Abstract
Tritrichomonas foetus, a parasite of cattle reproductive system, has been recently discovered as a cause of disease in cats in many countries. T. foetus infects and colonizes cat’s ileum, caecum, colon and can lead to enteritis. This paper presents the first clinical case of cat intestinal trichomonosis caused by T. foetus in Poland. The material for this study was a smear collected from a 6-month-old male British Shorthair cat. The presence of parasitic protozoan was determined via microscopic examination and confirmed by amplification of T. foetus rDNA using polymerase chain reaction (PCR) technique. In the first PCR reaction, a DNA of Trichomonadidae was identified and in the second PCR, T. foetus was detected. The T. foetus positive products from the second PCR reaction were sequenced. Interpretation of the sequencing results of obtained amplicons by comparing them with the GenBank database proved that the causative agent, in this case, was T. foetus.
References
Bisset S.A., Gowan R.A., O’Brien C.R., Stone M.R., Gookin J.L. 2008. Feline diarrhoea associated with Tritrichomonas cf. foetus and Giardia co-infection in an Australian cattery. Australian Veterinary Journal, 86, 440-443. DOI:10.1111/j.1751-0813.2008.00356.x 10.1111/j.1751-0813.2008.00356.xSearch in Google Scholar PubMed
BonDurant R.H., Campero C.M., Anderson M.L., Hoosear K.A.. 2003. Detection of Tritrichomonas foetus by polymerase chain reaction in culture isolates, cervicovaginal mucus, and formalin-fixed tissues from infected heifers and fetuses. Journal Veterinary Diagnostic Investigation, 15, 579-58410.1177/104063870301500613Search in Google Scholar PubMed
Carvalho K.P., Gadelha A.P. 2007. Effects of three benzimidazoles on growth, general morphology and ultrastructure of Tritrichomonas foetus. FEMS Microbiology Letters, 275, 292-300. DOI: 10.1111/j.1574-6968.2007.00897.x 10.1111/j.1574-6968.2007.00897.xSearch in Google Scholar PubMed
Da Silva N.S., Ribeiro Cde M., Machado A.H., Pacheco-Soares C. 2007. Ultrastructural changes in Tritrichomonas foetus after treatments with A1PcS4 and photodynamic therapy. Veterinary Parasitology, 146, 175-181. DOI:10.1016/j.vetpar.2007.02.00610.1016/j.vetpar.2007.02.006Search in Google Scholar PubMed
Felleisen R.S.J., Lambelet N., Bachmann P., Nicolet J., Muller N., Gottstein B. 1998. Detection of Tritrichomonas foetus by PCR and DNA Enyzme Imunnoassay Based on rRNA Gene Unit Sequences. Journal Clinical Microbiology, 36, 513-51910.1128/JCM.36.2.513-519.1998Search in Google Scholar PubMed PubMed Central
Foster D.M., Gookin J.L., Poore M.F., Stebbins M.E., Levy M.G. 2004 Outcome of cats with diarrhoea and Tritrichomonas foetus infection. Journal American Veterinary Medical Association, 225, 888-89210.2460/javma.2004.225.888Search in Google Scholar PubMed
Frey C.F., Schild M., Hemphill A., Stunzi P., Muller N., Gottstein B., Burgener I.A.. 2009. Intestinal Tritrichomonas foetus infection in cats in Switzerland detected by in vitro cultivation and PCR. Parasitology Research, 104, 783-788. DOI: 10.3347/kjp.2010.48.3.24710.3347/kjp.2010.48.3.247Search in Google Scholar PubMed PubMed Central
Gookin J.L., Breitschwerdt E.B., Levy M.G., Gager R.B., Benrud J.G. 1999. Diarrhea associated with trichomonosis in cats. Journal American Veterinary Medical Association, 215, 1450-4Search in Google Scholar
Granger B.L., Warwood S.J., Benchimol M., De Souza W. 2000. Transient invagination of flagella by Tritrichomonas foetus. Parasitology Research, 86, 699-709. DOI: 10.1007/PL 00008555Search in Google Scholar
Gray S.G., Hunter S.A., Stone M.R., Gookin J.L. 2010. Assessement of reproductive tract disease in cats at risk for Tritrichomonas foetus infection. AVMA - American Journal of Veterinary Research, 71, 76-81. DOI: 10.2460/ajvr.71.1.7610.2460/ajvr.71.1.76Search in Google Scholar PubMed
Gunn-Moore D.A., McCann T.M., Reed N., Simpson K.E., Tennant B. 2007. Prevalence of Tritrichomonas foetus infection in cats with diarrhoea in the UK. Journal Feline Medicine Surgery, 9, 214-21810.1016/j.jfms.2007.01.003Search in Google Scholar PubMed
