Elsevier

Experimental Cell Research

Volume 252, Issue 2, 1 November 1999, Pages 273-280
Experimental Cell Research

Regular Article
Isolation and Characterization of a Near-Haploid Human Cell Line

https://doi.org/10.1006/excr.1999.4656Get rights and content

Abstract

Mammalian somatic cells are usually diploid. Occasional rare human tumors have been shown to have a hypodiploid karyotype. We have isolated a near-haploid subclone (P1-55) from a heterogeneous human leukemia cell line, KBM-7. These near-haploid cells have approximately half the human diploid DNA content and have a haploid karyotype except for a disomy of chromosome 8 (25, XY, +8, Ph+). This cell line maintains a majority of cells with a near-haploid karyotype for at least 12 weeks in culture. By serial subcloning, we have isolated near-haploid subclones that maintain ploidy for at least 8 months in culture. Near-haploid cells can also be efficiently isolated from mixed ploidy cultures by size selection. The availability of this human near-haploid cell line should facilitate the genetic analysis of cultured human cells.

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    1

    Permanent address: Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.

    2

    These authors contributed equally to the work presented in this report.

    3

    Current address: Axys Pharmaceuticals, 11099 North Torrey Pines Road, La Jolla, CA 92037.

    4

    To whom correspondence and reprint requests should be addressed. Fax: 617-636-6745. E-mail: [email protected].

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