Physical, functional and sensory properties of bitter chocolates with incorporation of high nutritional value flours
Luz Quispe-Sanchez1* 
Marilu Mestanza1 
Malluri Goñas1 
Elizabeth Renee Ambler Gill1,2 
Manuel Oliva-Cruz1 
Segundo G. Chavez1- 1Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Chachapoyas, Peru
- 2College of Life Sciences and Agriculture COLSA, University of New Hampshire, Durham, NC, United States
Due to the growing demand for healthy food products, the industry is seeking to incorporate inputs with high nutritional potential to traditional products. The objective of this research was to evaluate the effect of incorporating Lepidium meyenii, Chenopodium pallidicaule, Amaranthus caudatus, Sesamum indicum and Salvia hispanica flours on the physical, chemical, rheological, textural and thermal characteristics, and the degree of sensory acceptance of dark chocolate bars (65% cocoa). To this end, chocolate bars were made with the incorporation of five flours in four doses (1, 2, 3 and 4%), obtaining 20 different formulations compared with a control treatment (without flour addition). It was found that as flour incorporation levels increased, viscosity, antioxidants and particle size of the chocolates increased, but hardness and pH decreased. The addition of the flours also affected the acceptability and microstructure of the chocolate bars. The incorporation of up to 4% of the flours studied improved the degree of acceptance of the chocolates. Consequently, the incorporation of grain flours with high nutritional value can enhance the characteristics of dark chocolates, becoming a technological alternative for the chocolate industry.
Introduction
Among the most popular foods consumed by people of all ages is chocolate. Their consumption is associated with beneficial antioxidant properties that reduce oxidative stress, help prevent cardiovascular disease and improve overall health (1–3). On the other hand, chocolate consumption can improve mood states and cognitive and sensory responses (4); This sensory theory is related to the melting profile in the mouth, and chocolate’s aromatic characteristics that are impacted by the cultivation, harvesting, post-harvesting of cocoa, and processing to obtain chocolate (5).
Global chocolate consumption has been increasing, and the trend toward dark chocolate consumption by individuals has been increasing, as it is considered a healthy, sustainable and high-quality alternative. For example, European countries such as Italy prefer dark chocolates by 46%, followed by chocolate with hazelnut (23%) and chocolates with different additional ingredients (16%) (6).
The EU directive 2000/36/EC of the European Parliament of the European Union has simplified legislation on the use of new ingredients in the manufacture of chocolates, which has opened up the possibility for chocolate manufacturers to develop promising new products. Thus, it is possible to improve not only the nutritional functionality of chocolates and cocoa derivatives, but also to reduce the proportion of cocoa or expensive inputs by substituting them with soluble and insoluble fibers, vitamins, minerals, herbal extracts or other inputs (7). However, in chocolates, the processes of incorporation and/or modification, due to their fatty nature (8), could generate considerable physicochemical changes that modify their sensory properties, affecting consumer perception.
On the other hand, the food industry has implemented rigorous innovation strategies and improved product development as competitive tools (9, 10). This is why considerable changes are being experienced in the cocoa and chocolate industry in response to new consumption trend (11). Such is the case in the United States of America, the consumption of chocolates as functional foods has gained popularity as chocolate is consumed to relax, boost the immune system, and even to improve mood (12, 13).
During the last few years, the trend to consume healthy and safe foods has increased greatly (14). Consumers, in addition to demanding safety and nutrition, seek foods that benefit their health and help prevent diseases (15). In addition to price and preferences, another factor driving food purchases is the food additives incorporated at the time of processing (16, 17). These factors cause manufacturers to take consumer preferences into account when developing and launching new products on the market.
Therefore, the incorporation of new inputs with high nutritional potential can be a good alternative. Maca (Lepidium meyenii), popularly known for improving reproductive health, is a good alternative (18), also acts as an anti-inflammatory (19), anti-fatigue (20), antioxidant (21) and as a stimulant to improve learning and memory processes (22, 23). Cañihua (Chenopodium pallidicaule), due to its high flavonoid content acts as a potent antioxidant (24, 25). Kiwicha (Amaranthus caudatus), has antidiabetic and antihypercholesterolemic effects (26, 27); therefore, it is used as a primary precursor of drugs and food supplements (28–30). Also, pharmacological activities of ajonlojí (Sesamum indicum) have been reported as anticancer, antipyretic, antihypertensive, hepatoprotective and antirheumatoid (31, 32). On the other hand, several researches have focused on studying the properties and applications of chia mucilage (Salvia hispanica) for its high capacity to retain water and modify the texture of foods (33, 34), in addition, due to its high dietary fiber content, it could act as a supplement for the treatment of obesity (35). These foods whose flours are characterized by their high nutritional value (25, 36) could be incorporated into chocolate formulations.
However, aspects such as particle size and flour composition could have a direct impact on the physical, chemical, rheological and even sensory properties of the food (37–39). Además, the refining process to which cocoa nibs are subjected to obtain chocolate could positively affect the availability of nutrients from the meals, as it involves prolonged cell breakdown (37, 40) and they could be encapsulated by the fatty matrix of the product.
