Assessment of the ameliorative role of selenium nanoparticles on the oxidative stress of acetaminophen in some tissues of male albino rats

Abstract

Our present study aimed to assess the ameliorative role of selenium nanoparticles (Se-NPs) with diameters 3–5 nm and 10–20 nm (Detected by Transmission Electron microscopy), as an antioxidant against acetaminophen toxicity in the brain and kidney of male albino rats. The experimental rats were divided into four groups, each with eight rats. The group I were intraperitoneally injected with saline, the group II were orally administrated paracetamol (500 mg/kg b. w.) for two successive days, whereas rats of the groups III and VI were intraperitoneally injected with 0.5 mg Se-NPs of diameters 3–5 nm and 10–20 nm respectively, two days before paracetamol administration and continued with its administration. The brain and renal oxidative stresses were assessed by increased tissue malondialdehyde, nitric oxide concentration, DNA fragmented, and significant decrease of glutathione content and total antioxidant capacity. The brain gamma-glutamyltransferase (γ-GT) and butyryl cholinesterase (BCHE) were also increased. On the other hand, treatment of rats with selenium nanoparticles with two different diameters 3–5 nm and 10–20 nm respectively reversed paracetamol-related toxic via more than one mechanism such as the powerful inhibition of malondialdehyde and nitric oxide concentrations in previous tissue. Stimulation of the synthesis of cellular antioxidants represented by significant increase of GSH concentration and significant decrease of the inflammatory response represented by TNF-α concentration. Also DNA fragmentation % significantly decreased in the same tissues. The present results concluded that paracetamol overdose induced oxidative damage in brain and renal tissues were improved by selenium nanoparticles with two different diameters with its antioxidant effects.