Elsevier

Journal of Biotechnology

Volume 251, 10 June 2017, Pages 53-58
Journal of Biotechnology

Short communication
Insights from the genome of a high alkaline cellulase producing Aspergillus fumigatus strain obtained from Peruvian Amazon rainforest

https://doi.org/10.1016/j.jbiotec.2017.04.010Get rights and content

Highlights

  • First whole genome sequence based report of a high cellulase producing non-clinical Aspergillus fumigatus strain LMB-35Aa isolated from forest soil.

  • This strain showed consistently higher activity in a wide range of pH which could be highly beneficial in future for industrial purpose.

  • Illumina paired end technology was used to sequence the whole genome.

  • The genome is ∼27.5 mb long and comprises of 228 scaffolds with 50% average GC content.

  • A sum of 8660 protein-coding genes was predicted in A. fumigatus LMB-35Aa strain among which 6156 genes were found with known function.

Abstract

Here, we report the complete genome sequence of a high alkaline cellulase producing Aspergillus fumigatus strain LMB-35Aa isolated from soil of Peruvian Amazon rainforest. The genome is ∼27.5 mb in size, comprises of 228 scaffolds with an average GC content of 50%, and is predicted to contain a total of 8660 protein-coding genes. Of which, 6156 are with known function; it codes for 607 putative CAZymes families potentially involved in carbohydrate metabolism. Several important cellulose degrading genes, such as endoglucanase A, endoglucanase B, endoglucanase D and beta-glucosidase, are also identified. The genome of A. fumigatus strain LMB-35Aa represents the first whole sequenced genome of non-clinical, high cellulase producing A. fumigatus strain isolated from forest soil.

Section snippets

Data deposition

The genome version discussed in this paper is from Aspergillus fumigatus strain LMB-35Aa. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession MCQI00000000. The version described in this paper is version MCQI02000000 (MCQI00000000.2).

Competing interests

The authors declare that they have no competing interests.

Acknowledgements

This work was supported by grants from Concejo Nacional de Ciencia y Tecnología of Peru [N° 002-2013-CONCYTEC-FONDECYT and N° 181-2015-FONDECYT-DE] and Programa Nacional de Innovación para la Competitividad y Productividad, Ministry of Production of Peru [N° 176-FINCyT-IB-2013 and N° 177-FINCyT-IB-2013]. This work was also supported by the National Science Foundation under the CCI Center for Selective C−H Functionalization (CHE-1205646), and the Hans W. Vahlteich Professorship (D.H.S.). We

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