Elsevier

Food Microbiology

Volume 60, December 2016, Pages 137-141
Food Microbiology

A peptidome-based phylogeny pipeline reveals differential peptides at the strain level within Bifidobacterium animalis subsp. lactis

https://doi.org/10.1016/j.fm.2016.06.015Get rights and content

Highlights

  • Some B. lactis strains are considered probiotics, as they exert beneficial effects on the human host.

  • Current strains used in food nutrition show limited genetic variability, making difficult to differentiate among strains.

  • Our peptidome-based analysis using whole proteomes to obtain peptidomes simulating gut endopeptidases in silico.

  • This pipeline allowed detecting strain-specific peptides.

  • Differential peptides may have applications for detecting specific strains in functional foods or in the gut microbiota.

Abstract

Bifidobacteria are gut commensal microorganisms belonging to the Actinobacteria group. Some specific strains of Bifidobacterium animalis subsp. lactis are used in functional foods as they are able to exert health-promoting effects in the human host. Due to the limited genetic variability within this subspecies, it is sometimes difficult for a manufacturer to properly track its strain once included in dairy products or functional foods. In this paper, we present a peptidome-based analysis in which the proteomes of a set of B. animalis subsp. lactis strains were digested in silico with human gut endopeptidases. The molecular masses were compared along all the strains to detect strain-specific peptides. These peptides may be interesting towards the development of methodologies for strain identification in the final product.

Section snippets

Acknowledgements

This research was funded by Grant AGL2013-44039-R from the Spanish “Plan Estatal de I+D+I”, and by Grant EM2014/046 from the “Plan Galego de investigación, innovación e crecemento 2011–2015”. Borja Sánchez was recipient of a Ramón y Cajal postdoctoral contract from the Spanish Ministry of Economy and Competitiveness (RYC-2012-10052).

This work was also partially funded by the [14VI05] Contract-Programme from the Unixikversity of Vigo and the Agrupamento INBIOMED from DXPCTSUG-FEDER unha maneira

References (25)

  • O. Horlacher et al.

    MzJava: an open source library for mass spectrometry data processing

    J. Proteomics

    (2015)
  • J.P. Huelsenbeck et al.

    MRBAYES: Bayesian inference of phylogenetic trees

    Bioinformatics

    (2001)
  • Cited by (0)

    View full text