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Licensed Unlicensed Requires Authentication Published by De Gruyter March 18, 2015

Effect of Withania somnifera (Ashwagandha) root extract on amelioration of oxidative stress and autoantibodies production in collagen-induced arthritic rats

  • Mahmood Ahmad Khan , Mythily Subramaneyaan , Vinod Kumar Arora , Basu Dev Banerjee and Rafat Sultana Ahmed EMAIL logo

Abstract

Background: Rheumatoid arthritis is an inflammatory autoimmune disorder. Withania somnifera Dunal (Solanaceae) (WS), is a common medicinal plant used in traditional systems of medicine for the treatment of arthritis, and is an ingredient of anti-arthritic polyherbal formulations such as Habb-e-Asgand® and Arthritin™. In the present study, we evaluated the antioxidant and anti-arthritic activity of aqueous extract of WS root (WSAq) in collagen-induced arthritic (CIA) rats.

Methods: CIA rats were treated by using three doses of WSAq (100, 200, 300 mg/kg b. wt., orally) and methotrexate (MTX, 0.25 mg/kg b. wt. i.p.) as a standard reference drug for 20 days. The anti-arthritic effect was assayed by measuring the arthritic index, autoantibodies such as rheumatoid factor (RF), anti-cyclic citrullinated peptide antibody (a-CCP), anti-nuclear antibody (ANA), anti-collagen type II antibody (a-CII) and inflammatory marker like C-reactive protein (CRP). The oxidative stress parameters were also measured.

Results: Treatment with WSAq resulted in a dose-dependent reduction in arthritic index, autoantibodies and CRP (p<0.05) with maximum effect at dose of 300 mg/kg b. wt. and the results were comparable to that of MTX-treated rats. Similarly, oxidative stress in CIA rats was ameliorated by treatment with different doses of WSAq, as evidenced by a decrease in lipid peroxidation and glutathione-S-transferase activity and an increase in the glutathione content and ferric-reducing ability of plasma (p<0.05).

Conclusions: The results showed that WSAq exhibited antioxidant and anti-arthritic activity and reduced inflammation in CIA rats and suggests the potential use of this plant in the treatment of arthritis.

Funding statement: Funding: The authors are thankful to the Department of Biotechnology (DBT), Ministry of Science & Technology (Govt. of India) for providing financial support (No.BT/PR4612/MED/30/812/2012).

Research funding: The authors are thankful to the Department of Biotechnology (DBT), Ministry of Science & Technology (Govt. of India) for providing financial support (No.BT/PR4612/MED/30/812/2012).

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

1. HitchonCA, El-GabalawyHS. Oxidation in rheumatoid arthritis. Arthritis Res Ther2004;6:26578.10.1186/ar1447Search in Google Scholar

2. OlsenNJ, SteinCM. New drugs for rheumatoid arthritis. N Engl J Med2004;350:216779.10.1056/NEJMra032906Search in Google Scholar

3. NaiduPS, SinghA, KulkarniSK. Effect of Withania somnifera root extract on haloperidol-induced orofacial dyskinesia: possible mechanisms of action. J Med Food2003;6:10714.10.1089/109662003322233503Search in Google Scholar

4. RasoolM, VaralakshmiP. Immunomodulatory role of Withania somnifera root powder on experimental induced inflammation: an in vivo and in vitro study. Vascul Pharmacol2006;44:40610.10.1016/j.vph.2006.01.015Search in Google Scholar

5. MirBA, KhazirJ, MirNA, TuH, BotanicalKS. Chemical and pharmacological review of Withania somnifera (Indian ginseng): an Ayurvedic medicinal plant. Indian J Drugs Dis2012;1:14760.Search in Google Scholar

6. SubramaneyaanM, YasmeenS, AroraVK, TripathiAK, BanerjeeBD, AhmedRS. Safety evaluation and therapeutic efficacy of habb-e-asgand, a commonly used antirheumatic polyherbal unani formulation. Altern Ther Health Med2013;19:529.Search in Google Scholar

7. CampoGM, AvenosoA, CampoS, FerlazzoAM, AltavillaD, CalatroniA. Efficacy of treatment with glycosaminoglycans on experimental collagen-induced arthritis in rats. Arthritis Res Ther2003;5:12231.Search in Google Scholar

8. HolmdahlR, CarlsenS, MikulowskaA, VestbergM, BrunsbergU, HanssonA, et al. Genetic analysis of murine models for rheumatoid arthritis. In: AdolphoKW, editor. Human genome methods. New York: CRC Press,1998:215.Search in Google Scholar

9. HultqvistM, OlofssonP, GeldermanKA, HolmbergJ, HolmdahlR. A new arthritis therapy with oxidative burst inducers. PLoS Med2006;3:e348.10.1371/journal.pmed.0030348Search in Google Scholar

10. SatohK. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta1978;90:3743.10.1016/0009-8981(78)90081-5Search in Google Scholar

11. TietzeF. Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem1969;27:50222.10.1016/0003-2697(69)90064-5Search in Google Scholar

12. HabigWH, PabstMJ, JakobyWB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem1974;249:71309.Search in Google Scholar

13. BenzieIF, StrainJJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol1999;299:1527.10.1016/S0076-6879(99)99005-5Search in Google Scholar

