Anther culture in Physalis peruviana L. Microspore stages, sterilization methods and culture media

Authors

  • Gerardo Mállap-Detquizán Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, Perú. https://orcid.org/0000-0002-0001-9620
  • Jegnes B. Meléndez-Mori Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, Perú. https://orcid.org/0000-0002-1489-7395
  • Eyner Huaman-Huaman Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, Perú. https://orcid.org/0000-0002-3772-5334
  • Nuri C. Vilca-Valqui Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, Perú. https://orcid.org/0000-0002-4539-1602
  • Manuel Oliva Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza (UNTRM), Chachapoyas 01001, Perú. https://orcid.org/0000-0002-9670-0970

DOI:

https://doi.org/10.51372/bioagro351.4

Keywords:

Embryogenesis, flower buds, media components, microspores

Abstract

Anther culture is a tool that allows the production of homozygous double haploid plants, and is a proper and viable alternative for crop improvement. However, the success of its application depends on a number of factors that affect its efficiency. Thus, the objective of this study was to determine the microspore stages associated with flower bud development, as well as to evaluate sterilization methods and development of Physalis peruviana anthers in four culture media. The late uninucleate stage, suitable for initiating androgenesis, was found in flower buds between 7.85 and 9.37 mm polar length. The lowest levels of oxidation (10 %) and contamination (20 %), and the highest anther viability (70 %) were recorded under surface sterilization treatment with ethanol (70 %) for 3 min and NaClO (2 %) for 15 min. Callus formation and green plant regeneration were obtained on B5 and LS media, indicating their usefulness in breeding programs.

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Published

2022-12-31

How to Cite

Mállap-Detquizán, G., Meléndez-Mori, J. B., Huaman-Huaman, E., Vilca-Valqui, N. C., & Oliva, M. (2022). Anther culture in Physalis peruviana L. Microspore stages, sterilization methods and culture media. Bioagro, 35(1), 33-42. https://doi.org/10.51372/bioagro351.4

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