Archival ReportVentral Tegmental Area Cannabinoid Type-1 Receptors Control Voluntary Exercise Performance
Section snippets
Animals
All experiments complied with the French and European rules on animal experimentation. This study involved 2-month-old male C57BL/6N mice and 2- to 3-month-old male CB1 receptor mutant and wildtype animals, including constitutive CB1 receptor mutant mice (CB1–/–), conditional mutant mice lacking CB1 receptors from principal neurons (CaMK-CB1–/–), brain GABAergic neurons (GABA-CB1–/–), cortical glutamatergic neurons (Glu-CB1–/–), serotonergic neurons (TPH2-CB1–/–), dopamine D1
CB1 Receptors on Brain GABAergic Neurons Control Voluntary Running
Mutant mice lacking CB1 receptors from the whole body (CB1–/–) and their wildtype littermates (CB1+/+), bred as in Dubreucq et al. (14), were offered 3-hour daily access to running wheels for 2 weeks. This paradigm allowed observation of plateau levels of performance in both genotypes (Figure 1A). CB1–/– mice displayed low running activity, compared with CB1+/+ mice [F(1,35) = 8.22, p = .007], the amplitude of this difference increasing with the number of running sessions [F(13,455) = 3.23, p =
Discussion
Mutant mice lacking CB1 receptors have been reported to spend less time running and to run shorter distances than their wildtype littermates when housed with running wheels 14, 15. In these studies, however, the animals had unlimited access to the wheels, thus questioning the role of CB1 receptors during shorter running activities. To solve this issue, we used a paradigm in which mice had daily 3-hour access to exercise. This exercise duration was selected because it is sufficient to observe
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2021, NeuroscienceCitation Excerpt :Consistently, intraperitoneal injection of CB1R antagonist SR141716 decreases the running distance and speed in a dose-dependent manner in mice (Keeney et al., 2008). Further studies identified that CB1R located on GABAergic terminals in the ventral tegmental area exerts a crucial control on mouse wheel-running behavior (Dubreucq et al., 2013). In addition, previous study proposed that endocannabinoids stimulate movement through CB1R-dependent excitability regulation and short-/long-term synaptic plasticity in the basal ganglia and cortex (El Manira and Kyriakatos, 2010).
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2021, PsychoneuroendocrinologyCitation Excerpt :These data indicate that repeated stimulation of the eCB system may have long-term consequences on eCB levels that have insufficiently been studied in humans yet. It was also shown that CB1 receptors control rodent voluntary running performance in VTA GABAergic terminals (Dubreucq et al., 2013), highlighting a potential role for eCBs in motivational aspects of regular endurance exercise. We performed a urine drug screen with all participants on both days of testing, ruling out prior recreational cannabis use since we were concerned that cannabis use might interfere with the eCB system.
Authors GM, FG, and FC contributed equally to this work