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Detection of infectious endometritis is challenging.
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No single diagnostic test is capable of detecting all cases of infectious endometritis.
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A guarded swab and cytology brush can be used as initial screening diagnostics to be analyzed by microbial culture and cytologic evaluation diagnosis of infectious endometritis.
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If results from the initial screening testing are negative for mares with clinical signs suggestive of infectious endometritis, additional samples collected by small-volume lavage or
Veterinary Clinics of North America: Equine Practice
Endometritis: Diagnostic Tools for Infectious Endometritis
Section snippets
Key points
Summary
Detection of infectious endometritis, while challenging in certain cases, usually can be easily diagnosed or ruled out using a sample from a guarded swab and cytology brush, small volume lavage, or uterine biopsy. These samples are routinely submitted for microbial culture and cytologic evaluation. However, there is not a gold standard diagnostic sample or analysis of the sample that is quick to perform, is inexpensive, and returns rapid results.
In a clinical setting, an option for screening
References (45)
- et al.
Comparison of three diagnostic methods to identify subclinical endometritis in mares
Theriogenology
(2011) - et al.
Importance of using guarded techniques for the preparation of endometrial cytology smears in mares
Theriogenology
(2006) Endometritis in the mare: a diagnostic study comparing cultures from swab and biopsy
Theriogenology
(2005)- et al.
Relationships between uterine culture, cytology and pregnancy rates in a Thoroughbred practice
Theriogenology
(2007) - et al.
Diagnosis of endometritis in the mare based on bacteriological and cytological examinations of the endometrium: comparison of results obtained by swabs and biopsies
J Equine Vet Sci
(2010) - et al.
Use of a low-volume uterine flush for microbiologic and cytologic examination of the mare's endometrium
Theriogenology
(1988) - et al.
Use of a low-volume uterine flush for diagnosing endometritis in chronically infertile mares
Theriogenology
(2007) - et al.
Comparison of the cytobrush, cottonswab, and low volume uterine flush techniques to evaluate endometrial cytology for diagnosing endometritis in chronically infertile mares
Theriogenology
(2012) - et al.
Equine endometrial cytology
Vet Clin N Am-equine Pract
(1988) - et al.
Technique and interpretation of cervical and endometrial cytology in the mare
J Equine Vet Sci
(1984)
Post-breeding inflammation and endometrial cytology in mares
Theriogenology
Molecular diagnostic methods for invasive fungal disease: the horizon draws nearer?
Pathology
Use of a qualitative horse-side test to measure serum amyloid a in mares with experimentally induced ascending placentitis
J Equine Vet Sci
Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis
Vet Immunol Immunopathol
The evaluation of biofilm-forming potential of Escherichia coli collected from the equine female reproductive tract
J Equine Vet Sci
Biofilms and their relevance to veterinary medicine
Vet Microbiol
Medical problems of adult horses, as ranked by equine practitioners
J Am Vet Med Assoc
Clinical and subclinical endometritis in the mare: both threats to fertility
Reprod Domest Anim
Endometrial microbial isolates are associated with different ultrasonographic and endometrial cytology findings in Thoroughbred mares
Proc Am Assoc Eq Pract
Comparison of equine endometrial cytology samples collected with uterine swab, uterine brush, and low-volume lavage from healthy mares
Vet Clin Pathol
Comparison of three methods of sampling for endometrial cytology in the mare
Tierarztl Prax Ausg G Grosstiere Nutztiere
Results of concurrent bacteriological and cytological examinations of the endometrium of mares in routine stud farm practice 1978-1981
J Reprod Fertil Suppl
Cited by (22)
Success of different therapies for bacterial endometritis in stud farm practice
2024, Journal of Equine Veterinary ScienceComparison Between Gynecological Examination Methods and Sample Collection Techniques for the Diagnosis of Endometritis in Subfertile Mares
2022, Journal of Equine Veterinary ScienceCitation Excerpt :Regardless, each technique has advantages and disadvantages in practicality, costs, sampling locations of the uterus, quantity, and quality of the collected sample. In this context, there is still no single method that comprehends ease in execution, low cost, and safe results for an accurate diagnosis [12]. Several studies have tested exam methods and sample collection techniques [1,8,9,13–18], however, there is still no standardization of methodologies used for diagnosis.
Uterine Inflammatory Response After Prostaglandin E1 (Misoprostol) Infusion Prebreeding or Immediately After Embryo Flushing in Commercial Donor Mares
2022, Journal of Equine Veterinary ScienceBacteria Isolated From Equine Uteri in The United Arab Emirates: A Retrospective Study
2022, Journal of Equine Veterinary ScienceCitation Excerpt :All these bacteria featured in the list of those isolated from the samples in this study, albeit in some cases representing <1% of bacterial isolates. Ferris [3] suggested that these bacteria may have questionable pathogenicity and more recent work has also shown that Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria are part of the normal equine endometrial microbiome [19,20]. Staphylococcus aureus, a Gram-positive bacteria, which frequently features in lists of bacterial isolates from uterine cultures was present in <1% of the isolates in the present study.
Infectious Endometritis in Mares: Microbiological Findings in Field Samples. <sup>☆</sup>
2022, Journal of Equine Veterinary ScienceCitation Excerpt :This finding underlines the importance of the enrichment step, regardless of the pathogenic significance of every bacteria species that was attributed by the practitioner on the basis of the mare's clinical history. Indeed, a positive culture should be considered diagnostic of infectious endometritis as these results are associated with reduced pregnancy rates [9,20,28,37]. Little is known about equine vaginal microbiota.
The author has nothing to disclose.