Introduction

Knowledge of daily and routine individual human mobility patterns within urban settings are important for urban planning [1]–[3], developing transportation models [3], promoting healthy lifestyles [4], and understanding infectious disease dynamics [5]–[13]. Measuring mobility at fine spatial and temporal scales through classic data collection methods (e.g., interviews, diaries, direct observations) presents significant challenges, such as marked heterogeneities in the ability of individuals to recall the locations they visit, changes in people's lives that affect their daily mobility (e.g., new partners, change of jobs, school vacation) as well as privacy issues [11], [14]. These challenges can be exacerbated in resource-poor settings [6], [7], [10], [15], [16], such as our study site in Iquitos, Peru, due to the lack of complete and updated address maps (affecting geo-coding of self-reported addresses) and limitations in spatial literacy of interviewed individuals [11]. There is an urgent need to develop and validate easily deployable and culturally-sensitive tools that characterize a person's routine mobility in order to link such information to health outcomes [6], [10], [13], [17], [18]. This is of particular relevance for understanding infectious disease dynamics, given the dominant role mobility has in driving infectious contacts and thus pathogen transmission, emergence, persistence and propagation [5], [6], [8]–[13], [18]–[22].

The wide availability of emerging location-aware technologies such as Global Positioning System (GPS)-phones or data-loggers provides new opportunities to quantify human mobility at fine spatial and temporal scales. Their use in research projects is feasible: they have decreased in cost and size, the technology has improved (i.e., GPS chipsets are more efficient in acquiring and fixing a signal as well as in power consumption) and the units are widely accepted by study populations [6], [10], . Over the past ten years, GPS tracking (often coupled with other sensors) has taken a prominent role in physical activity and exposure research [17], [26], [27]. Their implementation, however, in infectious disease research has been limited in part due to the challenges in linking the positional data generated by such sensors with temporally and spatially discrete locations (i.e., a person's home) where pathogen exposure occurred, and more importantly, the complexities associated with the analysis of the vast amount of data that these sensors can generate. A recent systematic review [17] shows that most studies using GPS to track physical activity involve few participants (<20), track individuals over short time periods (<12 days) and are focused on specific age groups (children vs. adults) or environmental correlates of activity (e.g., park vs. school movement) [17], [27]. GPS-based tracking presents enormous opportunities for improving our understanding of individual space-time activities and how they influence health outcomes, which has been done in various studies [6], [7], [10], [11], [15], [16], [28].

GPS technology, however, also has limitations that need to be addressed before considering it a “gold standard” for mobility research [26]. Rates of GPS data loss can reach 92% due to signal drop-outs, dead batteries, participants not wearing the units, signal loss during the initialization period or misuse of the device [17]. In Stothard et al.'s study in Uganda, the authors found that the track logs of the small, wearable GPS units (i-gotU) were accurate compared to a more sophisticated and costly unit (Garmin Oregon 550t) – discordance of <7 m for the 15 households tested – but there was GPS malfunction in units that was believed to be related to “insufficiently robust hardware for field conditions” possibly due to humidity or quality of the software [10].

As part of a larger study investigating risk for dengue (a human disease caused by a mosquito transmitted virus) in Iquitos, Peru, we simultaneously implemented two methods to capture fine-scale human mobility patterns: GPS data-loggers and semi-structured interviews (SSI). Dengue is a mosquito-transmitted viral disease of humans in tropical and subtropical regions of the world that is a rapidly growing public health problem [29], [30]. The main goal of this observational study was to compare and contrast the information obtained through these two methods to assess the issues affecting data quality, and identify strengths and weaknesses of each approach. We used two methods to analyze GPS data, and compared GPS results obtained via both methods with the results from the SSI.

