Vet Med - Czech, 2015, 60(7):368-375 | DOI: 10.17221/8384-VETMED

Analyses of anaesthesia with ketamine combined with different sedatives in ratsOriginal Paper

A.M. Molina, M.R. Moyano, J.M. Serrano-Rodriguez, N. Ayala, A.J. Lora, J.M. Serrano-Caballero
Veterinary Faculty, University of Cordoba, Cordoba, Spain

The goal of this study was to establish an easy-to-perform, reliable, and safe protocol for intraperitoneal injection anaesthesia in rats, as well as an easy method to assess anaesthesia depth under routine clinical conditions. Seventy Wistar rats (35 males and 35 females) were used to evaluate intraperitoneal anaesthesia with ketamine (75 mg/kg) combined with one of the following central nervous system depressors: acepromazine (2.5 mg/kg), diazepam (5 mg/kg), medetomidine (0.5 mg/kg), midazolam (5 mg/kg) and xylazine (2.5 mg/kg). Significant differences were found between the combinations in ataxia, recovery and righting parameters, as well as respiratory and heart rates and haemoglobin saturation by oxygen, whereas significant differences between sexes for the parameters of ataxia, hypnosis, recovery, righting and exploratory behaviour were observed upon waking. The best results for parameters such as induction, and maintenance and recovery from anaesthesia (more regular and stable) were observed with α2-agonists: ketamine/xylazine in males and ketamine/medetomidine in females.

Keywords: anaesthesia; rats; ketamine; sedatives

Published: July 31, 2015  Show citation

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Molina AM, Moyano MR, Serrano-Rodriguez JM, Ayala N, Lora AJ, Serrano-Caballero JM. Analyses of anaesthesia with ketamine combined with different sedatives in rats. Vet Med-Czech. 2015;60(7):368-375. doi: 10.17221/8384-VETMED.
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