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Sarcocystis masoni, n. sp. (Apicomplexa: Sarcocystidae), and redescription of Sarcocystis aucheniae from llama (Lama glama), guanaco (Lama guanicoe) and alpaca (Vicugna pacos)

Published online by Cambridge University Press:  02 March 2016

GASTÓN MORÉ ,
CRISTIAN REGENSBURGER ,
M. LAURA GOS ,
LAIS PARDINI ,
SHIV K. VERMA ,
JULIANA CTIBOR ,
MARCOS ENRIQUE SERRANO-MARTÍNEZ ,
JITENDER P. DUBEY  and
M. CECILIA VENTURINI
GASTÓN MORÉ*
Affiliation:
Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
CRISTIAN REGENSBURGER
Affiliation:
Unidad Académica Caleta Olivia, Universidad Nacional de la Patagonia Austral, Santa Cruz, Argentina
M. LAURA GOS
Affiliation:
Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
LAIS PARDINI
Affiliation:
Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
SHIV K. VERMA
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705-2350, USA
JULIANA CTIBOR
Affiliation:
Unidad Académica Caleta Olivia, Universidad Nacional de la Patagonia Austral, Santa Cruz, Argentina
MARCOS ENRIQUE SERRANO-MARTÍNEZ
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Universidad Peruana Cayetano Heredia, Lima, Perú
JITENDER P. DUBEY
Affiliation:
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, Maryland 20705-2350, USA
M. CECILIA VENTURINI
Affiliation:
Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, La Plata, Argentina
*
*Corresponding author. Universidad Nacional de La Plata, Facultad de Ciencias Veterinarias, Calle 60 y 118 (Zip code: 1900) La Plata, Argentina. Tel: +54 221 4249621. Fax: +54 221 4257980. E-mail: gastonmore@fcv.unlp.edu.ar
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Summary

There is considerable confusion concerning the species of Sarcocystis in South American camelids (SAC). Several species names have been used; however, proper descriptions are lacking. In the present paper, we redescribe the macroscopic sarcocyst forming Sarcocystis aucheniae and describe and propose a new name, Sarcocystis masoni for the microscopic sarcocyst forming species. Muscles samples were obtained from llamas (Lama glama) and guanacos (Lama guanicoe) from Argentina and from alpacas (Vicugna pacos) and llamas from Peru. Individual sarcocysts were processed by optical and electron microscopy, and molecular studies. Microscopic sarcocysts of S. masoni were up to 800 µm long and 35–95 µm wide, the sarcocyst wall was 2·5–3·5 µm thick, and had conical to cylindrical villar protrusions (vp) with several microtubules. Each vp had 11 or more rows of knob-like projections. Seven 18S rRNA gene sequences obtained from sarcocysts revealed 95–96% identity with other Sarcocystis spp. sequences reported in the GenBank. Sarcocysts of S. aucheniae were macroscopic, up to 1·2 cm long and surrounded by a dense and laminar 50 µm thick secondary cyst wall. The sarcocyst wall was up to 10 µm thick, and had branched vp, appearing like cauliflower. Comparison of the 11 sequences obtained from individual macroscopic cysts evidenced a 98–99% of sequence homology with other S. aucheniae sequences. In conclusion, 2 morphologically and molecularly different Sarcocystis species, S. masoni (microscopic cysts) and S. aucheniae (macroscopic cysts), were identified affecting different SAC from Argentina and Peru.

Keywords

Sarcocystis spp. South American camelidsPCRsequencingelectron microscopyArgentinaPeru
Type
Research Article
Information
Parasitology , Volume 143 , Issue 5 , April 2016 , pp. 617 - 626
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Copyright
Copyright © Cambridge University Press 2016 

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