Elsevier

Experimental Cell Research

Volume 272, Issue 2, 15 January 2002, Pages 109-118
Experimental Cell Research

Regular Article
MT1-MMP-Dependent and -Independent Regulation of Gelatinase A Activation in Long-Term, Ascorbate-Treated Fibroblast Cultures: Regulation by Fibrillar Collagen

https://doi.org/10.1006/excr.2001.5403Get rights and content

Abstract

Human skin fibroblasts were cultured long-term in the presence of ascorbic acid to allow formation of a three-dimensional collagen matrix, and the effects of this on activation of secreted matrix metalloproteinase-2 (MMP-2) were examined. Accumulation of collagen over time correlated with increased levels of both mature MMP-2 and cell-associated membrane type 1-MMP (MT1-MMP), and subsequently increased mRNA levels for MT1-MMP, providing temporal resolution of the “nontranscriptional” and “transcriptional” effects of collagen on MT-1MMP functionality. MMP-2 activation by these cultures was blocked by inhibitors of prolyl-4-hydroxylase, or when fibroblasts derived from the collagen α1(I) gene-deficient Mov-13 mouse were used. MMP-2 activation by the Mov-13 fibroblasts was rescued by transfection of a full-length α1(I) collagen cDNA, and to our surprise, also by transfection with an α1(I) collagen cDNA carrying a mutation at the C-proteinase cleavage, which almost abrogated fibrillogenesis. Although studies with ascorbate-cultured MT1-MMP−/− fibroblasts showed that MT1-MMP played a significant role in the collagen-induced MMP-2 activation, a residual MT1-MMP-independent activation of MMP-2 was seen which resembled the level of MMP-2 activation persisting when wild-type fibroblasts were cultured in the presence of both ascorbic acid and MMP inhibitors. We were also unable to block this residual activation with inhibitors specific for serinyl, aspartyl, or cysteinyl enzymes.

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  • Cited by (0)

    1

    Current address: Department of Biochemistry, University of Hong Kong, Hong Kong.

    2

    To whom correspondence and reprint requests should be addressed at VBCRC Invasion & Metastasis Unit, St. Vincent's Institute of Medical Research, 9 Princes St., Fitzroy, 3065, Australia. Fax: +61-3-9416-2676. E-mail: [email protected].

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