Journal of the American Pharmaceutical Association (Scientific ed.)
ArticlesThe analysis of solutions of epinephrine and norepinephrine*
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Modern Laboratory Evaluation of Peptide and Amines: A Continuing Role for Radioimmunoassay?
2011, Endocrinology and Metabolism Clinics of North AmericaCitation Excerpt :Although Markey and colleagues51 found these methods to be effective for tissues, they were not readily applicable to aqueous biologic fluids (CSF, blood, urine). Markey and colleagues were, however, able to use the method developed by Welsh52 to quantitatively separate biogenic amines from aqueous solutions, a method that involved acetylation by acetic anhydride, or by propionic or deuteroacetic anhydride in cases where endogenous N-acetylated serotonin, such as the pineal gland, might be found. This step was followed by extraction into a nonpolar solvent and a second derivatization step with pentafluoropropionic anhydride was performed.51
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Department of Health, Education and Welfare, Food and Drug Administration, Division of Pharmaceutical Chemistry, Washington, D.C.