Methods

Recruitment, Participants, and Study Design

Participant recruitment was not random: we used purposive sampling and focused on two Iquitos neighborhoods participating in an ongoing longitudinal study on dengue epidemiology [32], [36], seeking a balanced number of males and females representing age ranges between 7 and 74 (see Table 1). We only excluded those who planned to spend more than a day outside of Iquitos during the following 14 days. Recruitment was performed by trained local technicians who provided a description of the study together with a pamphlet with specific information about the GPS units and the study in general [16]. In the first phase, conducted between September 2008 and March 2009, 59 participants were asked to use the GPS units at all times for a period of 14 days and respond to the SSI on day 15 asking for all the places they visited while GPS-tracked during those 14 previous days. The research team scheduled an exchange of the GPS units every three days to download data, verify function, and recharge batteries. At the time of GPS unit exchange, participants were asked about their experiences with the GPS, whether they had used it, if it had been forgotten and, if so, on what days. GPS units were programmed to track a person's position (latitude, longitude and time stamp) every 150 seconds. The second phase was conducted in July and August of 2010 with 101 participants, who were asked to follow the same procedures as before; use the GPS unit for 14 days and respond to the SSI on day 15. One component was added in this phase: within 3 days of data collection, survey data was entered into a database and GPS-collected data was processed so that information on the locations identified as visited by each method were overlaid in a Geographic Information System (ArcGIS 10, ESRI). With a series of maps noting the position of each place visited by either method, field technicians returned to the participants within 4–5 days to ask them about any discordant information (i.e., locations on the survey, but not registered on the GPS or vice versa). For Phase 2, the GPS collection frequency was increased to every 15 seconds (45 participants) and 90 seconds (56 participants) to assess the impact of data collection frequencies on GPS-SSI concordance. Whereas with 150 second programming, we could collect and recharge GPS units every 3 days, individuals wearing GPS units programmed at 15 and 90 seconds were provided with a charger and asked to charge the units daily because of the reduction in battery life. Our sample size was sufficient for a descriptive analysis and was limited due to intense participant follow-up for ∼20 days; i.e., recruiting and consenting, distributing GPS units, exchanging charged GPS units and collecting ones losing power, interviewing participants with SSI at day 14, geocoding locations immediately, inputting all data from GPS and SSI to overlay in a GIS, returning to participants for follow up interview. Considering these complexities, participant recruitment was limited to what was logistically feasible for our field teams.

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Table 1. Demographic description of 160 participants for which concurrent semi-structured interviews and GPS tracking were performed.

https://doi.org/10.1371/journal.pntd.0002888.t001

Results

More than half of the 160 enrolled participants were females (58.5%) (Table 1). The lower number of males was due to the difficulty in finding them at home during regular interviewing hours. Recruitment was stratified by age; the age range sampled was 7 to 74 years. Recruitment varied across age groups (range of 25–46 per age group), with 7–18 year olds accounting for 28% of the tracked individuals (Table 1). Although not perfectly balanced among sexes and age groups, the recruited population represents a large and diverse demographic sample of the local population.

Of the 2,566 locations identified by SSI and/or i-Cluster algorithm, 14.3% were concurrently identified by both (i.e., concordant). SSI identified 2.3 times more locations than the i-Cluster algorithm, with residential (42.5%), commercial (26.4%) and educational (10.8%) spaces accounting for the highest degree of concordance between methods (Table 2). A total of 2,047 places were reported in the SSI as visited by all participants over the 14-day tracking period (of these 2047 places mentioned, 1057 were unique places, see Table 2). Most (96.7%) places were located within the urban and peri-urban areas of Iquitos (Figure 1A). Participants reported visiting a median (Q1–Q3) of 12 (9–16) places over the 14-day period, with the number of places not differing significantly between sexes (Wilcoxon rank sum test with continuity correction, W = 3140.5, P = 0.89). The most commonly reported location types on SSI that were not visualized using the i-Cluster algorithm (considering 1609 locations with land-use information) were commercial locations (34.2%) followed by residential (22.1%) and recreational (17.0%) locations (Table 2). The i-Cluster algorithm identified a total of 886 places as visited by participants while tracked (716 unique locations); 98.7% of which were found within the urban and peri-urban areas of Iquitos (Figure 1B). A significantly lower median (Q1–Q3) number of places per participant was registered by the i-Cluster algorithm in comparison to the SSI (7, 4.0–10.0; W = 11990, P<0.001). Residential spaces represented 58.6% of the 454 i-Cluster-identified locations with land-use information that were not reported on the SSI, followed by commercial (11.4%), educational (4.2%), and recreational (3.5%) locations (Table 2). Locations with highest percentage of concordance (i.e., per type of location, the number of concordant sites divided by the total number of sites obtained for that type of location through SSI and/or GPS) were educational settings (24%), followed by residential (19%), other (18%), and religious or market spaces (both at 13%).

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Figure 1. Locations inferred by (A) semi-structured interviews (SSI) and (B) GPS units.