To guarantee the quality of chocolates during the production process, aspects such as rheology, texture, physical and chemical properties, which influence sensory characteristics, are monitored (41). In addition, the objective of this research was to study the effect of the incorporation of high nutritional value flours on the physical, functional and sensory properties of dark chocolate.
Methodology
Cocoa and cereals production
The fermented and dried cocoa beans, variety CCN-51 (7.5% moisture), were provided by the Research Institute for Sustainable Development of Ceja de Selva of the UNTRM, obtained from cocoa plantations in the district of Cajaruro, province of Utcubamba, Amazonas region, Peru.
The grains (sesame, cañihua, kiwicha and chia) were purchased at the Central Market in the city of Chachapoyas. In the Cocoa Quality Control Laboratory, here they were removed of impurities, washed and de-saponified with water (kiwicha beans) for 15 min at 75°C. They were then roasted at 110°C for 30 min and ground in a disk mill. The flours obtained were sieved on 850 μm sieve to homogenize. n the case of maca flour, it was purchased, processed and packaged at the same place. Figure 1 shows the seeds and flours used in the experiment, where the cañihua and chia flours had a dark brown color and the maca, kiwuicha and sesame flours had a cream color.
Figure 1. Seeds and flours used in the experiment.
Cocoa paste
Cocoa beans were roasted in a forced convection oven (Venticell Ecoline, Germany) at a temperature of 120°C for 40 min. They were then dehulled in an industrial mill (A&Z, Peru). Once dehulled, the nibs were crushed in a disk mill and refined for 10 h in 3 kg capacity conchers (Premier, India).
Experimental procedure
A 5Ax4B bifactorial experiment was carried out, where A was the type of flour (maca, sesame, cañihua, kiwicha and chia) and B the incorporation dose (1, 2, 3 and 4%). As a result, there were 20 treatments (formulations) and a control treatment (chocolate without flour incorporation). After 10 h of conching-refining of the cocoa nibs, the flours were added, trying to obtain chocolates with 65% cocoa. The refining process continued for 11 more hours. Subsequently, tempering was carried out by raising the temperature to 48°C, then lowering it to 28°C and raising it to 32°C. Bars of 50 g (12 × 5 × 0.5 cm3) and lozenges of 0.75 g (1 × 1 × 0.5 cm3) were molded. The chocolates (tablets) were wrapped in aluminum foil, packed in hermetically sealed PET bags and stored under refrigeration (4–8°C). All treatments were performed in triplicate.
pH
The pH was determined by the AOAC 981.12/90 method (42) with a digital potentiometer (HANNA instruments).
Particle size
Particle size was determined following the procedure described by Ibrahim et al. (43) with some modifications. A solution of melted chocolate in 50% sunflower oil was prepared in a beaker. A drop of the solution was added inside the jaws of the micrometer (Mitutoyo-2017) and closed for reading.
Microstructure
For microstructure analysis, an inverted fluorescence microscope (IX83, Olympus, Tokyo, Japan) with a 40× objective with high resolution polarized light, equipped with a camera (Nikon D810, Tokyo, Japan), was used. For this purpose, melted chocolate at 55°C was placed on a glass slide (44) and micrographs were obtained to observe the distribution and size of the particles in the chocolate.
Hardness
A CTX texture analyzer (AMETEK Brookfield) with TexturePro 1.0.19 software was used, equipped with a 10 kg load cell. The test was performed with a 30°conical probe, a test speed of 1 mm/s and a depth of 0.8 mm. The pre-test speed was 5 mm/s, the post-test speed was 10 mm/s, the data acquisition rate was 10 points per second, and the activation force was 5 g. The hardness in grams of the tablets of the different chocolate formulations was obtained.
Rheological properties
Rheological properties were measured using a modular compact rheometer (Anton Paar, model MCR 302e, Austria), equipped with a CC27 concentric cylinder geometry installed in a Peltier system. The chocolates were pre-melted at 40°C in an oven (Venticell Ecoline, Germany) for 60 min. Rheological measurements were then carried out at 40°C. The measurement cycle started with a preconditioning at 40°C for 60 s, then a shear rate of 5 s-1 was applied for 100 s. Subsequently, the shear rate was increased from 2 to 50 s-1. The data were processed by the equipment software (RheoCompass vs s 1.30) following the Casson model (Equation 1), where σ (Pa) is the shear stress, σ0 is the Casson yield stress (Pa), K1 is the consistency index (Pa. s) and γ (s-1) is the shear rate [Glicerina et al. (45)] and numeral 209 of the International Confectionery Association technical standard (46).
Calorimetric properties
The thermal behavior of chocolates formulated with cereals was measured based on the method of Toker et al. (47), by differential scanning calorimetry (DSC-60 plus, Japan). For this purpose, samples were weighed on an analytical balance (2.5 ± 0.5 mg) and placed in aluminum vials, sealed tightly and subjected to the oven. An empty, sealed aluminum cuvette was used as a blank. Nitrogen gas (25 ml/min) at normal pressure was used. The samples were subjected to a temperature ramp from 0 to 60°C for 20 min to melt all crystals. All measurements were performed in triplicate.
