Acute radiation sickness in sheep affected by respiratory diseases

«Radiation and Risk», 2017, vol. 26, No. 4, pp.124-131

DOI: 10.21870/0131-3878-2017-26-4-124-131

Authors

Sarukhanov V.Ya. – Senior Researcher, C. Sc., Biol. Russian Scientific Research Institute of Radiology and Agroecology, Obninsk. Contacts: Kievskoe Sh., 109 km, Obninsk, Kaluga region, Russia, 249032. Tel.: 8 (484) 399-69-38; e-mail:  This email address is being protected from spambots. You need JavaScript enabled to view it. .
Isamov N.N. – Head of Lab., C. Sc., Biol. Russian Scientific Research Institute of Radiology and Agroecology, Obninsk.
Kobyalko V.O. – Head of Lab., C. Sc., Biol. Russian Scientific Research Institute of Radiology and Agroecology, Obninsk.
Bastrakova L.A. – Lead. Engineer. Russian Scientific Research Institute of Radiology and Agroecology, Obninsk.

Abstract

It is common knowledge that reduction of immunologic reactivity can increase susceptibility of an organism to exogenous infections and risk of death of animals following exposure to sublethal radi-ation doses. Respiratory diseases also reduce immunologic reactivity of an organism. Study of radiation sickness in animals affected by respiratory diseases will allow researchers to estimate possible losses of livestock due to radiological emergency. The article describes results of study of acute radiation sickness developed in sheep affected by chronic respiratory diseases. Experimental data were obtained at the Research Institute of Radiology and Agroecology, Obninsk. Healthy (control) sheep (5) and the animals (5) with chronic respiratory diseases used in the study were brought from farms of Kaluga oblast. All species had veterinary certificates, were quarantined at the institutional vivarium for 20 days. The use of the laboratory animals was governed by the Russian national standard GOST 33215-2014 determining laboratory animals care, management, housing conditions and performance of research procedures. The animals were gamma-irradiated, radiation dose was 4 Gy, radiation source – ¹³⁷Cs, doserate 1 Gy/h. Irradiation ripple was less than 10-15%. Blood was taken in every 5 days in fasting state in mornings before and after irradiation during the follow up period of 30 days. health status of the animals was estimated by clinical examination, biochemical and microbiological tests. Respiratory diseases were found to exacerbate radiation sickness course. This can be caused by suppression of bone-marrow hemopoiesis, as well as by exacerbation of respiratory diseases, as it follows form radiological reports. In irradiated sheep affected by respiratory diseases the leucocytes content can be at the level of physiological norm due to high original level of white blood cells and reduced betalytic activity of the blood in the initial period of radiation sickness. That is why in order to predict the disease outcome it makes sense to use the lethality index, which is calculated with account of WBC content and β-lytic activity of the blood. The obtained information should be taken into account when analyzing surveillance of livestock animals irradiated as a result of radiological events.

Key words
Sheep, respiratory diseases, lungs radiography, γ-irradiation, radiation sickness, natural resistance, antibacterial activity of blood, β-lytic activity of blood, leucocytes, radiation related death.

References

1. Kartashov P.A., Kirshin V.A., Ilin V.G., Burba L.G., Peskov P.G., Golosnoy V.T. Radiation sickness of farm animals. Moscow, Kolos, 1978. 271 p. (In Russian).

2. Annenkov B.N. Radiation catastrophes: consequences and countermeasures in agriculture. Moscow, Sanepidemia, 2008. 372 p. (In Russian).

3. Gorbatov V.A. Influence of ionizing radiation on the course of infection and the formation of immunity in the erysipelas of pigs and emphysematous carbuncle. Cand. vet. sci. diss. Moscow, 1966. 19 p. (In Russian).

4. Komarov B.A. Effect of X-ray on the course of the infectious process in animals. Proc. of the 2nd scient. conf. "Use of radioisotopes and ionizing radiation in veterinary and animal husbandry". Moscow, 1969, pp. 174-176. (In Russian).

5. Maltsev V.N., Strelnikov V.A. Quantitative regularities of the damaging effect of ionizing radiation on the natural resistance to various infectious agents. ZhMEI – JMEI, 1979, vol. 22, issue 4, pp. 386-394. (In Russian).

6. Yusupov R.R., Sitdikov R.I., Surgutcheva L.M., Sofronychev A.V. The clinic of manifestation of radiation-biological injury of sheep. Veterinarnyj vrach – Veterinary Doctor, 2005, no. 3, pp. 45-48. (In Russian).

8. GOST 33215-2014. The State Standard «Guidelines for accommodation and care of animals. Environment, housing and management. Moscow, Standartinform, 2016. 14 p. (in Russian).

9. Bukharin O.V., Vasilyev N.V. The system of beta-lysine and its role in clinical and experimental medicine. Tomsk, 1977. 189 p. (In Russian).

10. Sarukhanov V.Ya., Isamov N.N. The method for determining the bactericidal activity of blood of agricultural animals: methodical recommendations. Moscow-Obninsk, 2006. 8 p. (In Russian).

11. Sarukhanov V.Ya., Isamov N.N., Epimakhov V.G. Assessment of the effects of external γ-irradiation and an early prognosis of acute radiation sickness in sheep. Veterinarnyy vrach – Veterinary Doctor, 2015, no. 1, pp. 40-43. (In Russian).

12. Pavlishin G.A., Sarapuk I.M. Programmed death of neutrophils in children of early age with community-acquired pneumonia. Zhurnal Grodnenskogo gosudarstvennogo medicinskogo universiteta – Journal of the Grodno State Medical University, 2013, no. 2, pp. 72-74. (In Russian).

13. Mirzoev EН.B. Kruglikov B.P. Evaluation of free radical oxidation of lipids in the blood plasma of irradiated sheep. Radiobiologiya. Radioekologiya – Radiobiology. Radioecology, 1995, vol. 35, issue 2, pp. 189-194. (In Russian).

14. Guryanova V.A., Konyukhov G.V., Troshin E.I. Reaction of lipid peroxidation in testing acute radiation sickness of animals. Proc. of the scientific and production conference “Topical problems of veterinary science and zootechny”, Part 2, Kazan, 2001, pp. 139-140. (In Russian).

7. Mishanin Yu.F., Mishanin M.Yu. Practical veterinary medicine. Rostov-on-Don, 2002. 380 p. (In Russian).

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