Abstract
Neonatal diarrhea is a complex infectious disease caused by the interaction of adverse environmental conditions, deficient immune status of neonates and infection of enteropathogens. A variety of microorganisms can cause diarrhea in alpaca neonates, including bacteria, viruses and parasites. In the 1980s, isolation of Escherichia coli strains capable of inducing fluid accumulation in the intestinal loop assay in alpaca and llama neonates was described. However, virulence factors of E.coli associated with neonatal diarrhea in South American camelids (SAC) have been poorly characterised. In this study, 94 E.coli isolates from 94 crias of alpaca (1 per animal) with diarrhea were analysed for the presence of virulence factor genes of diarrheagenic E.coli by PCR. The investigated genes were verotoxin (vt1 and vt2), intimin (eae), and bundle- forming pili (bfp) genes. The animals belonged to six different herds of the Andean region of Cuzco (Peru) and were between two and nine weeks old. Two categories of diarrheagenic E.coli strains were detected: Enteropathogenic E.coli (EPEC), and Verotoxin-producing E.coli (VTEC) strains. Out of the 94 analysed strains 2.13% (2/94) were EPEC (eaeandbfp), 10.64% (10/94) were atypical EPEC (eae but bfp negative), and 11.7% (11/94) were VTEC. Only 3 of the VTEC strains (3/11) were eae positive: one eae vt2 and two eae vt1. All of the other VTEC strains (8/11) were only vt1. Infection with diarrheagenic E.coli strains was detected in all the herds. The results indicate that these pathogenic strains are circulating among domestic SAC, probably at high rates, in the Andean region of Peru. Therefore, EPEC and VTEC strains may be a leading cause of diarrhea in these animals. In addition, these pathogens are a matter for public health. Preventive measures based on vaccination and improvement of hygiene conditions are essential to reduce the impact of neonatal diarrhea in SAC health and mortality in the Andean region of Peru.
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References
Blanco J., M. Blanco, J.E. Blanco, A. Mora, M.P. Alonso, E.A. González and M.I. Bernárdez, 2001. Epidemiology of verocytotoxigenic Escherichia coli (VTEC) in ruminants. In: G. Duffy, P. Garvey and A. McDowell (eds.) Verocytotoxigenic E. coli.. Food and Nutrition Press, Trumbull, CT, USA, pp. 113–148.
Blanco M., J.E. Blanco, A. Mora, G. Dahbi, M.P. Alonso, E.A. González, M.I. Bernárdez and J. Blanco, 2004. Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from cattle in Spain and identification of a new intimin variant gene (eae-xi). Journal of Clinical Microbiology, 42: 645–51.
Bustinza AV, P.J. Burfening and R.L. Blackwell, 1988. Factors affecting survival in young alpacas (Lamapacos). Journal of Animal Science, 66: 1139–1143.
Cid D., J.A. Ruiz-Santa-Quiteria, I. Marín, R. Sanz, J.A. Orden, R. Amils and R. de la Fuente, 2001. Association between intimin (eae) and EspB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kids. Microbiology, 147: 2341–5233.
Cordeiro A., M. Blanco, W. Huanca, A. Mora, A. Herrera, B. Puentes, E. Quina, C. Lopez, R. Mamani, J.E. Blanco and J. Blanco, 2009. Determinatión de serotipos y genes de virulencia de Escherichia coli verotoxigénicos (ECVT), en coprocultivos de alpacas (Vicugnapacos) clínicamente sanas, procedentes de puna húmeda del Perú. XXI Meeting of the Latin American Association of Animal Production (ALPA). 18–23 October.
Fernández Baca S., 2005. Situatión actual de los camélidos sudamericanos en Peru. Proyecto de Cooperatión Técnica en apoyo de la crianza y aprovechamiento de los Camelidos Sudamericanos en la Region Andina TCP/RLA/2914. Food and Agriculture Organisation (FAO).
Foster A.P., A. Otter, A.M. Barlow, G.R. Pearson, M.J. Woodward and R.J. Higgins, 2008. Naturally occurring intestinal lesions in three alpacas (Vicugna pacos) caused by attaching and effacing Escherichia coli. Veterinary Record, 162(10): 318–320.
Gyles C.L., 2007. Shiga toxin-producing Escherichia coli: An overview. Journal of Animal Science, 85: E45-E62.