Sensory evaluation
The degree of acceptance of the chocolates obtained with each treatment was evaluated following the procedure of the Spanish Standard UNE-ISO 8587 (48) by 24 untrained judges at the Cocoa and Coffee Quality Control Laboratory. A balanced incomplete block design was employed in two series of seven random distributions taking into account the conditions established in the Standard.
Antioxidant activity
Samples were defatted following the procedure described by Suazo et al. (49). For this purpose, 500 mg of chocolate were weighed and placed in a 15 mL falcon tube, mixed with 3 mL of hexane and shaken at 3,000 rpm for 10 min. The mixture was then centrifuged at 4,830 rpm for 20 min in a centrifuge (PrO-Analytical, Britain) at room temperature. The process was repeated 4 times, then the supernatant was removed and the defatted chocolate powder was recovered. The defatted powders were left for 24 h in a fume hood to remove the solvent in the dark.
Next, methanolic extracts were obtained using the method described by Jonfia-Essien, West, Alderson and Tucker (50) with some modifications. For this, 0.1 g of defatted sample was taken, mixed with 10 ml of 80% methanol solution, shaken for 15 min in a magnetic stirrer (Velp. Scientifica) at room temperature. Then, it was centrifuged at 4,830 rpm for 30 min, filtered on filter paper (Whatman N° 40–2.5 μm) and the supernatant was stored in amber bottles with lids at −24°C until further analysis.
DPPH
DPPH analysis (% free radical uptake) was performed following the method reported by Brand-Williams et al. (51), which indicates that DPPH must first be prepared in 20 mg/L methanol. Then, a methanolic solution of the sample (Solution A1) to be analyzed was prepared at a concentration of 300 μg/ml, from which 0.75 ml was taken to prepare the sample blank + 1.5 ml of methanol (A3) and 0.75 ml to prepare the sample + 1.5 ml of DPPH methanolic solution (A2), left for 5 min in the dark and the absorbance was read at 517 nm in the spectrophotometer (T 9200 PEAK Instruments, United States). Finally, with the values of the absorbances obtained, the DPPH was determined according to the formula of Equation 2, where A0: Absorbance of the DPPH solution, AS: Absorbance of methanol, AT: Absorbance of the sample.
Results and discussion
Particle size, sensory acceptability, pH, hardness and antioxidant activity
The particle size of the chocolate bars is different according to the type of flour incorporated into the formulation (Table 1). The higher degrees of flour incorporation (3 and 4%) allowed obtaining chocolates with lower hardness (from 6,716 to 3,677 g), agreeing with previous works that showed that the inclusion of other foods in the formulations affects the rheological and visual properties of the chocolates (52).
Table 1. Results of particle size, sensory acceptance, hydrogen potential, hardness and antioxidant activity of dark chocolates enriched with 5 types of high-nutritional value flours, in different percentages.
Similarly, the pH of the chocolates was slightly modified for all treatments, decreasing to 5.21 for sesame flour; which may be due to the chemical composition of the flour (starch, fatty acids) (53).
The dark chocolate formulations with the addition of flours had particle sizes between 20 and 144 μm, while the control chocolate (without flour) had a particle size of 22.7 μm. Sesame, cañigua and chia flours had the least effect on the particle size of the chocolate (< 56 μm). In contrast, when kiwicha and maca flours were incorporated, the particle size increased considerably, up to 96 and 144 μm, respectively (Table 1). Article size in chocolates is very important in sensory perception; in fact, a chocolate could be perceived as gritty when the particles are larger than 25 μm (44). Table 1 also shows that the incorporation of flours improves the degree of sensory acceptability of dark chocolates, since all treatments scored higher than the control treatment (p < 0.05), highlighting the treatments with incorporation of cañigua and chia flour in all degrees of incorporation, which reached acceptability scores between 2.58 and 3.13. Previous studies have already demonstrated that chocolate flavor is influenced by the composition of the added inputs (54), this theory can support the different degrees of acceptability of the chocolates obtained in this research.
The incorporation of the flours increased the antioxidant capacity of the chocolates. With the addition of the flours, chocolates with higher antioxidative activity were obtained compared to the control treatment which had 82.5 mmol/L TE (Table 2). Evidently, there is a direct effect on the content of antioxidant compounds by the addition of the flours. In addition, the incorporated antioxidant compounds can improve the nutritional and funtional value, the chemical contribution and can stabilize the oxidation of chocolate fats during storage (55).
Table 2. Rheological properties of dark chocolates enriched with high nutritional value flours.
On the other hand, darker flours (cañihua and chia) increased the antioxidant capacity of chocolates to a greater extent than lighter flours (maca and sesame), which may be due to the fact that foods with more color contain more phenolic compounds and greater antioxidative activity (56).
Therefore, the chemical composition of flours can influence the physical, chemical and sensory properties of chocolates. For example, the inclusion of lentil flour has been studied and depending on the matrix in which it is incorporated, it modifies the rheological, textural and sensory acceptance properties of the final product (57, 58).
