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Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes

Nature volume 377, pages 438–441 (1995)Cite this article

Abstract

INXenopus oocytes, as well as other cells, inositol-l,4,5-tris-phosphate (Ins(l,4,5)P3)-induced Ca2+ release1-4 is an excitable process that generates propagating Ca2+ waves5-7 that annihilate upon collision8-12. The fundamental property responsible for excitability13 appears to be the Ca2+ dependency of the Ins(l,4,5)P3 receptor9. Here we report that Ins(l,4,5)P3-induced Ca2+ wave activity is strengthened by oxidizable substrates that energize mitochondria, increasing Ca2+ wave amplitude, velocity and interwave period. The effects of pyruvate/malate are blocked by ruthenium red at the Ca2+ uniporter, by rotenone at complex I, and by antimycin A at complex III, and are subsequently rescued at complex IV by ascorbate tetramethylphenylenediamine (TMPD)14. Our data reveal that potential-driven mitochondrial Ca2+ uptake is a major factor in the regulation of Ins(l,4,5)P3-induced Ca2+ release and clearly demonstrate a physiological role of mitochondria in intracellular Ca2+ signalling.

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Author notes

  1. Laurence S. Jouaville & François Ichas

    Present address: Groupe d'étude des Systémes Biologiques Intégrés, D(BM)2, Université Bordeaux II, 33076, Bordeaux Cedex, France

  2. Ekhson L. Holmuhamedov

    Present address: NASA Ames Research Center, Mail Stop 236-7, Moffett Field, California, 94035, USA

Authors and Affiliations

  1. Department of Neuroscience, University of Virginia, Charlottesville, Virginia, 22908, USA

    Laurence S. Jouaville, François Ichas, Ekhson L. Holmuhamedov, Patricia Camacho & James D. Lechleiter

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  1. Laurence S. Jouaville
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  5. James D. Lechleiter
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Jouaville, L., Ichas, F., Holmuhamedov, E. et al. Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes. Nature 377, 438–441 (1995). https://doi.org/10.1038/377438a0

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