Kaper J.B., J.P. Nataro and H.L. Mobley, 2004. Pathogenic Escherichia coli. Nature reviews. Microbiology, 2: 123–140.
Karmali M.A., 2004. Shiga toxin-producing Escherichia coli. Molecular Biotechnology, 26: 117–122
Leotta G.A., N. Deza, J. Origlia, C. Toma, I. Chinen, E. Miliwebsky, S. Iyoda, S. Sosa-Estani and M. Rivas, 2006. Short communication. Veterinary Microbiology, 118: 151–157.
Luna L., 2009. Genotipificación de cepas de Escherichia coli aislados de crias de alpacas con diarreas. Thesis, Faculty of Veterinary Medicine, Univesity of San Marcos, Lima, Peru, p. 70.
Mainil J.G. and G. Daube, 2005. Verotoxigenic Escherichia coli from animals, humans and foods: who’s who? Journal of Applied Microbiology, 98:1332–1344.
Martín Espada C., C.E. Pinto Jiménez and D. Cid Vázquez, 2010. South american camelids: health status of their cria. Revista Complutense de Ciencias Veterinarias. 4: 37–50.
Mercado E.C., S.M. Rodríguez, A.M. Elizondo, G. Marcoppido and V. Parreño, 2004. Isolation of shiga toxin-producing Escherichia coli from a South American camelid (Lama guanicoe) with diarrhea. Journal of Clinical Microbiology, 42(10): 4809–11.
Nataro P.J. and J.B. Kaper, 1998. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11: 142–201.
Ochoa T.J., F. Barletta, C. Contreras and E. Mercado, 2008. New insights into the epidemiology of enteropathogenic Escherichia coli infection. Transactions of the Royal Society of Tropical Medicine and Hygiene, 102: 852–856.
Pritchard G.C., R. Smith, J. Ellis-Iversen, T. Cheasty and G.A. Willshaw, 2009. Verocytotoxigenic Escherichia coli O157 in animals on public amenity premises in England and Wales, 1997 to 2007. Veterinary Record, 164: 545–549.
Ramírez A., R. Ellis, J. Sumar and V. Leyva, 1985. E.coli enteropatógena en alpacas neonatales: aislamiento de intestino delgado y su inoculatión oral. In: Proceedings of the V International Conference on South American Camelids, Cusco, Peru, p. 34.
Twomey D.F., A.M. Barlow, S. Bell, R.M. Chalmers, K. Elwin, M. Giles, R.J. Higgins, G. Robinson and R.M. Stringer, 2008. Cryptosporidiosis in two alpaca (Lama pacos) holdings in the South-West of England. Veterinary Journal, 175: 419–422.
Wales A.D., M.J. Woodward and G.R. Pearson, 2005. Attaching-effacing bacteria in animals. Journal of Comparative Pathology, 132: 1–26.
Whitehead, C.E. and D.E. Anderson, 2006. Neonatal diarrhea in llamas and alpacas. Small Ruminant Research, 61: 207–215.
Wieler L.H., A. Schwanitz, E. Vieler, B. Busse, H. Steinrück, J.B. Kaper and G. Baljer, 1998. Virulence properties of Shiga toxin-producing Escherichia coli (STEC) strains of serogroup O118, a major group of STEC pathogens in calves. Journal of Clinical Microbioly, 36: 1604–1607.
Acknowledgements
This work has been supported by the Universidad Complutense de Madrid, Proyecto de Cooperación International para el Desarrollo V convocatoria, and by the Agencia Española de Cooperación International para el Desarrollo (AECID), Proyecto A/017921/08.
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Cid, D., Martín-Espada, C., Maturrano, L., García, A., Luna, L., Rosadio, R. (2011). Diarrheagenic Escherichia coli strains isolated from neonatal Peruvian alpacas (Vicugna pacos)with diarrhea. In: Pérez-Cabal, M.Á., Gutiérrez, J.P., Cervantes, I., Alcalde, M.J. (eds) Fibre production in South American camelids and other fibre animals. Wageningen Academic Publishers, Wageningen. https://doi.org/10.3920/978-90-8686-727-1_29
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DOI: https://doi.org/10.3920/978-90-8686-727-1_29
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