(A) Spatial distribution of all locations reported as visited by 160 participants during a 14-day period. (B) Raw GPS tracks (yellow points) and locations inferred after the application of a data-reduction algorithm (black dots) that assigns each track to a specific location code in the Iquitos GIS.

https://doi.org/10.1371/journal.pntd.0002888.g001

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Table 2. Comparison of number of “concordant” locations identified by semi-structured interviews and GPS from both study phases.

https://doi.org/10.1371/journal.pntd.0002888.t002

When the SSI-reported locations were compared to the raw GPS data (Figure 1), differences in the concordance between methods were observed based on the location type, distance threshold selected, GPS collection frequency and number of GPS points considered to define a visit (Figure 2). Both GPS and SSI had perfect concordance in identifying each participant's home (see Figure 2) at either combination of collection frequency, distance or number of points. There was more concordance for residential sites than non-residential sites at 15 and 90 seconds collection frequency; this difference was minimal at 150 seconds (Figure 2). Not depicted due to the small numbers in each category, there was much variation in concordance when examining by type of location. For example, when examining specific categories such as schools, “other” (ports, storage buildings, empty lots) and lodging places (i.e., rustic “hostels” for visitors from outside Iquitos, or couples might go for a few hours) there was a concordance of 80–100% between methods, whereas other residential places (i.e., friends' or relatives' homes), commercial locations (i.e., shops, markets) and religious buildings (i.e., churches) showed a concordance of 50–80%.

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Figure 2. Concordance between SSI locations and raw GPS positions at different distance buffer thresholds, GPS data collection frequencies, and number of GPS points.

Concordance was expressed as the percentage of locations for which a SSI-GPS match was found.

https://doi.org/10.1371/journal.pntd.0002888.g002

As distance from the SSI reported location increased, the concordance between SSI and raw GPS data increased, independently of the type of location (Figure 2). When at least one raw GPS point was considered (solid lines in Figure 2), concordance between methods was highest at up to 20 meters from each location. Beyond that distance, no dramatic increases in concordance were observed. There was less concordance when we restricted our analysis to 5 GPS points (broken lines) or 10 points (finely broken lines), but the pattern was similar to the line created when 1 point was considered a match. Interestingly, increasing frequency of GPS data collection from 150 to 15 seconds was not associated with a proportional increase in concordance between SSI and GPS (Figure 2). Battery power loss observed at 15 second collection frequency may help explain such results: of the 508 GPS exchanges performed, 56 (11%) of GPS units programmed to collect data every 15 seconds had issues due to battery loss at the time of data download in comparison to 2% (9/379) for GPS units programmed to collect data every 150 sec.

At 20 meters from each SSI location, and when 1 GPS point was considered to define a match, overall concordance averaged 72.6% (SD: 20.7%) for 15 seconds, 65.8% (30.8%) for 90 seconds and 70.3% (23.3%) for 150 seconds (Figure 3). When ten points were required to define a match, concordance was reduced to 59.1% (31.6%), 54.3% (31.0%), and 55.7% (30.7%) respectively (Figure 3). Cemeteries, public buildings, recreational areas and health centers were the location types that consistently showed the lowest concordance values (Figure 3). Increasing the data collection frequency from 15 to 150 seconds did not translate into significant variation in the concordance between SSI and GPS across all location types (average [min-max] variation across locations, 2.3% [0.7%–9%]) (Figure 3).

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Figure 3. Concordance between SSI locations and raw GPS positions at 20 meters from a SSI location.

Concordance is expressed as the proportion of locations for which a SSI-GPS match was found. Panels show values for different location types, combinations of GPS data collection frequencies (15, 90 and 150 seconds) and number of GPS points used to define a visit (1, 5 and 10 points).

https://doi.org/10.1371/journal.pntd.0002888.g003

In comparison to using the raw GPS points (Figure 2), the i-Cluster algorithm evidenced much higher discordance rates for all location types (Table 2). However, this method allowed identifying a total of 519 locations not mentioned in the SSI and not able to be inferred when the raw GPS positions were visualized (Table 2).

In Phase 2, with the subset of 101 participants, we further explored the possible causes of discordance between GPS and SSI. Specifically, within 2–3 days of administering the SSI, we used GIS to develop maps identifying “discordant” SSI and i-Cluster locations (Figure 4) (i.e., locations that were only mentioned in the SSI or only visualized using the GPS data). These maps were used when probing participants about possible causes of discordance. In this phase, regarding locations identified on the SSI, but not detected by GPS (total of 656 locations, Table 3), the most common response to questions about the discordance was an affirmation that these locations had been visited (35.8%) – they could not explain the discordance. The second most common response was that units had “seemed to be turned off” (30%). Indeed, GPS units initially deployed could accidentally be turned off, so respondents who noticed the lack of a flashing blue light inferred correctly. Once this problem was reported, we programmed GPS units to not allow them to be turned off manually, reducing this problem half-way through this study. Other explanations for the discordance included those who admitted forgetting to take units to some locations (12.5%; i.e., rushing out and simply forgetting), not wearing the GPS units to locations that were near their house (3.4%) or to locations where they might get stolen (3.5%), and leaving units home to recharge (4.6%). A small percentage (4.7%) affirmed having the GPS unit in some locations, but questioned whether the placement of the GPS unit in their purse might have impeded the signal.

