New Results
Shadow imaging for panoptical visualization of living brain tissue
Yulia Dembitskaya, Andrew K. J. Boyce, Agata Idziak, Atefeh Pourkhalili Langeroudi, Guillaume Le Bourdellès, Jordan Girard, Misa Arizono, Mathieu Ducros, Marie Sato-Fitoussi, Amaia Ochoa de Amezaga, Kristell Oizel, Stephane Bancelin, Luc Mercier, Thomas Pfeiffer, Roger J. Thompson, Sun Kwang Kim, Andreas Bikfalvi, View ORCID ProfileU. Valentin Nägerl
doi: https://doi.org/10.1101/2022.11.03.511028
Yulia Dembitskaya
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Andrew K. J. Boyce
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
2Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada
3Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada
Agata Idziak
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Atefeh Pourkhalili Langeroudi
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Guillaume Le Bourdellès
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Jordan Girard
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Misa Arizono
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
4Department of Pharmacology Kyoto University Graduate School of Medicine
Mathieu Ducros
5Université de Bordeaux, CNRS, INSERM, Bordeaux Imaging Center (BIC), UAR 3420, US 4, F-33000 Bordeaux, France
Marie Sato-Fitoussi
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Amaia Ochoa de Amezaga
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Kristell Oizel
6Université de Bordeaux, INSERM, Bordeaux Institute of Oncology (BRIC), U1312, Bat B2, Allée Geoffroy St Hilaire, 33615 Pessac, France
Stephane Bancelin
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Luc Mercier
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Thomas Pfeiffer
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Roger J. Thompson
2Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada
3Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada
Sun Kwang Kim
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
7Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea
Andreas Bikfalvi
6Université de Bordeaux, INSERM, Bordeaux Institute of Oncology (BRIC), U1312, Bat B2, Allée Geoffroy St Hilaire, 33615 Pessac, France
U. Valentin Nägerl
1Interdisciplinary Institute for Neuroscience, CNRS UMR 5297 and University of Bordeaux, F-33000 Bordeaux, France
Abstract
Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation, do not have cellular resolution or only give a fragmented view. Here, we show how regular light microscopy together with fluorescence labeling of the interstitial fluid in the extracellular space provide comprehensive optical access in real-time to the anatomical complexity and dynamics of living brain tissue.
Competing Interest Statement
The authors have declared no competing interest.
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Posted November 03, 2022.
Shadow imaging for panoptical visualization of living brain tissue
Yulia Dembitskaya, Andrew K. J. Boyce, Agata Idziak, Atefeh Pourkhalili Langeroudi, Guillaume Le Bourdellès, Jordan Girard, Misa Arizono, Mathieu Ducros, Marie Sato-Fitoussi, Amaia Ochoa de Amezaga, Kristell Oizel, Stephane Bancelin, Luc Mercier, Thomas Pfeiffer, Roger J. Thompson, Sun Kwang Kim, Andreas Bikfalvi, U. Valentin Nägerl
bioRxiv 2022.11.03.511028; doi: https://doi.org/10.1101/2022.11.03.511028
Shadow imaging for panoptical visualization of living brain tissue
Yulia Dembitskaya, Andrew K. J. Boyce, Agata Idziak, Atefeh Pourkhalili Langeroudi, Guillaume Le Bourdellès, Jordan Girard, Misa Arizono, Mathieu Ducros, Marie Sato-Fitoussi, Amaia Ochoa de Amezaga, Kristell Oizel, Stephane Bancelin, Luc Mercier, Thomas Pfeiffer, Roger J. Thompson, Sun Kwang Kim, Andreas Bikfalvi, U. Valentin Nägerl
bioRxiv 2022.11.03.511028; doi: https://doi.org/10.1101/2022.11.03.511028
Subject Area
Subject Areas
- Biochemistry (11760)
- Bioengineering (8760)
- Bioinformatics (29216)
- Biophysics (14988)
- Cancer Biology (12105)
- Cell Biology (17417)
- Clinical Trials (138)
- Developmental Biology (9430)
- Ecology (14190)
- Epidemiology (2067)
- Evolutionary Biology (18317)
- Genetics (12246)
- Genomics (16807)
- Immunology (11876)
- Microbiology (28108)
- Molecular Biology (11607)
- Neuroscience (61020)
- Paleontology (452)
- Pathology (1872)
- Pharmacology and Toxicology (3238)
- Physiology (4966)
- Plant Biology (10429)
- Synthetic Biology (2888)
- Systems Biology (7341)
- Zoology (1651)