Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field

Hugo Canahua-Loza; Paul Huanca-Zuñiga; Belinda Chavez; Leslie Canahua-Sosa; Jonathan Almirón; Danny Tupayachy-Quispe

doi:10.4028/p-0z37mj

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HomeMaterials Science ForumMaterials Science Forum Vol. 1060Biological Characterization of Different Samples...

Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field

Abstract:

In this study, the degree of acetylation (DA) and degree of deacetylation (DDA) were determined by Fourier Transform Infrared Spectroscopy (FT-IR) analysis, and their viability was evaluated by the cytocompatibility test with L132 epithelial cells, from samples of chitin and chitosan extracted of Cryphiops caementarius. FT-IR measurements resulted in the successful determination of DA and DDA. Particularly the results showed that, the chitosan CHT-C has the highest DDA (93%), while viability tests employed to determine the cytotoxicity of chitin and chitosan showed that the cell survival rate of CH-C and CHT-C are 130% and 75% respectively while commercial CHT has a rate of 80% approximately, demonstrating that the CH-C (chitin) a favorable behavior against L132 cell.