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Figure 4. Sample map to interview participants about possible causes of discordance between GPS-derived vs. semi-structured interview locations.

Given both types of locations were joined to the Iquitos GIS, the lot code was provided to ease identification of locations in the database. Size of points was proportional to reported or calculated time spent at each location. Inset of map shows locations within the city of Iquitos. GPS-derived locations were obtained using a clustering algorithm.

https://doi.org/10.1371/journal.pntd.0002888.g004

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Table 3. Reasons for discordance given by participants between locations from semi-structured interviews (SSI) and GPS data, from Phase 2 (n = 101).

https://doi.org/10.1371/journal.pntd.0002888.t003

Regarding locations identified on the GPS unit but not mentioned in the SSI (204 locations, Table 3), the most common response was that they simply forgot to mention it in the SSI (38.2%), and a few made the additional observation that they had forgotten this location because it was not part of the regular routine (15.2%). Some locations were not mentioned (until probed directly about them) because they were either transient or en route to another location (22.1%; i.e., a path always taken, a bus stop) or because they were outdoors (13.2%; i.e., outdoor food kiosk). After further examination of the reasons for discordance between SSI and GPS, we identified 75 locations as being affected by technical failures in generating the maps (the locations were not properly mapped or marked the location next door, 57.3% and 42.7% respectively, and hence were incorrectly considered discordant at the time of interview).

Discussion

GPS technology is increasingly used in behavioral research. Its use has moved beyond feasibility tests [15], [35], [37], [38] to the actual use of GPS-enabled devices (often coupled with other sensors such as accelerometers, air pollution sensors or cameras) in studies quantifying various aspects of human mobility and spatial behavior [7], [10], [11]. As the technology continues to be embraced by researchers across disciplines, it is easy to assume that due to the wealth and resolution of the data it provides, some might consider GPS data to be a “gold standard” for mobility research and a replacement of classic survey instruments [35]. By performing a field validation study tracking 160 individuals, we assessed both the limitations and possibilities of GPS technology for mobility research, and provided evidence of multiple sources of error/uncertainty that can affect quality of data in comparison to survey methods. It is important to mention here that based on our experience, we would expect different results with different GPS units, different SSI and other methods of data analysis.

Under perfect conditions of satellite geometry and signal strength, GPS provides very accurate information about the position (latitude, longitude, elevation, time of day) of any stationary object on earth. Wearable GPS devices provide all the essential pieces of information to reconstruct and quantify human movement: positions associated to places visited, time stamp for each potential visit, and routes followed to connect visits. Given technical (e.g., signal noise, multipath errors, signal obstruction inside buildings, battery life) and human behavioral limitations (e.g., compliance of use, individuals forgetting to take or charge units), GPS signals are prone to error and estimates of mobility parameters that they generate are considered uncertain. Signal processing algorithms have been developed to reduce such errors and improve interpretation of complex data [39]–[44]. In our study, the application of a signal clustering algorithm (i-Cluster) allowed identifying locations where individuals spent their time, but also added significant uncertainty by flagging locations transiently visited (e.g., a bus stop; 35.3%). Such errors were the main contributor to the 85.7% discordance between methods observed when i-Cluster inferred locations were considered. Because most research describing automated algorithms rely on single (or few) days of data or low sample sizes [39]–[44], the errors found by our study are a likely outcome of the type of error those algorithms may encounter if applied within the same context. Our results can be used as a guide for the development of improved and more accurate methods for GPS location extraction and human movement quantification.