As shown in Table 1, all flours modified the characteristics of the chocolates with respect to the control. Flour additions increased particle size, but all treatments significantly improved (p < 0.05) the degree of sensory acceptance, increased pH and antioxidant activity. On the other hand, doses higher than 2% reduced the hardness of the chocolate bars. Consequently, based on the degree of acceptance and antioxidant activity with 0.05 of statistical significance, the best alternatives would be: maca 4%, sesame 2%, cañigua 1–4%, kiwicha 1% and chia 1–4%.
Microstructure
As the flour content increases, the microstructure of the chocolates is affected (Figure 2). The incorporation of up to 2% of chia and cañigua flours makes it possible to obtain chocolates with a more uniform structure; however, levels higher than 2% increase the granulometry of the final product. Por tanto, kiwicha, sesame and maca flours affect the microstructural aspect of the chocolates. Previous studies showed that the incorporation of flours affects the microstructure of chocolates (8), interfering in the fat classification and crystallization process (59).
Figure 2. Micrograph of dark chocolates enriched with high-nutritional value flours in different percentages.
The effects found in this study are variable due to the nature of each source. For example, chia flour, in addition to containing fats, is an important source of gummy elements (60) that can help in the structure of chocolate bars. As it was evidenced that the addition of chia flour in bread increases the moisture and lipid content (61) because these elements represent more than 30% of its composition (62, 63).
Textural profile of chocolates with flours
Table 1 shows that the addition of different types of flour in the formulations also modifies the hardness of the chocolates (from 2,587 to 6,716 g). This has already been demonstrated in previous studies (64). However, high levels of flour (more than 3%) reduce the hardness of chocolates, which could be due to the fact that flours modify the viscosity of the paste (during the refining process) and interfere in the fat sorting and crystallization process (59). Texture profiles are presented in Figure 3, where chocolate bars with higher contents of maca, sesame, cañigua, kiwicha, and chia flour took less time to fracture (5 min approx.) and unlike the other flours, they offered resistance until the course was completed (Figure 3E), which illustrates the action of the flours of these seeds on the physical property of the food (65, 66).
Figure 3. Typical texture curves of dark chocolates with the addition of flours. (A) Maca, (B) ajonjolí, (C) kiwicha, (D) cañihua, (E) chia, and (F) with no flours.
Rheological properties
As previous studies have shown, the rheological properties of chocolate are affected by the incorporation of other products and, particle size is inversely correlated with the firmness, consistency, cohesiveness, viscosity and hardness of chocolate bars (67, 73). Smaller particles have also been found to favor higher dispersibility of chocolate ingredients, because they occupy the spaces between larger particles, leading to a lubricating effect with a consequent reduction in viscosity (68). Tighter results could be obtained by conditioning other process factors such as refining, time, process temperature and tempering of the chocolate bars.
In this study, the rheological properties of chocolates with incorporated cereal flour were studied at steady state and plastic viscosity. Dark chocolates have a complex rheological behavior (non-Newtonian liquid) showing an apparent yield strength and plastic viscosity that depends on the manufacturing process (69). The incorporated flours modified the rheological properties of the chocolate bars to different degrees; in fact, with 4% incorporation of maca, cañihua and chia, chocolates with viscosities higher than 3.5 Pa.s were obtained, and with sesame and kiwicha, plastic viscosities lower than 2.93 Pa.s were obtained (Table 2).
The determination of the rheological properties of chocolate is crucial to develop high quality products with a well-defined texture (70). As shown in Table 2, the incorporation of flours increases the viscosity of the chocolates, with cañihua and chia flours having the greatest effect on this property. Bourré et al. (71) demonstrated that there is greater starch damage with intense flour milling (< 500 μm), increasing water absorption capacity and viscosity.
Calorimetric properties
Figure 4 shows the thermograms obtained for each treatment. Although the enthalpy varies according to the type and percentage of flour added, we observed that when the amount of flour in the formulations is increased, the melting temperature of the chocolate also increases. The values found ranged from 31 to 37°C for all treatments, which is close to the optimal range (31.5–32°C) described by other authors (8). Indeed, tempering conditions are fundamental and should be taken into account in future studies, addressing tempering models (72).
Figure 4. Typical differential scanning calorimetry curves of dark chocolates with the addition of flours. (A) Maca, (B) ajonjolí, (C) kiwicha, (D) cañihua, (E) chia, and (F) with no flours.
Conclusion
The results of this study revealed that the incorporation of flours has considerable potential as an alternative to synthetic additives in chocolates by improving their antioxidant capacity. Indeed, the incorporation of flours significantly increased the antioxidant activity of the chocolate samples; however, there was a slight difference in the rheological and melting properties. The addition of up to 4% of chia and cañihua flours to chocolates resulted in chocolates with higher sensory acceptability than chocolates with sesame, kiwicha, maca and even the control treatment. Our finding will contribute to continue developing new products, improving the rheological and sensory properties of chocolate.
Data availability statement
The original contributions presented in this study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.
