Distribution, virulence-associated genes and antimicrobial resistance of Aeromonas isolates from diarrheal patients and water, China
Graphical abstract
Section snippets
Active surveillance of Aeromonas
Stool specimens were collected from diarrheal patients admitted to sixteen hospitals in the city of Shanghai, China, from June 25, 2010 to Dec. 31, 2012. Patients age from 5 month to 82 years (mean age 42.1 ± 19.2). Specimens were collected by rectal swabs in Cary–Blair transport media and then transported to the laboratories of the Pudong District Center for Disease Prevention and Control (CDC), Shanghai, and the Shanghai Public Health Clinical Center, Fudan University. For the isolation of
Prevalence of Aeromonas and co-infection of active surveillance
We collected 5069 specimens in our active surveillance, including 4529 stool specimens from diarrheal patients and 540 aquatic samples. By traditional biochemical tests: oxidase positive, have facultative anaerobic metabolism, resistance to O/129 (150 μg) (Oxoid), and API-20E system (BioMérieux, France), 193 (4.3%) samples from diarrheal patients and 64 (11.9%) samples from the aquatic environment were identified as the genus level of Aeromonas strains.
For diarrheal patients, Aeromonas was
Discussion
Our active surveillance indicated the wide-spread distribution of Aeromonas species in aquatic environments, which were the major sources of contamination in acquired Aeromonas infections.1 The percentage of Aeromonas-positive samples from diarrheal patients was 4.5%, which is similar to the rate in other countries.3, 18 Here, we did not find proof of outbreaks caused by Aeromonas alone, so the role of Aeromonas as an etiologic agent of diarrhea is still to be a controversial issue,19 further
Acknowledgments
This work was supported by the Priority Project on Infectious Disease Control and Prevention (2008ZX10004-008, 201202006, 2012ZX10004215, 2012ZX10004-201 and 2013ZX10004221-004) from the Ministry of Health, China, Key discipline construction of health system in Pudong New Area, Shanghai, China (No. PWZxk2010-09).
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