program (X. L.) and Soros Fellowship (E. P).
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2010, Advances in PharmacologyCitation Excerpt :GABABR subunits can also be found in the plasma membrane of dendritic shafts and spines in the vicinity of glutamate/asymmetric synapses (Gonchar et al., 2001; Kulik et al., 2003; Kulik et al., 2006; Lopez-Bendito et al., 2002; Vigot et al., 2006). Although the dendritic membrane of pyramidal neurons (including the spines) may receive some GABA synapses (Andrasfalvy & Mody, 2006; Megias et al., 2001; Papp et al., 2001), GABAB1 subunits in dendritic spines and shafts are commonly found in regions of membrane lacking an inhibitory presynaptic partner (Kulik et al., 2003). This suggests that many of the dendritic GABABRs, which presumably regulate the excitatory effects of glutamate inputs, are extrasynaptic.