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CC BY-NC-ND 4.0 · European Journal of General Dentistry 2020; 9(03): 157-162
DOI: 10.4103/ejgd.ejgd_134_20
Original Article

Effects of the Incorporation of Alpha-Tocopherol as Antioxidant on Biological and Physicochemical Properties of Calcium Hydroxide Associated with Bioactive Vehicle

Vitor De Souza
Department of Restorative Dentistry, School of Dentistry Araraquara, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil
,
Joissi Ferrari Zaniboni
Department of Restorative Dentistry, School of Dentistry Araraquara, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil
,
Wilfredo Gustavo Escalante-Otárola
Department of Restorative Dentistry, School of Dentistry Araraquara, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil
,
Mariza Akemi Matsumoto
1   Department of Basic Sciences, Araçatuba Dental School, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil
,
Claudia Cristina Bighetti
1   Department of Basic Sciences, Araçatuba Dental School, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil
,
Milton Carlos Kuga
Department of Restorative Dentistry, School of Dentistry Araraquara, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil
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Abstract

Aim: The aim of this study to evaluate the effects of 10% alpha-tocopherol (10AT) or 20% alpha-tocopherol (20AT) incorporation on biological compatibility, pH, and calcium release of calcium hydroxide (CH) paste associated with 2% chlorhexidine digluconate (CHX). Materials and Methods: Four groups were evaluated: CH, CH-CHX, CH-CHX-10AT, and CH-CHX-20AT. For biological compatibility test, polyethylene tubes containing several pastes were implanted in Wistar rats' subcutaneous tissue (n = 28). After 48 h and 7, 14, and 21 days postimplantation, the specimens were removed and subjected to histologic and histomorphometric analysis. The number of inflammatory cells was evaluated. For pH and calcium release analysis, the pastes were placed individually (n = 10) in plastic tubes and immersed in deionized water. The calcium release and pH changes were evaluated in 24 and 48 h and 7, 14, and 21 days. All data were submitted to Kruskal–Wallis test (α = 0.05). Results: Concerning biological compatibility, all materials shown a similar decidual response (P > 0.05). In the first hours, there was as increase in the number of inflammatory cells, inducing an expressive inflammatory response. After 14 days, inflammation reaction decreased and collagen fiber was organized for the tested pastes (P = 0.05). The pH analysis of the groups maintained the same relationship during the different periods evaluated: the CH and CH-CHX groups showed higher values and were similar to each other (P > 0.05), followed by the CH-CHX-10AT and CH-CHX-20 AT groups. Regarding the amount of calcium ions, in the initial (24 hours) and final (21 days) periods, the groups did not present differences between them (P > 0.05). Conclusion: The 10AT or 20AT, as an antioxidant agent, incorporation to CH and 2% CHX paste negatively affected biological and physicochemical properties.

Keywords

Antioxidants - calcium hydroxide - calcium release - chlorhexidine - subcutaneous tissue

Financial support and sponsorship

Nil.




Publication History

Article published online:
01 November 2021

© 2020. European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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