Author contributions
MO-C and SC: conceptualization. LQ-S and MM: methodology. SC, LQ-S, and MM: formal analysis. MO-C, ERAG, and MG: research and writing—preparing the original draft. LQ-S, MM, MG, ERAG, and SC: writing—revision and editing. MO-C, ERAG, SC, and MG: funding acquisition. All authors contributed to the article and approved the submitted version.
Funding
The authors acknowledge funding from the Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica (FONDECYT) through the subproject “Metagenomic analysis and chromatographic techniques for obtaining a starter culture to improve the quality of fine aroma native cocoa chocolate in the North Eastern zone of Peru” - METACACAO, under Contract No. 008-2020-FONDECYT-BM, and the Project with Contract No. 026-2016 Research Circle for Innovation and the strengthening of the value chain of fine aroma native cocoa in the North Eastern zone of Peru-CINCACAO, the support and execution of this project by the Research Institute for Sustainable Development of Ceja de Selva (INDES-CES).
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
1. Aktar T, Chen J, Ettelaie R, Holmes M, Henson B. Human roughness perception and possible factors effecting roughness sensation. J Texture Stud. (2017) 48:181–92. doi: 10.1111/jtxs.12245
2. Cooper KA, Donovan JL, Waterhouse AI, Williamson G. Cocoa and health: a decade of research. Br J Nutr. (2008) 99:1–11. doi: 10.1017/S0007114507795296
3. Hooper L, Kay C, Abdelhamid A, Kroon PA, Cohn JS, Rimm EB, et al. Effects of chocolate, cocoa, and flavan-3-ols on cardiovascular health: a systematic review and meta-analysis of randomized trials. Am J Clin Nutr. (2012) 95:740–51. doi: 10.3945/ajcn.111.023457
4. Maleki S, Amiri Aghdaie SF, Shahin A, Ansari A. Investigating the relationship among the Kansei-based design of chocolate packaging, consumer perception, and willingness to buy. J Mark Commun. (2020) 26:836–55. doi: 10.1080/13527266.2019.1590855
5. Braga SCGN, Oliveira LF, Hashimoto JC, Gama MR, Efraim P, Poppi RJ, et al. Study of volatile profile in cocoa nibs, cocoa liquor and chocolate on production process using GC × GC-QMS. Microchem J. (2018) 141:353–61. doi: 10.1016/j.microc.2018.05.042
6. Silva AR, de A, Bioto AS, Efraim P, Queiroz G, de C. Impact of sustainability labeling in the perception of sensory quality and purchase intention of chocolate consumers. J Cleaner Prod. (2017) 141:11–21. doi: 10.1016/j.jclepro.2016.09.024
7. Bolenz S, Amtsberg K, Schäpe R. The broader usage of sugars and fillers in milk chocolate made possible by the new EC cocoa directive. Int J Food Sci Technol. (2006) 41:45–55. doi: 10.1111/j.1365-2621.2005.01023.x
8. Devos N, Reyman D, Sanchez-Cortés S. Chocolate composition and its crystallization process: a multidisciplinary analysis. Food Chem. (2021) 342:128301. doi: 10.1016/j.foodchem.2020.128301
9. De Pelsmaeker S, Gellynck X, Delbaere C, Declercq N, Dewettinck K. Consumer-driven product development and improvement combined with sensory analysis: a case-study for European filled chocolates. Food Qual Prefer. (2015) 41:20–9. doi: 10.1016/j.foodqual.2014.10.009
10. Morato PN, Rodrigues JB, Moura CS, e Silva FGD, Esmerino EA, Cruz AG, et al. Omega-3 enriched chocolate milk: a functional drink to improve health during exhaustive exercise. J Func Foods. (2015) 14:676–83. doi: 10.1016/j.jff.2015.02.034
11. Belščak-Cvitanović A, Komes D, Dujmović M, Karlović S, Biškić M, Brnčić M, et al. Physical, bioactive and sensory quality parameters of reduced sugar chocolates formulated with natural sweeteners as sucrose alternatives. Food Chem. (2015) 167:61–70. doi: 10.1016/j.foodchem.2014.06.064
12. Barry Callebaut. Barry Callebaut International Consumer Survey Shows: Chocolate Lovers Want Functional Chocolate With Proven Health Benefits!. Barry callebaut. (2008). Available online at: https://www.barry-callebaut.com/en/group/media/news-stories/barry-callebaut-international-consumer-survey-shows-chocolate-lovers-want (accessed July 8, 2022).
13. Saka EK. The Design Of Packaging Graphics For The Expansion Of Ghanaian Chocolate Products. Ph.D. thesis. Ames, IA: Iowa State University (2011). doi: 10.31274/etd-180810-2548
14. Julio LM, Copado CN, Crespo R, Diehl BWK, Ixtaina VY, Tomás MC. Design of microparticles of chia seed oil by using the electrostatic layer-by-layer deposition technique. Powder Technol. (2019) 345:750–7. doi: 10.1016/j.powtec.2019.01.047
15. Marsanasco M, Calabró V, Piotrkowski B, Chiaramoni NS, del V, Alonso S. Fortification of chocolate milk with omega-3, omega-6, and vitamins E and C by using liposomes. Eur J Lipid Sci Technol. (2016) 118:1271–81. doi: 10.1002/ejlt.201400663
16. Harwood ML. A Novel Method To Assess Consumer Acceptance of Bitterness in Chocolate Products. US: Pennsylvania State University the Graduate School College of Agricultural Sciences (2013).