Hammond D.M., Bartlett D.E. 1943. The distribution of Trichomonas foetus in the preputial cavity of infected bulls. American Journal Veterinary Research, 4, 143-149Search in Google Scholar
Holliday M., Deni D., Gunn-Moore D.A.. 2009. Tritrichomonas foetus infection in cats with diarrhoea in a rescue colony in Italy. Journal of Feline Medicine and Surgery, 11, 131-134. DOI: 10.1016/j.jfms.2008.06.00410.1016/j.jfms.2008.06.004Search in Google Scholar PubMed
Honigberg B.M. 1978. Parasitic protozoa. II. Intestinal flagellates, histomonads, trichomonads, amoeba, opalinids and ciliates. Academic Press, Inc New York, N.Y.Search in Google Scholar
Klein B., Langbein-Detsch I., Muller E., Heusinger A. 2010. Pravalenz von Tritrichomonas foetus in Kotproben von Katzen mit Durchfall aus Deutschland. Kleintiermedizin, 48, 247-251Search in Google Scholar
Kuehner K.A., Marks S.L., Kass P.H., Sauter L.C., Grahn R.A., Barutzki D., Hartman K. 2011. Tritrichomonas foetus infection in purebred cats in Germany: prevalence of clinical signs and the role of co-infection with other enteroparaistes. Journal Feline Medicine Surgery, 13, 251-258. DOI: 10.1016/ j.jfms.2010.12.00210.1016/j.jfms.2010.12.002Search in Google Scholar PubMed
Lim S., Park S.I., Ahn K.S., Oh D.S., Ryu J.S., Shin S.S. 2010. First Report of Feline Intestinal Trichomoniasis Caused by Tritrichomonas foetus in Korea. Korean Journal Parasitology, 48, 247-251. DOI: 10.3347/kjp.2010.48.3.24710.3347/kjp.2010.48.3.247Search in Google Scholar PubMed PubMed Central
Profizi C., Cian A., Meloni D., Hugonnard M., Lambert V., Groud K., Gagon A.C., Viscogliosi E., Zenner L. 2013. Prevalence of Tritrichomonas foetus infections in French catteries. Veterinary Parasitology, 196, 50-55. DOI: 10.1016/j.vetpar.2013.01.02110.1016/j.vetpar.2013.01.021Search in Google Scholar PubMed
Stauffer S.H., Birkenheuer A.J., Levy M.G., Marr H., Gookin J.L. 2008. Evaluation of four DNA extraction methods for the detection of Tritrichomonas foetus in feline stool specimens by polymerase chain reaction. Journal of Veterinary Diagnostic Investigation, 20, 639-641. DOI:10.1177/10406387080200051810.1177/104063870802000518Search in Google Scholar PubMed
Stockdale H.D., Dillon A.R., Newton J.C., Bird R.C, Bondurant R.H., Deinnocentes P., Barney S., Bulter J., Land T., Spencer J.A., Linday D.S., Blagburn B.L. 2008. Experimental infection of cats (Felis catus) with Tritrichomonas foetus isolated from cattle. Veterinary Parasitology, 154, 156-161. DOI: 10.1016/j.vetpar.2008.02.02410.1016/j.vetpar.2008.02.024Search in Google Scholar PubMed
Stockdale H.D., Givens M.D., Dykstra C., Blagburn L. 2009. Tritrichomonas foetus infections in surveyed pet cats. Veterinary Parasitology, 160: 13-17. DOI: 10.1016/j.vetpar.2008.10.09110.1016/j.vetpar.2008.10.091Search in Google Scholar PubMed
Tysnes K., Gjerde B., Nodtvedt A., Skancke E. 2011. A cross-sectional study of Tritrichomonas foetus infection among healthy cats at shows in Norway. Acta Veterinaria Scandinavica, 53(39), 1-6. DOI: 10.1186/1751-0147-53-3910.1186/1751-0147-53-39Search in Google Scholar PubMed PubMed Central
Walden H.S, Dykstra Ch., Dillon A., Rodning S., Givens D., Bird R., Lindsay D. 2013. A new species of Tritrichomonas (Sarcomastigophora: Trichomonida) from the domestic cat (Felis catus). Parasitology Research, 112, 2227-2235. DOI: 10.1007/s00436-013-3381-810.1007/s00436-013-3381-8Search in Google Scholar PubMed
Walker R.L., Hayes D.C., Sawyer S.J., Nordhausen R.W., Van Hoosear K.A., BonDurant R.H. 2003. Comparison of the 5,8S rRNA gene and internal transcribed spacer regions of trichomonadid protozoa recovered from the bovine preputial cavity. Journal Veterinary Diagnostic Investigation, 15, 14-2010.1177/104063870301500104Search in Google Scholar PubMed
Xenoulis P.G. 2011. Tritrichomonas foetus: a new cause of diarrhea in cats in Greece. Journal of Hell Veterinary Medicine Society, 62(2), 132-140. DOI: 10.1016/j.jfms.2010.05.01010.1016/j.jfms.2010.05.010Search in Google Scholar PubMed
Yaeger M.J., Gookin J.L. 2005. Histologic Features associated with Tritrichomonas foetus- induced Colitis in Domestic Cats. Veterinary Pathology, 42, 797-804 10.1354/vp.42-6-797Search in Google Scholar PubMed
W. Stefański Institute of Parasitology, PAS