The fluoride coated AZ31B magnesium alloy improves corrosion resistance and stimulates bone formation in rabbit model

https://doi.org/10.1016/j.msec.2016.03.016Get rights and content

Highlights

  • Fluoride coating inhibited the degradation of the alloy in the early implantation.

  • Fluorine coating could slow down the rate of Mg corrosion and Mg ion release.

  • Fluorine coating could promote the deposition of Ca and P in vivo.

  • Fluorine coated Mg alloy had well osteogenic activity and biocompatibility.

  • Fluorine coating up-regulated the expression of BMP-2 and collagen type I protein.

Abstract

This study aimed to evaluate the effect of fluorine coated Mg alloy and clarify its mechanism in bone formation. We implanted the fluorine coated AZ31B Mg alloy screw (group F) in rabbit mandibular and femur in vivo. Untreated AZ31B Mg alloy screw (group A) and titanium screw (group T) were used as control. Then, scanning electron microscopy, the spectral energy distribution analysis, hard and decalcified bone tissues staining were performed. Immunohistochemistry was employed to examine the protein expressions of bone morphogenetic protein 2 (BMP-2) and collagen type I in the vicinity of the implant. Compared with the group A, the degradation of the alloy was reduced, the rates of Mg corrosion and Mg ion release were slowed down, and the depositions of calcium and phosphate increased in the group F in the early stage of implantation. Histological results showed that fluorine coated Mg alloy had well osteogenic activity and biocompatibility. Moreover, fluoride coating obviously up-regulated the expressions of collagen type I and BMP-2. This study confirmed that the fluorine coating might improve the corrosion resistance of AZ31B Mg alloy and promote bone formation by up-regulated the expressions of collagen type I and BMP-2.

Abbreviations

BMP-2
bone morphogenetic protein 2
Mg
magnesium
BMPs
bone morphogenetic protein
SEM
scanning electron microscopy
SED
spectral energy distribution
HE
hematoxylin-eosin
MOD
mean optical density

Keywords

Fluorine coated AZ31B magnesium alloy
Osteogenic activity
Corrosion resistance
Bone morphogenetic protein 2
Collagen type I

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