Regular ArticleStrain differentiation of isolates of streptococci from bovine mastitis by pulsed-field gel electrophoresis
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Cited by (48)
Streptococcus dysgalactiae ssp. dysgalactiae in Norwegian bovine dairy herds: Risk factors, sources, and genomic diversity
2022, Journal of Dairy ScienceCitation Excerpt :The genetic diversity of SDSD from Norwegian dairy herds is unknown, and we do not know whether certain bacterial genotypes are more often associated with mastitis than others. Previous studies investigating between-herd diversity of SDSD have used gel-based genotyping methods (Baseggio et al., 1997; Wang et al., 1999; Lundberg et al., 2014; Wente and Krömker, 2020) or biotyping (Aarestrup and Jensen, 1996). New studies using whole-genome sequencing (WGS), which has higher resolution, to investigate within-herd and between-herd diversity of SDSD are warranted.
Molecular epidemiology of Streptococcus uberis intramammary infections: Persistent and transient patterns of infection in a dairy herd
2020, Journal of Dairy ScienceCitation Excerpt :Direct transmission from an infected quarter to uninfected quarters would likely occur during the milking process. Occurrence of a limited number of strains in a herd is generally associated with a contagious pathogen such as Streptococcus agalactiae and Staphylococcus aureus (Lam et al., 1996; Baseggio et al., 1997; Wang et al., 1999). Our results suggest that contagious transmission may also occur in the case of S. uberis IMI.
Associations between Streptococcus uberis strains from the animal environment and clinical bovine mastitis cases
2019, Journal of Dairy ScienceGenotyping of Streptococcus uberis isolates in healing process of bovine clinical mastitis
2018, International Journal of Veterinary Science and MedicineCitation Excerpt :The preparation of whole bacterial DNA was carried out in agarose gel plugs followed by the digestion of the bacterial genome with the restriction enzyme SmaI. This step was then followed by fragment separation by PFGE using the pulse time described by Baseggio et al. [17]. The interpretation of the restriction patterns was performed as described by Tenover et al. [18].
Genetic diversity, virulence genes, and antimicrobial resistance of Streptococcus dysgalactiae isolates from different aquatic animal sources
2017, AquacultureCitation Excerpt :In contrast, bacterial strains harboring no tetracycline or macrolide resistance genes were susceptible to the corresponding drugs (Table 6). PFGE was considered as a “gold standard” molecular typing method with high epidemiological concordance and excellent discriminatory power for streptococci clinical studies (Baseggio et al., 1997; Wang et al., 1999; Sabat et al., 2013) and also employed for S. dysgalactiae isolates in fish (Abdelsalam et al., 2010a, 2010b, 2015; Tsai et al., 2015; Costa et al., 2014). Abdelsalam et al. (2010a, 2010b, 2015) found that 30 strains isolated from distinct geographical sources between 2003 and 2007 exhibited different genomic profiles, with ApaI digestion providing greater discriminatory power during PFGE than SmaI.
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