17. Konar N, Toker OS, Oba S, Sagdic O. Improving functionality of chocolate: a review on probiotic, prebiotic, and/or synbiotic characteristics. Trends Food Sci Technol. (2016) 49:35–44. doi: 10.1016/j.tifs.2016.01.002
18. Beharry S, Heinrich M. Is the hype around the reproductive health claims of maca (Lepidium meyenii Walp.) justified? J Ethnopharmacol. (2018) 211:126–70. doi: 10.1016/j.jep.2017.08.003
19. Bai N, He K, Roller M, Lai CS, Bai L, Pan MH. Flavonolignans and other constituents from lepidium meyenii with activities in anti-inflammation and human cancer cell lines. J Agric Food Chem. (2015) 63:2458–63. doi: 10.1021/acs.jafc.5b00219
20. Tang W, Jin L, Xie L, Huang J, Wang N, Chu B, et al. Structural characterization and antifatigue effect in vivo of maca (Lepidium meyenii Walp) polysaccharide. J Food Sci. (2017) 82:757–64. doi: 10.1111/1750-3841.13619
21. Korkmaz S. Antioxidants in maca (Lepidium meyenii) as a supplement in nutrition. In: E Shalaby editor. Antioxidants in Foods and Its Applications. London: IntechOpen (2018). p. 138–54.
22. Carvalho FV, Ribeiro PR. Structural diversity, biosynthetic aspects, and LC-HRMS data compilation for the identification of bioactive compounds of Lepidium meyenii. Food Res Int. (2019) 125:108615. doi: 10.1016/j.foodres.2019.108615
23. Carvalho FV, Ferraz CG, Ribeiro PR. Pharmacological activities of the nutraceutical plant Lepidium meyenii: a critical review. J Food Chem Nanotechnol. (2020) 6:107–16. doi: 10.17756/jfcn.2020-091
24. Kokanova-Nedialkova Z, Nedialkov P, Kondeva-Burdina M, Simeonova R, Tzankova V, Aluani D. Chenopodium bonus-henricus L. – A source of hepatoprotective flavonoids. Fitoterapia (2017) 118:13–20. doi: 10.1016/j.fitote.2017.02.001
25. Repo-Carrasco-Valencia R, Hellström JK, Pihlava JM, Mattila PH. Flavonoids and other phenolic compounds in andean indigenous grains: Quinoa (Chenopodium quinoa), kañiwa (Chenopodium pallidicaule) and kiwicha (Amaranthus caudatus). Food Chem. (2010) 120:128–33. doi: 10.1016/j.foodchem.2009.09.087
26. Girija K, Lakshman K, Udaya C, Sabhya Sachi G, Divya T. Anti-diabetic and anti-cholesterolemic activity of methanol extracts of three species of Amaranthus. Asian Pacific J Trop Biomed. (2011) 1:133–8. doi: 10.1016/S2221-1691(11)60011-7
27. Martinez-Lopez A, Millan-Linares MC, Rodriguez-Martin NM, Millan F, Montserrat-de la Paz S. Nutraceutical value of kiwicha (Amaranthus caudatus L.). J Funct Foods. (2020) 65:103735. doi: 10.1016/j.jff.2019.103735
28. Jimoh MO, Afolayan AJ, Lewu FB. Antioxidant and phytochemical activities of Amaranthus caudatus L. harvested from different soils at various growth stages. Sci Rep. (2019) 9:1–14. doi: 10.1038/s41598-019-49276-w
29. Jimoh MO, Afolayan AJ, Lewu FB. Nutrients and antinutrient constituents of Amaranthus caudatus L. Cultivated on different soils. Saudi J Biol Sci. (2020) 27:3570–80. doi: 10.1016/j.sjbs.2020.07.029
30. Man S, Pãucean A, Muste S, Chi? M-S, Pop A, Ianoş I-DC. Assessment of amaranth flour utilization in cookies production and quality. J Agroalimentary Process Technol. (2017) 23:97–103.
