A Low-Cost Method for Brain Slice Cultures

Ryuta Koyama; Rieko Muramatsu; Takuya Sasaki; Rie Kimura; Chihiro Ueyama; Makoto Tamura; Naohiro Tamura; Junya Ichikawa; Naoya Takahashi; Atsushi Usami; Maki K. Yamada; Norio Matsuki; Yuji Ikegaya

doi:10.1254/jphs.SC0070119

Short Communications

A Low-Cost Method for Brain Slice Cultures

Ryuta Koyama, Rieko Muramatsu, Takuya Sasaki, Rie Kimura, Chihiro Ueyama, Makoto Tamura, Naohiro Tamura, Junya Ichikawa, Naoya Takahashi, Atsushi Usami, Maki K. Yamada, Norio Matsuki, Yuji Ikegaya

Author information

Ryuta Koyama
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Rieko Muramatsu
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Takuya Sasaki
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Rie Kimura
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Chihiro Ueyama
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Makoto Tamura
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Naohiro Tamura
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Junya Ichikawa
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Naoya Takahashi
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Atsushi Usami
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Maki K. Yamada
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Norio Matsuki
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan
Yuji Ikegaya
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan

Keywords: membrane-interface tissue culture, organotypic culture, hippocampal slice

JOURNAL FREE ACCESS

2007 Volume 104 Issue 2 Pages 191-194

DOI https://doi.org/10.1254/jphs.SC0070119

View "Advance Publication" version (June 8, 2007).

Details

Abstract

Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM^® method.

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