DNA Examination of Polymorphism on Two Lines of the Yakut Breed Stallions using the RAPD-PCR Method
L. G. Kozlova1, V. G. Osipov2, N. A. Nikolaeva3, A. D. Reshetnikov4, A. I. Barashkova5, Y. L. Shadrina6
1L.G. Kozlova, FRC YaSC SB RAS, M.G. Safronov Yakut scientific research institute of agriculture, Yakutsk, Russia.
2V.G. Osipov, FRC YaSC SB RAS, M.G. Safronov Yakut scientific research institute of agriculture, Yakutsk, Russia.
3N.A. Nikolaeva, FRC YaSC SB RAS, M.G. Safronov Yakut scientific research institute of agriculture, Yakutsk, Russia.
4A.D. Reshetnikov, FRC YaSC SB RAS, M.G. Safronov Yakut scientific research institute of agriculture, Yakutsk, Russia.
5A.I. Barashkova, FRC YaSC SB RAS, M.G. Safronov Yakut scientific research institute of agriculture, Yakutsk, Russia.
6Y.L. Shadrina, FRC YaSC SB RAS, M.G. Safronov Yakut scientific research institute of agriculture, Yakutsk, Russia.
Manuscript received on September 23, 2019. | Revised Manuscript received on October 15, 2019. | Manuscript published on October 30, 2019. | PP: 6634-6638 | Volume-9 Issue-1, October 2019 | Retrieval Number: A1877109119/2019©BEIESP | DOI: 10.35940/ijeat.A1877.109119
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© The Authors. Blue Eyes Intelligence Engineering and Sciences Publication (BEIESP). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: Herd and beef horse breeding are traditional and profitable livestock industries in the Republic of Sakha (Yakutia). Three breeds are propagated in the republic: Yakut, Prilensk and Megezhek. Objective: to evaluate the genetic homogeneity of equine micro-populations in order to determine the degree of their genetic similarity. A DNA isolated from hair follicles of 2 horse lines have been tested using 18 primers. According to obtained data, the primers OPA 07, OPA 09, OPA 10, OPA 18 and ODS 18 are more polymorphic than other 13 primers. Most of the primers amplify fragments from 350 to 1,000 bp in size. As an exception the sequence of primer OPA 02 is absent in 2 lines of horses. Coincidently, PRA 06 primer fragment of 1200 bp presents only in the mare line of the stallion named Kuobakh. There is a uniform distribution of fragments over the entire range of lengths in the remaining samples of 2 stallion lines. Random Amplification of Polymorphic DNA (PARD) analysis can serve as a kind of express method a method of genetic polymorphism, which is especially relevant for poorly studied animal populations, as well as a source of unique locus-specific markers.
Keywords: Breed, DNA, horses, polymorphism, primers, RAPD-PCR.