Abstract
Potato yellow vein virus (PYVV) is a whitefly-transmitted (Trialeurodes vaporariorum) closterovirus (WTC) with an as yet unidentified genome composition. PYVV dsRNA preparations consist of three high molecular weight dsRNA species (dsRNAs 1, 2 and 3) 8.0, 5.5 and 4.0 kbp in size respectively, as well as two low molecular weight dsRNA species of 2.0 and 1.8 kbp (denoted x and y). The PYVV capsid protein minor (CPm) gene was identified on the dsRNA 3 species, and was subsequently cloned and sequenced. The PYVV CPm gene is 2022 nucleotides long and putatively encodes a protein with estimated size 77.5 kDa. The PYVV CPm gene product is considerably larger than the equivalent proteins encoded by the bipartite criniviruses, Lettuce infectious yellows virus (LIYV) and Cucurbit yellow stunting disorder virus (CYSDV) (52 and 53 kDa, respectively). The PYVV CPm possesses a centralized domain which is absent from both the LIYV and CYSDV CPm counterparts. Pairwise comparisons as well as phylogenetic analysis based on the available amino acid sequences of the CPm of various WTCs, showed that PYVV is closely related to LIYV, CYSDV and also Beet pseudo-yellows virus.
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References
Karasev A.V., Ann Rev Phytopath 38, 293–324, 2000.
Martelli G.P., Agranovsky A.A., Bar-Joseph M., Boscia D., Candresse T., Coutts R.H.A., Dolja V.V., Duffus J.E., Falk B.W., Gonsalves D., Jelkmann W., Karasev A.V., Minafra A., Murant A., Namba S., Niblett C.L., Vetten H.J., Yoshikawa N., in van Regenmortel M.H.V., Fauquet C.M., Bishop D.H.L., Carstens E.B., Estes M.K., Lemon S.M., Maniloff J., Mayo M.A., McGeoch D.J., Pringle C.R., and Wickner R.B., Virus Taxonomy. 7th Report of the International Committee on Taxonomy of Viruses. pp. 949–952. Academic Press, San Diego, CA, USA, 2000
Klaassen V.A., Boeshore M.L., Koonin E.V., Tian T., and Falk B.W., Virology 208, 99–110, 1995.
Livieratos I.C., and Coutts R.H.A., Virus Genes 24, 225–230, 2002.
Yeh H.-H., Tian T., Rubio L., Crawford B., and Falk B.W., J Virol 74, 5762–5768, 2000.
Salazar L.F., Muller G., Querci M., Zapata J.L., and Owens R.A., Ann Appl Biol 137, 7–19, 2000.
Mayo M.A., Arch Virol 5, 1071–1076, 2002.
Woudt L.P., de Rover A.P., de Haan P.T., and van Grinsven M.Q.J.M., Proceedings 6th International Congress of Virology, Glasgow, Scotland, 1993.
Valverde R.A., Nameth S.T., and Jordan R.L., Plant Dis 74, 255–258, 1990.
Livieratos I.C., Katis N., and Coutts R.H.A., Plant Path 47, 362–369, 1998.
Coffin R.S. and Coutts R.H.A., Intervirology 33, 197–203, 1992.
Livieratos I.C., Avgelis A.D., and Coutts R.H.A., Phytopathology 89, 1050–1055, 1999.
Bringloe D.H., Gultyaev A.P., Pelpel M., Pleij C.W.A., and Coutts R.H.A., J Gen Virol 79, 1539–1546, 1998.
Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Miller W., and Lipman D.J., Nucleic Acids Res 25, 3389–3402, 1997.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G., Nucleic Acids Res 25, 4876–4882, 1997.
Page R.D.M., Comp Appl Bios 12, 357–358, 1996.
Wisler G.C., Li R.H., Liu H.-Y., Lowry D.S., and Duffus J.E., Phytopathol 88, 402–409, 1998.
Alzhanova D.V., Napuli A.J., Creamer R., and Dolja V.V., EMBO J. 20, 6997–7007, 2001.
German S., Candresse T., Le Gall O., Lanneau M., and Dunez J., J Gen Virol 73, 767–773, 1992.
Zhu H.-Y., Ling K.S., Goszcynski D.E., McFerson J.R., and Gonsalves D., J Gen Virol 79, 1289–1298, 1998.
Jelkmann W., Fechtner B., and Agranovsky A.A., J Gen Virol 78, 2067–2071, 1997.
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Livieratos, I.C., Muller, G., Salazar, L.F. et al. Identification and Sequence Analysis of Potato yellow vein virusCapsid Protein Minor Gene. Virus Genes 25, 317–322 (2002). https://doi.org/10.1023/A:1020988327197
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DOI: https://doi.org/10.1023/A:1020988327197