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Putative markers of infective life stages in Leishmania (Viannia) braziliensis

Published online by Cambridge University Press:  26 September 2007

D. GAMBOA ,
G. VAN EYS ,
K. VICTOIR ,
K. TORRES ,
V. ADAUI ,
J. AREVALO  and
J.-C. DUJARDIN
D. GAMBOA
Affiliation:
Instituto de Medicina Tropical ‘Alexander von Humboldt’, Universidad Peruana Cayetano Heredia, A.P. 4314, Lima 100, Peru
G. VAN EYS
Affiliation:
Department of Molecular Genetics, University of Maastricht, P.O. Box 616, 6200 MD, Maastricht, The Netherlands
K. VICTOIR
Affiliation:
Unit of Molecular Parasitology, Intituut voor Tropische Geneeskunde, 155 Nationalestraat, B-2000 Antwerpen, Belgium
K. TORRES
Affiliation:
Instituto de Medicina Tropical ‘Alexander von Humboldt’, Universidad Peruana Cayetano Heredia, A.P. 4314, Lima 100, Peru
V. ADAUI
Affiliation:
Instituto de Medicina Tropical ‘Alexander von Humboldt’, Universidad Peruana Cayetano Heredia, A.P. 4314, Lima 100, Peru
J. AREVALO
Affiliation:
Instituto de Medicina Tropical ‘Alexander von Humboldt’, Universidad Peruana Cayetano Heredia, A.P. 4314, Lima 100, Peru Departamento de Bioquimica, Biologia Molecular y Farmacologia, Facultad de Ciencias y Filosofia, Universidad Peruana Cayetano Heredia, A.P. 4314, Lima 100, Peru
J.-C. DUJARDIN*
Affiliation:
Unit of Molecular Parasitology, Intituut voor Tropische Geneeskunde, 155 Nationalestraat, B-2000 Antwerpen, Belgium
*
*Corresponding author: Unit of Molecular Parasitology, Intituut voor Tropische Geneeskunde, 155 Nationalestraat, B-2000 Antwerpen, Belgium. Tel: 32 3 2476355. Fax: 32 3 2476359. E-mail: jcdujard@itg.be
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Summary

Gene expression is known to vary significantly during the Leishmania life-cycle. Its monitoring might allow identification of molecular changes associated with the infective stages (metacyclics and amastigotes) and contribute to the understanding of the complex host-parasite relationships. So far, very few studies have been done on Leishmania (Viannia) braziliensis, one of the most pathogenic species. Such studies require, first of all, reference molecular markers. In the present work, we applied differential display analysis (DD analysis) in order to identify transcripts that might be (i) candidate markers of metacyclics and intracellular amastigotes of L. (V.) braziliensis or (ii) potential controls, i.e. constitutively expressed. In total, 48 DNA fragments gave reliable sequencing data, 29 of them being potential markers of infective stages and 12 potential controls. Eight sequences could be identified with reported genes. Validation of the results of DD analysis was done for 4 genes (2 differentially expressed and 2 controls) by quantitative real-time PCR. The infective insect stage-specific protein (meta 1) was more expressed in metacyclic-enriched preparations. The oligopeptidase b showed a higher expression in amastigotes. Two genes, glucose-6-phosphate dehydrogenase and a serine/threonine protein kinase, were found to be similarly expressed in the different biological samples.

Keywords

Leishmania (Viannia) braziliensisdifferential displaygene expression
Type
Research Article
Information
Parasitology , Volume 134 , Issue 12 , November 2007 , pp. 1689 - 1698
Copyright
Copyright © Cambridge University Press 2007

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