The authors would like to report a typographical error that states the concentration of staurosporine used for the induction of apoptosis is 10 μM. The actual concentration used is 1 μM. The authors confirm that the changes do not affect the results of the experiments.
The typographical error can be found in the following two instances and are corrected here:
In the Figure 8 legend, Panel F (page 17 in the PDF):
“Primary cultures were pre-treated with KA (1 mM) for 2 h prior to adding apoptotic NE-4C cells (treated with 5 μM CM-DiI for 25 min and 1 μM staurosporine for 4 h).”
Under Materials and Methods, in the second paragraph under the ‘Primary Microglia Cultures’ heading (Page 32 in the PDF):
“NE-4C were previously labeled with the membrane marker CM-DiI (5 μM; 10 min at 37°C, 15 min at 4°C; Invitrogen) and treated with staurosporine (1 μM, 4h; Sigma) to induce apoptosis.”
Reference
- 1. Abiega O, Beccari S, Diaz-Aparicio I, Nadjar A, Layé S, Leyrolle Q, et al. (2016) Neuronal Hyperactivity Disturbs ATP Microgradients, Impairs Microglial Motility, and Reduces Phagocytic Receptor Expression Triggering Apoptosis/Microglial Phagocytosis Uncoupling. PLoS Biol 14(5): e1002466. pmid:27228556
Citation: Abiega O, Beccari S, Diaz-Aparicio I, Nadjar A, Layé S, Leyrolle Q, et al. (2016) Correction: Neuronal Hyperactivity Disturbs ATP Microgradients, Impairs Microglial Motility, and Reduces Phagocytic Receptor Expression Triggering Apoptosis/Microglial Phagocytosis Uncoupling. PLoS Biol 14(9): e1002554. https://doi.org/10.1371/journal.pbio.1002554
Published: September 20, 2016
Copyright: © 2016 Abiega et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.