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Experimental designs for rumen microbiology

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Book cover Methods in Gut Microbial Ecology for Ruminants

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References

  1. Abe, M., T. Iriki, N. Tobe, and H. Shibu. 1981. Sequestration of holotrich protozoa in the reticulo-rumen of cattle. Appl. Environ. Microbiol. 41:758–765.

    CAS  PubMed  Google Scholar 

  2. Akin, D.E. 1976. Ultrastructure of rumen bacterial attachment to forage cell walls. Appl. Environ. Microbiol. 31:562–568.

    CAS  PubMed  Google Scholar 

  3. Amman, R.I., W. Ludwig, and K.H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143–169.

    Google Scholar 

  4. Anderson, K.L. 2003. The complex world of gastrointestinal bacteria. Can. J. Anim. Sci. 83:409–427.

    CAS  Google Scholar 

  5. Anderson, K.L., T.G. Nagaraja, J.L. Morrill, T.B. Avery, S.J. Galitzer, and J.E. Boyer. 1987. Ruminal microbial development in conventionally or early-weaned calves. J. Anim. Sci. 64:1215–1226.

    CAS  PubMed  Google Scholar 

  6. Baldwin, R.L. 1995. Modelling Ruminant Digestion and Metabolism. pp. 469–518. Chapman and Hall, NY.

    Google Scholar 

  7. Bauchop, T. 1989. Colonization of plant fragments by protozoa and fungi, pp. 83–96. In J.V. Nolan, R.A. Leng, and D.I. Demeyer (eds.), The Roles of Protozoa and Fungi in Rumen Digestion. Penambul Books, Armidale, NSW.

    Google Scholar 

  8. Bryant, M.P., and L.A. Burkey. 1953. Numbers and some predominant groups of bacteria in the rumen of cows fed different rations. J. Dairy Sci. 36:218.

    Google Scholar 

  9. Bryant, M.P., and I.M. Robinson. 1968. Effects of diet, time after feeding and position of sampling on the numbers of viable bacteria in the bovine rumen. J. Dairy Sci. 51:1950–1955.

    CAS  PubMed  Google Scholar 

  10. Bryant, M.P., N. Small, C. Bouma, and I. Robinson. 1958. Studies on the composition of the rumen flora and fauna of young calves. J. Dairy Sci. 41:1747–1767.

    Google Scholar 

  11. Chen, M., and M.J. Wolin. 1979. Effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen saccarolytic bacteria. Appl. Environ. Microbiol. 38:72–78.

    CAS  PubMed  Google Scholar 

  12. Clarke, R.T.J., M.J. Ulyatt, and A. John. 1982. Variation in numbers and mass of ciliate protozoa in the rumens of sheep fed chaffed alfalfa (Medicago sativa). Appl. Environ. Microbiol. 43:1202–1204.

    Google Scholar 

  13. Craig, W.M., G.A. Broderick, and D.B. Ricker. 1987. Quantitation of microorganisms associated with the particulate phase of ruminal digesta. J. Nutr. 117:56–64.

    CAS  PubMed  Google Scholar 

  14. Davies, D.R., M.K. Theodorou, M.I.G. Lawrence, and A.P.J. Trinci. 1993. Distribution of anaerobic fungi in the digestive tract of cattle and their survival in faeces. J. Gen. Microbiol. 139:1395–1400.

    PubMed  Google Scholar 

  15. Dehority, B.B., and C.G. Orpin. 1997. Development of and natural fluctuations in rumen microbial populations, pp. 196–245. In P.N. Hobson and C.S. Stewart (eds.), The Rumen Microbial Ecosystem. Chapman and Hall, London.

    Google Scholar 

  16. Dijkstra, J. 1994. Simulation of the dynamics of protozoa in the rumen. Br. J. Nutr. 72:679–699.

    CAS  PubMed  Google Scholar 

  17. Dixon, R.M., W. Karda, B.J. Hosking, and A.R. Egan. 2001. Oilseed meals and fortified grain as supplements fed infrequently to sheep consuming low quality roughage. Anim. Feed Sci. Technol. 50:248–257.

    Google Scholar 

  18. Eadie, J.M. 1962. The development of rumen microbial populations in lambs and calves under various conditions of management. J. Gen. Microbiol. 29:563–578.

    Google Scholar 

  19. Egan, A.R., and M.J. Ulyatt. 1980. Quantitative digestion of fresh herbages by sheep: VI. Utilization of N of five herbages. J. Agric. Sci. Camb. 94:47–56.

    CAS  Google Scholar 

  20. Faichney, G.J. 1993. Digesta flow, pp. 53–86. In J.M Forbes and J. France (eds.), Quantitative Aspects of Ruminant Digestion and Metabolism. CAB International, Wallingford, UK.

