Photosynthetica 2001, 39(4):507-513 | DOI: 10.1023/A:1015691626107

Changes in Chlorophyll a Fluorescence, Lipid Peroxidation, and Detoxificant System in Potato Plants Grown under Filtered and Non-Filtered Air in Open-Top Chambers

A. Calatayud, J.W. Alvarado, E. Barreno

Its high oxidant capacity and ability to generate reactive oxygen species cause ozone toxicity. We studied the effect of ambient ozone on chlorophyll (Chl) a fluorescence, antioxidant enzymes, ascorbate contents, and lipid peroxidation in potatoes grown in open-top chambers in the field. In plants grown in non-filtered air (NFA), the development of non-photochemical quenching brought about a decrease in photosystem 2 (PS2) photochemical efficiency. Also the ability of PS2 to reduce the primary acceptor QA was lower than in charcoal-filtered, ozone-free air (CFA). Changes in Chl fluorescence yield were associated with changes in the thylakoid membrane. Ozone altered chloroplast membrane properties, as indicated by an increase in membrane lipid peroxidation in FNA-leaves compared to CFA plants. The ascorbate pool and activities of antioxidant enzymes were used for an indication of the detoxification system state in NFA and CFA leaves, whereby ozone affects the ascorbate concentration and decreases the antioxidant enzymes activities. The capacity of both detoxifying systems together was not high enough to protect potato plants against ambient ozone concentrations which reduced the photosynthetic yield in this potato cultivar.

Additional key words: antioxidant enzymes; ascorbate; lipid peroxidation; non-photochemical quenching; ozone; photochemical quenching; Solanum tuberosum

Published: December 1, 2001  Show citation

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Calatayud, A., Alvarado, J.W., & Barreno, E. (2001). Changes in Chlorophyll a Fluorescence, Lipid Peroxidation, and Detoxificant System in Potato Plants Grown under Filtered and Non-Filtered Air in Open-Top Chambers. Photosynthetica, 39(4), 507-513. doi: 10.1023/A:1015691626107
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