Immobilization of laccase on functionalized multiwalled carbon nanotube membranes and application for dye decolorization

Abdelmageed M. Othman; Elena González-Domínguez; Ángeles Sanromán; Miguel Correa-Duarte; Diego Moldes

doi:10.1039/C6RA18283F

Immobilization of laccase on functionalized multiwalled carbon nanotube membranes and application for dye decolorization

Abdelmageed M. Othman,

^ab Elena González-Domínguez,^c Ángeles Sanromán,

^a Miguel Correa-Duarte

^c and Diego Moldes

*^a

Author affiliations

* Corresponding authors

^a Bioengineering and Sustainable Processes Research Group, Department of Chemical Engineering, Universidade de Vigo, Edificio Isaac Newton, Lagoas Marcosende s/n, 36310 Vigo, Spain
E-mail: diego@uvigo.es, diegomoldes@gmail.com
Tel: +34 986818723

^b Microbial Chemistry Department, National Research Centre, Dokki, 12622 Giza, Egypt

^c Department of Physical Chemistry, Biomedical Research Center (CINBIO), Institute of Biomedical Research of Ourense-Pontevedra-Vigo (IBI), Universidade de Vigo, 36310 Vigo, Spain

Abstract

Myceliophthora thermophila laccase was covalently immobilized on functionalized multiwalled carbon nanotubes (MWNT) arranged over a supporting membrane to obtain a permeable bio-barrier that could be applied in multibatch or continuous processes. Initially, several supporting materials, namely cellulose nitrate, agarose and polyvinyl alcohol, were evaluated as supports for the MWNT. The maximum enzyme loading of 0.286 U mg⁻¹ of MWNT was obtained for cellulose nitrate support supplemented with 0.21 mg MWNT. Immobilized laccase showed good operational stability (above 95% of its initial activity was maintained after 10 cycles of reaction) and exhibited improved stability towards temperature, pH and acetone: for instance, 21% and 49% for free and immobilized laccase, respectively, when incubated in the presence of 20% of acetone (v/v) for 6 h. The immobilized laccase was applied for Reactive Black 5 (RB5) decolorization. Maximum values of RB5 decolorization were observed in the presence of 1-hydroxybenzotriazole as mediator and at pH 5.0, with 68.09 and 84.26% decolorization after 6 and 24 h, respectively.

Article information

https://doi.org/10.1039/C6RA18283F

Article type

Paper

Submitted

18 Jul 2016

Accepted

01 Dec 2016

First published

02 Dec 2016

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RSC Adv., 2016,6, 114690-114697

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Immobilization of laccase on functionalized multiwalled carbon nanotube membranes and application for dye decolorization

Abdelmageed M. Othman, E. González-Domínguez, Á. Sanromán, M. Correa-Duarte and D. Moldes, RSC Adv., 2016, 6, 114690 DOI: 10.1039/C6RA18283F

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Immobilization of laccase on functionalized multiwalled carbon nanotube membranes and application for dye decolorization

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