31. Mili A, Das S, Nandakumar K, Lobo R. A comprehensive review on Sesamum indicum L: botanical, ethnopharmacological, phytochemical, and pharmacological aspects. J Ethnopharmacol. (2021) 281:114503. doi: 10.1016/j.jep.2021.114503
32. Ruckmani A, Meti V, Vijayashree R, Arunkumar R, Konda VR, Prabhu L, et al. Anti-rheumatoid activity of ethanolic extract of Sesamum indicum seed extract in Freund’s complete adjuvant induced arthritis in Wistar albino rats. J Tradit Complement Med. (2018) 8:377–86. doi: 10.1016/j.jtcme.2017.06.003
33. Campo C, Stefer M, Bernard L, Hengy S, Boeglen H, Paillot JM. Antenna weighting system for a uniform linear array based on software defined radio. Proceedings of the Mediterranean Microwave Symposium, 2017-Novem. (2018). p. 1–4. doi: 10.1109/MMS.2017.8497138
34. Timilsena YP, Adhikari R, Kasapis S, Adhikari B. Molecular and functional characteristics of purified gum from Australian chia seeds. Carbohydr Polym. (2016) 136:128–36. doi: 10.1016/j.carbpol.2015.09.035
35. Vuksan V, Jenkins AL, Brissette C, Choleva L, Jovanovski E, Gibbs AL, et al. Salba-chia (Salvia hispanica L.) in the treatment of overweight and obese patients with type 2 diabetes: a double-blind randomized controlled trial. Nutr Metab Cardiovasc Dis. (2017) 27:138–46. doi: 10.1016/j.numecd.2016.11.124
36. Alarcón-García MA, Perez-Alvarez JA, López-Vargas JH, Pagán-Moreno MJ. Techno-functional properties of new andean ingredients: maca (Lepidium meyenii) and amaranth (Amaranthus caudatus). Proceedings. (2020) 70:74. doi: 10.3390/foods_2020-07744
37. Boukid F, Vittadini E, Lusuardi F, Ganino T, Carini E, Morreale F, et al. Does cell wall integrity in legumes flours modulate physiochemical quality and in vitro starch hydrolysis of gluten-free bread? J Func Foods. (2019) 59:110–8. doi: 10.1016/j.jff.2019.05.034
38. Pellegrini N, Vittadini E, Fogliano V. Designing food structure to slow down digestion in starch-rich products. Curr Opin Food Sci. (2020) 32:50–7. doi: 10.1016/j.cofs.2020.01.010
39. Rovalino-Córdova AM, Fogliano V, Capuano E. The effect of cell wall encapsulation on macronutrients digestion: a case study in kidney beans. Food Chem. (2019) 286:557–66. doi: 10.1016/j.foodchem.2019.02.057
40. Joshi M, Timilsena Y, Adhikari B. Global production, processing and utilization of lentil: a review. J Integrat Agric. (2017) 16:2898–913. doi: 10.1016/S2095-3119(17)61793-3
41. Gonçalves EV, da Silva Lannes SC. Chocolate rheology. Sci Food Technol. (2010) 30:845–51. doi: 10.1590/S0101-20612010000400002
42. AOAC. Official Methods of Analysis of AOAC International. 17th ed. Washington DC: Association of Official Analytical Chemist (1990).
43. Ibrahim SF, Ezzati NS, Dalek M, Firdaus QA, Raffie M, Ain MRF. Quantification of physicochemical and microstructure properties of dark chocolate incorporated with palm sugar and dates as alternative sweetener. Material Today. (2020) 31:366–71. doi: 10.1016/j.matpr.2020.06.235
44. Afoakwa EO, Paterson A, Fowler M, Vieira J. Microstructure and mechanical properties related to particle size distribution and composition in dark chocolate. Int J Food Sci Technol. (2009) 44:111–9. doi: 10.1111/j.1365-2621.2007.01677.x
45. Glicerina V, Balestra F, Rosa MD, Romani S. Rheological, textural and calorimetric modifications of dark chocolate during process. J Food Engin. (2013) 119:173–9. doi: 10.1016/j.jfoodeng.2013.05.012
47. Toker OS, Palabiyik I, Konar N. Chocolate quality and conching. Trends Food Sci Technol. (2019) 91:446–53. doi: 10.1016/j.tifs.2019.07.047
49. Suazo Y, Davidov-Pardo G, Arozarena I. Effect of fermentation and roasting on the phenolic concentration and antioxidant activity of cocoa from nicaragua. J Food Qual. (2014) 37:50–6. doi: 10.1111/jfq.12070
50. Jonfia-Essien WA, West G, Alderson PG, Tucker G. Phenolic content and antioxidant capacity of hybrid variety cocoa beans. Food Chem. (2008) 108:1155–9. doi: 10.1016/j.foodchem.2007.12.001
51. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol. (1995) 28:25–30. doi: 10.1016/S0023-6438(95)80008-5
52. Afoakwa EO, Paterson A, Fowler M. Effects of particle size distribution and composition on rheological properties of dark chocolate. Eur Food Res Technol. (2008) 226:1259–68. doi: 10.1007/s00217-007-0652-6
53. Spada FP, Mandro GF, da Matta MD, Canniatti-Brazaca SG. Functional properties and sensory aroma of roasted jackfruit seed flours compared to cocoa and commercial chocolate powder. Food Biosci. (2020) 37:100683. doi: 10.1016/j.fbio.2020.100683
54. Yao G, Gao P, Zhang W. Complete genome sequence of probiotic Bacillus coagulans HM-08: a potential lactic acid producer. J Biotechnol. (2016) 228:71–2. doi: 10.1016/j.jbiotec.2016.04.045
55. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol. (1995) 28:25–30.
