Single molecule CdSe/ZnS quantum dot and gold nanoparticle detection in live neurons

D. Lasne; L. Cognet; S. Berciaud; G. A. Blab; L. Groc; M. Heine; D. Choquet; B. Lounis

doi:10.1117/12.647788

27 March 2006 Single molecule CdSe/ZnS quantum dot and gold nanoparticle detection in live neurons

D. Lasne, L. Cognet, S. Berciaud, G. A. Blab, L. Groc, M. Heine, D. Choquet, B. Lounis

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 609616 (2006) https://doi.org/10.1117/12.647788
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We applied single-molecule fluorescence microscopy (using organic dyes or semiconductor quantum dots) to study the lateral diffusion of glutamate receptors (AMPA and NMDA) in live synapses. We directly imaged glutamate receptors movements inside and outside synapses of live cultured hippocampal neurons. We could record exchanges of receptors through lateral diffusion between these different membrane compartments. In addition, our data suggest that this lateral diffusion might be regulated by neuronal activity. To overcome the photobleaching problem inherent to fluorescence techniques we recently developed new optical methods for the detection of individual metallic nanoparticles. We can now detect signatures of diffusing AMPA receptors labeled with 10 nm gold nanoparticles on live neurons.

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D. Lasne, L. Cognet, S. Berciaud, G. A. Blab, L. Groc, M. Heine, D. Choquet, and B. Lounis "Single molecule CdSe/ZnS quantum dot and gold nanoparticle detection in live neurons", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 609616 (27 March 2006); https://doi.org/10.1117/12.647788

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KEYWORDS

Receptors

Diffusion

Nanoparticles

Neurons

Gold

Proteins

Molecules

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