Elsevier

Genomics

Volume 70, Issue 1, 15 November 2000, Pages 74-81
Genomics

Regular Article
Cloning, Mapping, and Expression Analysis of a Gene Encoding a Novel Mammalian EGF-Related Protein (SCUBE1)☆

https://doi.org/10.1006/geno.2000.6370Get rights and content

Abstract

The epidermal growth factor (EGF) superfamily comprises a diverse group of proteins that function as secreted signaling molecules, growth factors, and components of the extracellular matrix, many with a role in vertebrate development. We have isolated a novel mammalian gene encoding an EGF-related protein with a CUB (C1s-like) domain that defines a new mammalian gene family. The Scube1 (signal peptide-CUB domain-EGF-related 1) gene was isolated from a developing mouse urogenital ridge cDNA library and is expressed prominently in the developing gonad, nervous system, somites, surface ectoderm, and limb buds. We have mapped Scube1 to mouse chromosome 15 and show that it is orthologous to a human gene in the syntenic region of chromosome 22q13. We discuss the possible functions of this novel gene and its role in heritable disease in light of these data.

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    ☆

    Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under Accession No. AF276425.

    1

    Present address: Queensland Institute of Medical Research, P.O. Box Royal Brisbane Hospital, Herston, QLD 4029, Australia.

    2

    To whom correspondence should be addressed. Telephone: +44 (01235) 834393. Fax: +44 (01235) 834776. E-mail: [email protected].

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