The analysis of solutions of epinephrine and norepinephrine

doi:10.1002/jps.3030440815

Journal of the American Pharmaceutical Association (Scientific ed.)

Volume 44, Issue 8, August 1955, Pages 507-514

https://doi.org/10.1002/jps.3030440815 Get rights and content

Abstract

A method of analysis is described which is applicable to solutions of epinephrine and norepinephrine, such as epinephrine injection U. S. P. and levarterenol bitartrate injection N. N. R. The method depends on the quantitative conversion of the substances into O³, O⁴, N‐triacetyl derivatives which may be extracted into chloroform, weighed, identified, and examined polarimetrically for the determination of stereo‐chemical composition. Excellent recoveries of the physiologically potent l‐isomer are obtained, and results of analyses of solutions of epinephrine show very good agreement with potencies determined by the U. S. P. XIV dog bioassay. By supplementing the method with partition chromatography of the acetylation product, the norepinephrine in a solution of U. S. P. epinephrine may be quantitatively isolated as its triacetyl derivative and determined colorimetrically after deacetylation and oxidation to noradrenochrome.

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^*: Department of Health, Education and Welfare, Food and Drug Administration, Division of Pharmaceutical Chemistry, Washington, D.C.

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ArticlesThe analysis of solutions of epinephrine and norepinephrine*

Abstract

United States Pharmacopeia

Anal. Chem.

Quart. J. Pharm. and Pharmacol.

Biochem. J.

Acta pharmacol.

26

Pharmacology in Medicine

Dansk Tids. Farm.

Chem. Zentr.

Chem. Zentr.

Quart. J. Pharm. and Pharmacol.

Quart. J. Pharm. and Pharmacol.

THIS JOURNAL

J. Pharm. and Pharmacol.

J. Am. Chem. Soc.

Articles
The analysis of solutions of epinephrine and norepinephrine*