Elsevier

Molecular Metabolism

Volume 12, June 2018, Pages 98-106
Molecular Metabolism

Brief Communication
mTORC1-dependent increase in oxidative metabolism in POMC neurons regulates food intake and action of leptin

https://doi.org/10.1016/j.molmet.2018.04.002Get rights and content
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open access

Highlights

  • H2O2 requires mTORC1 activity to increase oxidant levels in POMC neurons.

  • H2O2 requires functional mTORC1 in POMC neurons to inhibit food intake.

  • Leptin increases ROS-related signal in POMC neurons by engaging mTORC1.

  • Leptin requires functional mTORC1 in POMC neurons to inhibit food intake.

Abstract

Objective

Nutrient availability modulates reactive oxygen species (ROS) production in the hypothalamus. In turn, ROS regulate hypothalamic neuronal activity and feeding behavior. The mechanistic target of rapamycin complex 1 (mTORC1) pathway is an important cellular integrator of the action of nutrients and hormones. Here we tested the hypothesis that modulation of mTORC1 activity, particularly in Proopiomelanocortin (POMC)-expressing neurons, mediates the cellular and behavioral effects of ROS.

Methods

C57BL/6J mice or controls and their knockout (KO) littermates deficient either for the mTORC1 downstream target 70-kDa ribosomal protein S6 kinase 1 (S6K1) or for the mTORC1 component Rptor specifically in POMC neurons (POMC-rptor-KO) were treated with an intracerebroventricular (icv) injection of the ROS hydrogen peroxide (H2O2) or the ROS scavenger honokiol, alone or, respectively, in combination with the mTORC1 inhibitor rapamycin or the mTORC1 activator leptin. Oxidant-related signal in POMC neurons was assessed using dihydroethidium (DHE) fluorescence.

Results

Icv administration of H2O2 decreased food intake, while co-administration of rapamycin, whole-body deletion of S6K1, or deletion of rptor in POMC neurons impeded the anorectic action of H2O2. H2O2 also increased oxidant levels in POMC neurons, an effect that hinged on functional mTORC1 in these neurons. Finally, scavenging ROS prevented the hypophagic action of leptin, which in turn required mTORC1 to increase oxidant levels in POMC neurons and to inhibit food intake.

Conclusions

Our results demonstrate that ROS and leptin require mTORC1 pathway activity in POMC neurons to increase oxidant levels in POMC neurons and consequently decrease food intake.

Keywords

Hypothalamus
Reactive oxygen species
mTORC1
Leptin
Food intake
POMC

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