Dynamics and organization of proteins in the neuronal plasma membrane

doi:10.1016/j.neuropharm.2020.108043

Neuropharmacology

Volume 169, 1 June 2020, 108043

https://doi.org/10.1016/j.neuropharm.2020.108043 Get rights and content

References (14)

A. Barberis
Postsynaptic plasticity of GABAergic synapses
Neuropharmacology
(2020)
I. Chamma et al.
Biophysical mechanisms underlying the membrane trafficking of synaptic adhesion molecules
Neuropharmacology
(2020)
E. Come et al.
KCC2 membrane diffusion tunes neuronal chloride homeostasis
Neuropharmacology
(2020)
J.P. Dupuis et al.
Surface trafficking of neurotransmitter receptors: from cultured neurons to intact brain preparations
Neuropharmacology
(2020)
S. Hannan et al.
GABAAR isoform and subunit structural motifs determine synaptic and extrasynaptic receptor localisation
Neuropharmacology
(2020)
M. Heine et al.
Dynamic compartmentalization of calcium channel signalling in neurons
Neuropharmacology
(2020)
J.B. Masson et al.
Mapping the energy and diffusion landscapes of membrane proteins at the cell surface using high-density single-molecule imaging and Bayesian inference: application to the multiscale dynamics of glycine receptors in the neuronal membrane
Biophys. J.
(2014)

There are more references available in the full text version of this article.

Cited by (3)

Conformational state-dependent regulation of GABA<inf>A</inf> receptor diffusion and subsynaptic domains
2022, iScience
Citation Excerpt :
Interaction of the GABAAR with its main scaffolding protein, gephyrin, is responsible for its confinement and synaptic aggregation (Jacob et al., 2005; Mukherjee et al., 2011; Petrini et al., 2014; Renner et al., 2012). Regulation of receptor lateral diffusion is considered the first mechanism for adapting the number of receptors at synapses in response to synaptic demand (Choquet and Triller, 2013; Triller and Choquet, 2008; Triller et al., 2020). The lateral diffusion of GABAARs is regulated by neuronal activity (Bannai et al., 2009,2015; Muir et al., 2010; Petrini and Barberis, 2014; Petrini et al., 2014), providing the molecular basis underlying synaptic plasticity at inhibitory GABAergic synapses of the hippocampus (Petrini and Barberis, 2014; Petrini et al., 2014).
The efficacy of GABAergic synapses relies on the number of postsynaptic GABA_A receptors (GABA_ARs), which is regulated by a diffusion capture mechanism. Here, we report that the conformational state of GABA_ARs influences their membrane dynamics. Indeed, pharmacological and mutational manipulations of receptor favoring active or desensitized states altered GABA_AR diffusion leading to the disorganization of GABA_AR subsynaptic domains and gephyrin scaffold, as detected by super-resolution microscopy. Active and desensitized receptors were confined to perisynaptic endocytic zones, and some of them were further internalized. We propose that following their activation or desensitization, synaptic receptors rapidly diffuse at the periphery of the synapse where they remain confined until they switch back to a resting state or are internalized. We speculate that this allows a renewal of activatable receptors at the synapse, contributing to maintain the efficacy of the synaptic transmission, in particular on sustained GABA transmission.
The distribution of the iron oxide nanoparticles modified with polyethylene glycol in rat brains
2021, Materials Chemistry and Physics
Citation Excerpt :
The interaction between nanoparticles and cells determine the fate and distribution of the nanoparticles, the latter is closely related to hyperthermia, magnetic resonance imaging, and drug delivery [2–7]. Studies have shown that there are many receptors and channels on the cell membrane of neurons (TRPV1, etc.) [8–10]. The external magnetic field could be used to act on these channels through magnetic nanoparticles targeting to the cell membranes for remote control of neuron excitation [8,11,12], the TRPV1 channel can be activated by warming it (>43 °C) or by a certain magnetic moment tension [12].
The distribution characteristics of nanoparticles associated with cells are crucial to many applications, including magnetic hyperthermia, drug carriers, etc. To clarify the locations when nanoparticles enter the in vivo environments is a necessity. In this work, superparamagnetic iron oxide nanoparticles modified with polyethylene glycol (PEG-SPIONs) were prepared by high temperature thermal decomposition method, and then stereotaxic injections of PEG-SPIONs into the substantia nigra of Sprague-Dawley rats were performed. The distribution of nanoparticles in rat brain was observed by inductively coupled plasma emission spectrometer (ICP-OES) and transmission electron microscopy (TEM). It was found that the iron contents were higher in the olfactory bulb, temporal lobe, prefrontal cortex and thalamus after the injection of PEG-SPIONs for 24 h and 7 days, PEG-SPIONs were mostly distributed around the axonal membrane and dendritic membrane, and a small amount were distributed near the myelin membrane and mitochondrial membrane, which can be ascribed to the nature of PEG, the hydration size and Zeta potential of PEG-SPIONs. Along with their magnetocaloric effect the PEG-SPIONs have potential to act on the ion channels on the cell membrane under an alternating current magnetic field.
Stochastic self-assembly of reaction-diffusion patterns in synaptic membranes
2021, Physical Review E

View full text

EditorialDynamics and organization of proteins in the neuronal plasma membrane

Neuropharmacology

Neuropharmacology

Neuropharmacology

Neuropharmacology

Neuropharmacology

Neuropharmacology

Biophys. J.

Editorial
Dynamics and organization of proteins in the neuronal plasma membrane