An interesting finding was that higher GPS collection frequencies (e.g., 15 seconds) were not associated with a proportional and significant increase in concordance between methods. Issues of battery life, not securing the “off” option at the start of the study (remedied quickly), and compliance of participants in charging the units compromised the quality of data collected. Similar issues were observed across multiple studies quantifying physical activity [17], [35]. Implementing GPS-enabled smart-phones could have reduced the issue of battery loss, because there is more motivation for individuals to charge the phones overnight and to use them during the day. Because Iquitos is slowly making its transition into smart-phone technologies, different issues were pointed out by a subset of 10 participants when asked about the possibility of using GPS-phones instead of data-loggers: (a) older individuals were intimidated by the technology and by the possibility of having the units stolen (the latter was a concern shared by individuals across all age groups), and (b) school age children mentioned they are not allowed to take phones to elementary or high school or locations where their phones could be taken by older children [7]. When cell-phones can be properly deployed they can provide valuable information. For instance, in Canada a study comparing GPS data collected by cell-phones and self-reported surveys reported (using rudimentary indices of concordance such as convex hulls and kernel density estimations) that 75% of questionnaire-reported activity locations were located within 400 meters of an activity location recorded on the GPS track [26]. In weighting the possibility of adopting novel technologies, consideration of cultural and local concerns will be key for both GPS and SSI instruments [13], [16], [18].

Turning the large amounts of raw GPS positional data into meaningful locations individuals visited is another challenge. Unprocessed raw GPS data can be used to either describe zones or areas in which individuals spend their time or to assess the accuracy of the GPS in identifying precise locations against information provided by another method (i.e., locations identified by SSI). In our study we implemented a simple algorithm based on an agglomerative clustering method (i-Cluster) to identify locations visited by individuals carrying a GPS unit. Our analysis shows that the algorithm presents low levels of sensitivity and specificity in identifying places reported as visited by participants. This poor performance could be due to: (a) the algorithm's limited ability to account for changes in accuracy of the GPS signal or to the occurrence of intermittent positions as a consequence of GPS signal loss and (b) the fact that not all reported locations were actually visited by participants while tracked. More complex methods of location extraction that account for signal errors, such as hierarchical dynamic Bayesian network models [39], [41], [44], are being currently developed and are viewed as a promising means of reducing the uncertainty associated with the identification of locations visited by participants [39], [44]. Once those methods are validated, their integration into health research applications will increase our ability to accurately infer the location of potential infectious disease exposure areas.

Classic methods (surveys, diaries) have long been considered too limited to quantify behavior due to marked heterogeneities in the ability of individuals to recall the locations they visit, interviewer error, behavior changes and issues associated to privacy [35]. By working with the local community, addressing potential cultural barriers and concerns and adapting the language of interviews, we developed a culturally-sensitive SSI to quantify movement (and potential exposure to dengue). Our comparative analysis shows that, for a 14-day recall period, interviews provide accurate estimates of the locations visited by people (of a total of 892 locations for which we investigated causes of discordance, only 109 [12.2%] were visited and not reported). The SSI not only identified places, but also characterized the context of visits (i.e., grandmother's house), information impossible to obtain directly from GPS. SSI data entry and processing are much more straightforward and faster than of GPS: (a) maps with marked locations were digitized in the Iquitos GIS and each premise reported as visited was assigned a location code and (b) the location code was then linked to the database containing all the SSI information. We concluded that a validated survey instrument that can be adapted to different contexts can be used to understand the role of human mobility in infectious disease dynamics.

We encountered several limitations in our study design. Although our sample size was relatively large, the low numbers of participants assigned to each age group precluded statistical tests to look at different causes of discordance. Given that we needed to obtain results quickly to ask participants about possible causes of discordance, we relied on a single GPS data reduction algorithm (i-Cluster). As observed on the survey (Table 3), most of the discordant records occurred due to this algorithm providing false positive or negative results. Since the time this study was performed, new and more sophisticated methods to process GPS data have been developed [39]–[44], as well as more accurate and less error-prone GPS units have likely become available. Future research will involve performing comparative studies to quantify sensitivity/specificity as well as applicability to specific study questions. Also, we considered that our concordance estimates could be, in part, dependent on size and placement of houses in Iquitos. An average household in this city measures 5 m in width, which is within the mean error of a GPS (5–10 m). This could explain the high percent (∼60%) of residences identified by GPS that were not reported on the SSI. Thus, accuracy in identifying locations is not only dependent on the factors explained above, but also on key attributes of the urban landscape (e.g., household size, prevailing building material, density of high-rise buildings, vegetation cover). We did not test for differences in the SSI results of participants with more contact with our research team (i.e., those with more frequent GPS exchanges due to differing data collection times) compared to those with minimal contact. We do not expect differences, however, because contact was focused on the GPS exchange and SSI questions about their movement and activities were only asked at the end of the 14 day period. None of the participants, therefore, had an advantage over others regarding the types of questions they would be asked. We also did not estimate nor compare the cost and technical expertise to apply and process by these methods. Both the GPS and SSI capture very complex data. GPS data is in digital form, but needs to be processed. SSI data needs to be verified (i.e., in our study, someone might go to a location described to geocode the location), entered and mapped. There were costs associated to purchasing GPS units (∼$49/unit), training personnel to set and distribute units, downloading and analyzing the GPS data. Similarly, there were costs associated to developing, refining and improving the SSI, training personnel to apply it, and entering the data in a GIS system. Ultimately, decisions regarding using an SSI or GPS units in a study depend strongly on the study question and the urban context, because both SSI and GPS can provide different but equally valuable information that need to be carefully weighted at the planning stage.