14. RaoJK, MihaliakK, KroenkeK, BradleyJ, TierneyWM, WeinbergerM. Use of complementary therapies for arthritis among patients of rheumatologists. Ann Intern Med1999;131:40916.10.7326/0003-4819-131-6-199909210-00003Search in Google Scholar PubMed

15. PandeyS. Various techniques for the evaluation of anti arthritic activity in animal models. J Adv Pharm Technol Res2010;1:16471.Search in Google Scholar

16. BendeleA. Animal models of rheumatoid arthritis. J Musculoskelet Neuronal Interact2001;1:37785.Search in Google Scholar

17. JouIM, ShiauAL, ChenSY, WangCR, ShiehDB, TsaiCS, et al. Thrombospondin 1 as an effective gene therapeutic strategy in collagen-induced arthritis. Arthritis Rheum2005;52:3944.10.1002/art.20746Search in Google Scholar PubMed

18. RhodesB, FürnrohrBG, VyseTJ. C-reactive protein in rheumatology: biology and genetics. Nat Rev Rheumatol2011;7:2829.10.1038/nrrheum.2011.37Search in Google Scholar

19. PavalJ, KaitheriSK, PotuBK, GovindanS, KumarRS, NarayananSN, et al. Comparing the anti-arthritic activities of the plants Justicia gendarussa burm F. and Withania somnifera linn. Int J Green Pharm2009;3:2814.10.4103/0973-8258.59732Search in Google Scholar

20. SabinaEP, ChandalS, RasoolMK. Inhibition of monosodium urate crystal-induced inflammation by withaferin A. J Pharm Pharm Sci2008;11:4655.10.18433/J35K58Search in Google Scholar

21. FilippinLI, VercelinoR, MarroniNP, XavierRM. Redox signalling and the inflammatory response in rheumatoid arthritis. Clin Exp Immunol2008;152:41522.10.1111/j.1365-2249.2008.03634.xSearch in Google Scholar

22. KaratasF, OzatesI, CanatanH, HalifeogluI, KaratepeM, ColaktR. Antioxidant status & lipid peroxidation in patients with rheumatoid arthritis. Indian J Med Res2003;118:17881.Search in Google Scholar

23. LeonavičienėL, BradūnaitėR, VaitkienėD, VasiliauskasA, KeturkienėA. Collagen-induced arthritis and pro-/antioxidant status in Wistar and Lewis rats. Biologija2008;54:290300.10.2478/v10054-008-0059-8Search in Google Scholar

24. MacdonaldIO, OlusolaOJ, OsaigbovoUA. Effects of chronic ethanol administration on body weight, reduced glutathione (GSH), malondialdehyde (MDA) levels and glutathione-s-transferase activity (GST) in rats. N Y Sci J2010;3:3947.Search in Google Scholar

25. SubramaneyaanM, JainS, YadavC, AroraVK, BanerjeeBD, AhmedRS. Quinalphos induced oxidative stress and histoarchitectural alterations in adult male albino rats. Environ Toxicol Pharmacol2012;34:6738.10.1016/j.etap.2012.10.003Search in Google Scholar

26. DhuleyJN. Adaptogenic and cardioprotective action of ashwagandha in rats and frogs. J Ethnopharmacol2000;70:5763.10.1016/S0378-8741(99)00177-4Search in Google Scholar

27. PattisonDJ, WinyardPG. Dietary antioxidants in inflammatory arthritis: do they have any role in etiology or therapy?Nat Clin Pract Rheumatol2008;4:5906.10.1038/ncprheum0920Search in Google Scholar PubMed

28. GriffithsHR. Is the generation of neo-antigenic determinants by free radicals central to the development of autoimmune rheumatoid disease?Autoimmun Rev2008;7:5449.10.1016/j.autrev.2008.04.013Search in Google Scholar PubMed

29. BashirS, HarrisG, DenmanMA, BlakeDR, WinyardPG. Oxidative DNA damage and cellular sensitivity to oxidative stress in human autoimmune diseases. Ann Rheum Dis1993;52:65966.10.1136/ard.52.9.659Search in Google Scholar PubMed PubMed Central

30. SongYW, KangEH. Autoantibodies in rheumatoid arthritis: rheumatoid factors and anticitrullinated protein antibodies. QJM2010;103:13946.10.1093/qjmed/hcp165Search in Google Scholar PubMed PubMed Central

31. ChamundeeswariD, VaijayanthiV, UmamaheswariS, GandhimathiC, KarnaSL, MeignanamE, et al. Antioxidant activity of arthritin – a polyherbal formulation. Indian J Exp Biol2006;44:4037.Search in Google Scholar

32. De BandtM, GrossinM, DrissF, PincemailJ, Babin-ChevayeC, PasquierC. Vitamin E uncouples joint destruction and clinical inflammation in a transgenic mouse model of rheumatoid arthritis. Arthritis Rheum2002;46:52232.10.1002/art.10085Search in Google Scholar PubMed

33. KhareCP. Indian medicinal plants – an illustrated dictionary. New Delhi, India: Springer (India) Pvt. Ltd, 2007.10.1007/978-0-387-70638-2Search in Google Scholar

Received: 2014-12-6
Accepted: 2015-2-25
Published Online: 2015-3-18
Published in Print: 2015-6-1

©2015 by De Gruyter

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