56. Rodríguez Madrera R, Campa Negrillo A, Suárez Valles B, Ferreira Fernández JJ. Phenolic content and antioxidant activity in seeds of common bean (Phaseolus vulgaris L.). Foods (2021) 10:864. doi: 10.3390/foods1004086465
57. Luo S, Chan E, Masatcioglu MT, Erkinbaev C, Paliwal J, Koksel F. Effects of extrusion conditions and nitrogen injection on physical, mechanical, and microstructural properties of red lentil puffed snacks. Food Bioprod Process. (2020) 121:143–53. doi: 10.1016/j.fbp.2020.02.002
58. Portman D, Blanchard C, Maharjan P, McDonald LS, Mawson J, Naiker M, et al. Blending studies using wheat and lentil cotyledon flour—Effects on rheology and bread quality. Cereal Chem. (2018) 95:849–60. doi: 10.1002/cche.10103
59. Lapčíková B, Lapčík L, Salek R, Valenta T, Lorencová E, Vašina M. Physical characterization of the milk chocolate using whey powder. LWT. (2022) 154:112669. doi: 10.1016/j.lwt.2021.112669
60. Segura-Campos MR, Ciau-Solís N, Rosado-Rubio G, Chel-Guerrero L, Betancur-Ancona D. Chemical and functional properties of chia seed (Salvia hispanica L.) gum. Int J Food Sci. (2014) 2014:241053. doi: 10.1155/2014/241053
61. Huerta K, Soquetta M, Alves J, Stefanello R, Kubota E, Rosa CS. Effect of flour chia (Salvia hispanica L.) as a partial substitute gum in gluten free breads. Int Food Res J. (2018) 25:755–61.
62. Fernandes SS, Tonato D, Mazutti MA, de Abreu BR, Cabrera DDC, D’Oca CDRM, et al. Yield and quality of chia oil extracted via different methods. J Food Engin. (2019) 262:200–8. doi: 10.1016/j.jfoodeng.2019.06.019
63. Fernandes SS, Salas-Mellado MDLM. Addition of chia seed mucilage for reduction of fat content in bread and cakes. Food Chem. (2017) 227:237–44. doi: 10.1016/j.foodchem.2017.01.075
64. Hosseini AF, Mazaheri-Tehrani M, Yeganehzad S, Razavi SMA. Investigation of the rheological, thermal, sensory properties, and particle size distribution of sesame paste white compound chocolate as influenced by the soy flour and emulsifier levels. Int J Food Eng. (2020) 16:20190272. doi: 10.1515/ijfe-2019-0272
65. Moreno C. Desarrollo y Evaluación de un Chocolate Funcional Incorporando dos Tipos de Extracto a dos Concentraciones de Dulcamara (Solanum dulcamara L.) [Universidad]. (2009). Available online at: http://hdl.handle.net/11036/289
66. Ceballos AM, Giraldo GI, Orrego CE. Effect of freezing rate on quality parameters of freeze dried soursop fruit pulp. J Food Eng. (2012) 111:360–5. doi: 10.1016/j.jfoodeng.2012.02.010
67. Oba S, Toker OS, Palabiyik I, Konar N, Goktas H, Cukur Y, et al. Rheological and melting properties of sucrose-free dark chocolate. Int J Food Proper. (2017) 20:2096–106. doi: 10.1080/10942912.2017.1362652
68. Coutinho NM, Silveira MR, Pimentel TC, Freitas MQ, Moraes J, Fernandes LM, et al. Chocolate milk drink processed by cold plasma technology: physical characteristics, thermal behavior and microstructure. LWT. (2019) 102:324–9. doi: 10.1016/j.lwt.2018.12.055
69. Afoakwa EO, Paterson A, Fowler M. Factors influencing rheological and textural qualities in chocolate – a review. Trends Food Sci Technol. (2007) 18:290–8. doi: 10.1016/j.tifs.2007.02.002
71. Bourré L, Frohlich P, Young G, Borsuk Y, Sopiwnyk E, Sarkar A, et al. Influence of particle size on flour and baking properties of yellow pea, navy bean, and red lentil flours. Cereal Chem. (2019) 96:655–67. doi: 10.1002/cche.10161
Keywords: cañihua, kiwicha, calorimetry, rheology, antioxidant activity
Citation: Quispe-Sanchez L, Mestanza M, Goñas M, Gill ERA, Oliva-Cruz M and Chavez SG (2022) Physical, functional and sensory properties of bitter chocolates with incorporation of high nutritional value flours. Front. Nutr. 9:990887. doi: 10.3389/fnut.2022.990887
Received: 10 July 2022; Accepted: 29 August 2022;
Published: 20 September 2022.
Edited by:
Hayriye Sebnem Harsa, Izmir Institute of Technology, TurkeyReviewed by:
Linhai Wang, Oil Crops Research Institute (CAAS), ChinaDanar Praseptiangga, Sebelas Maret University, Indonesia
Copyright © 2022 Quispe-Sanchez, Mestanza, Goñas, Gill, Oliva-Cruz and Chavez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Luz Quispe-Sanchez, luz.quispe.epg@untrm.edu.pe