For infectious diseases in general, and vector-borne diseases in particular, the need to tie potential exposure to specific locales requires the retrospective investigation of multiple routes of pathogen transmission. Survey instruments like the one we developed in this study not only provide accurate information of places visited, but can also be used to retrospectively infer the likely location where infection occurred [5]. This need to tie exposure to a specific place(s) has limited the use of GPS technology in infectious disease research, but GPS technology could be used in prospective movement studies or in studies obtaining information provided by phone companies. As observed in our study, once locations are identified, the raw GPS positions can be analyzed to quantify temporal patterns of mobility (days and times a person visits such locations, regularity of visits, overlap with other tracked individuals) and to accurately quantify routines and movement of a large segment of a population. This way, key information about mobility and behavior can be inferred and used to parameterize mathematical models that allow better forecasting of disease transmission or design policies targeting activities or segments of the population at greatest risk.

No gold standard exists for obtaining and analyzing human mobility data, instead different errors may occur with different methods. Despite the continually improving accuracy available with GPS, barriers persist, including: behavioral aspects (i.e., people remembering to use the unit), technical aspects (i.e., accuracy of 5–10 meters in a location with houses averaging 5 meters width), and analytical aspects (i.e., differences in concordance based on method of analyzing complex data as reported in this article). The SSI is not a gold standard either. Even with the possible drawback of more locations reported than true (i.e., false positives), compared to GPS units, the SSI provided more true locations, more context about locations, and data were easier to process and analyze. For our study, in which we needed to identify locations retrospectively for possible exposure to dengue virus, the SSI was the only choice because of the logistical and financial difficulty of fitting GPS units on a large sample and, even if that had been possible, being able to quickly identify locations recently visited within a short enough time frame to initiate our possible exposure investigations. For now, SSI remains the most comprehensive method to identify such locations.

Supporting Information

Acknowledgments

This study required a lot of detailed work and follow up, helping study participants with triggers that would remind them of specific locations visited, and the arduous task of finding locations and geocoding each. It would have been impossible to do without the careful work of the NAMRU-6 movement and entomology teams. The movement team consisted of Wendy Lorena Quiroz Flores, Esther Jennifer Rios Lopez, Shirly M. Guedez Gonzales, and Wilder Carrasco Huaman. The entomology team consisted of Jimmy Castillo Pizango, Fernando Chota Ruiz, Victor Elespuru Hidalgo, Jose Espinoza Benavides, Rusbel Huiñapi Tamani, Guillermo Iñapi Huaman, Nestor Nonato Lancha, Edson Pilco Mermao, Angel Puertas Lozano, Federico Reategui Viena, Manuel Ruiz Rioja, Juan Luis Sifuentes Rios, and Abner Varzallo Lachi, under the supervision of Gerson Perez Rodriguez. The unit that handled downloading GPS data and ensuring they were functioning well included Rommel Vasquez Alves and Jimmy Roberto Espinoza Benavides. Jhon Elvis Ramirez Paredes assisted with the entry of data into the GIS system, following up with any inconsistencies and problems we encountered. Parisa Nourani, Emory student, assisted with overlapping GPS and SSI data into GIS for quick comparison and follow up by field team. Gabriela Vazquez La Torre also assisted in diverse logistical functions related to this work over the past years. Finally, we would like to thank the residents of Iquitos for their support and participation in this study. This study counted with the approval of the Loreto Regional Health Department.

Copyright Statement

Authors Eric S. Halsey and Tadeusz Kochel are U.S. military service members. This work was prepared as part of their official duties. Title 17 U.S.C. § 105 provides that ‚Copyright protection under this title is not available for any work of the United States Government’. Title 17 U.S.C. § 101 defines a U.S. Government work as a work prepared by a military service members or employees of the U.S. Government as part of those persons’ official duties.

Disclaimer

The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government.