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Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae)

Published online by Cambridge University Press:  25 May 2016

Matheus Pereira dos Santos ,
George Shigueki Yasui ,
Pedro Luiz Porfírio Xavier ,
Nadya Soares de Macedo Adamov ,
Nivaldo Ferreira do Nascimento ,
Takafumi Fujimoto ,
José Augusto Senhorini  and
Laura Satiko Okada Nakaghi
Matheus Pereira dos Santos*
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14884–900, Brazil.
George Shigueki Yasui
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemesio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil.
Pedro Luiz Porfírio Xavier
Affiliation:
Department of Veterinary Medicine–FZEA, University of São Paulo, Avenida Duque de Caxias Norte 225, Pirassununga, SP 13630–080, Brazil.
Nadya Soares de Macedo Adamov
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemesio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil.
Nivaldo Ferreira do Nascimento
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14884–900, Brazil.
Takafumi Fujimoto
Affiliation:
Faculty of Fisheries Sciences, Hokkaido University, 3–1-1 Minato-cho, 041–8611, Hakodate, Japan.
José Augusto Senhorini
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemesio Pereira de Godoy, Pirassununga, SP 13630–970, Brazil.
Laura Satiko Okada Nakaghi
Affiliation:
Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14884–900, Brazil.
*
All correspondence to: Matheus Pereira dos Santos, Aquaculture Center, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, SP 14884–900, Brazil. E-mail: matheuspereira@zootecnista.com.br
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Summary

The aim of this study was to describe the morphology of gametes, post-fertilization events and subsequent temperature effects on the early developmental stages of the neotropical species Astyanax altiparanae. The sperm of this species presents a typical morphology of teleost sperm with a spherical head (diameter = 1.88 µm), midpiece (diameter = 0.75 µm) and a single flagellum (length = 18.67 µm). The extrusion of the second polar body and fusion of male and female pronucleus were reported for the first time in this species. Additionally, we observed the formation of the fertilization cone, which prevents polyspermic fertilization. Developmental stages at 22°C, 26°C and 30°C gave rise to fertilization rates at 91.12, 91.42 and 93.04% respectively. Hatching occurred at 25 hpf at 22°C, 16 hpf at 26°C and 11 hpf at 30°C and the hatching rates were 61.78%, 62.90% and 59.45%, respectively. At 22°C, the second polar body was extruded at ≈6 mpf and the male and female pronucleus fused at ≈10 mpf. This fundamental information is important for the field and opens up new possibilities in fish biotechnology, including micromanipulation and chromosome-set manipulation.

Keywords

EmbryoFishOocyteReproductionSpermatozoa
Type
Research Article
Information
Zygote , Volume 24 , Issue 6 , December 2016 , pp. 795 - 807
Copyright
Copyright © Cambridge University Press 2016 

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