    Google Scholar 

  21. Fonty, G., J.P. Jounay, J. Senaud, P. Gouet, and J. Grain. 1984. The evolution of microflora, microfauna and digestion in the rumen of lambs from birth to 4 months. Can. J. Anim. Sci. 64(Suppl.):165–166.

    Google Scholar 

  22. Fox, D.G., L.O. Tedeschi, T.P. Tylutki, J.B. Russell, M.E. van Amburgh, L.E. Chase, A.N. Pell, and T.R. Overton. 2004. The Cornell Net Carbohydrate and Protein System model for evaluating herd nutrition and nutrient excretion. Anim. Feed Sci. Technol. 112:29–78.

    Article  CAS  Google Scholar 

  23. Gordon, G.L.R., and M.W. Phillips. 1993. Removal of anaerobic fungi from the rumen of sheep by chemical treatment and the effect on feed consumption and in vivo fibre digestion. Lett. Appl. Microbiol. 17:220–223.

    Google Scholar 

  24. Hespell, R.B., D.E. Akin, and B.A. Dehority. 1996. Bacteria, fungi and protozoa of the rumen, Vol. II, pp. 59–141. In R.I. Mackie, B.A. White, and R.E. Isaacson (eds.), Gastrointestinal Microbiology. Chapman and Hall, NY.

    Google Scholar 

  25. Hespell, R.B., and M.P. Bryant. 1979. Efficiency of microbial growth: influence of some theoretical and experimental factors on YATP. J. Anim. Sci. 49:1640–1659.

    CAS  PubMed  Google Scholar 

  26. Jarvis, G.N., C. Strompl, D.M. Burgess, L.C. Skillman, E.R. Moore, and K.N. Joblin. 2000. Isolation and identification of ruminal methanogens from grazing cattle. Curr. Microbiol. 40:327–332.

    Article  CAS  PubMed  Google Scholar 

  27. Jounay, J.P., B. Michalet-Doreau, and M. Doreau. 2000. Manipulation of the rumen ecosystem to support high-performance beef cattle — review. Asian Australas. J. Anim. Sci. 13:96–114.

    Google Scholar 

  28. Kocherginskaya, S.A., R.L. Aminov, and B.A. White. 2001. Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing and statistical ecological approaches. Anaerobe 7:119–134.

    Article  CAS  Google Scholar 

  29. Krause, D.O., W.J.M. Smith, F.M.E. Ryan, R.I. Mackie, and C.S. McSweeney. 2000. Use of 16S-rDNA based techniques to investigate the ecological succession of microbial populations in the immature lamb rumen: Tracking of a specific strain of inoculated Ruminococcus and interactions with other microbial populations in vivo. Microbiol. Ecol. 38:365–376.

    Google Scholar 

  30. Krysl, L.J., M.E. Branine, A.U. Cheema, A.U. Funk, and A.L. Galyean. 1989. Influence of soybean meal and sorghum grin supplements on intake, digesta kinetics, ruminal fermentation, site and extent of digestion and microbial protein synthesis in beef steers grazing blue grama rangeland. J. Anim. Sci. 61:3040–3051.

    Google Scholar 

  31. Larue, R., Z. Yu, V.A. Parisi, A.R. Egan, and M. Morrison. 2005. Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing. Environ. Microbiol. 7:530–543.

    Article  CAS  PubMed  Google Scholar 

  32. Mackie, R.I., F.M.C. Gilchrist, A.M. Roberts, P.E. Hannah, and H.M. Schwartz. 1978. Microbiological and chemical changes in the rumen during stepwise adaptation of sheep to high concentrate diets. J. Agric. Sci. Camb. 90:241–254.

    CAS  Google Scholar 

  33. Mackie, R.I., A. Sghir, and H.R. Gaskins. 1999. Developmental microbial ecology of the neonatal gastrointestinal tract. Am. J. Clin. Nutr. 69:1035S–1045S.

    CAS  PubMed  Google Scholar 

  34. Mackie, R.I., and B.A. White. 1990. Recent advances in rumen microbial ecology and metabolism: potential impact on nutrient output. J. Dairy Sci. 73:2971–2995.

    CAS  PubMed  Google Scholar 

  35. Martin, C., and B. Michalet-Doreau. 1995. Variations in mass and enzyme activity of rumen microorganisms: effect of barley and buffer supplements. J. Sci. Food Agric. 67:407–413.

    CAS  Google Scholar 

  36. Rasmussen, M.A., B.A. White, and R.B. Hespell. 1989. Improved assay fro quantitating adherence of ruminal bacteria to cellulose. Appl. Environ. Microbiol. 55:2089–2091.

    CAS  PubMed  Google Scholar 

  37. Romulo, B., S.H. Bird, and R.A. Leng. 1989. Effects of defaunation and protein supplementation on intake, digestibility, Nretention and fungal numbers in sheep fed straw-based diets, pp. 285–288. In J.V. Nolan, R.A. Leng, and D.I. Demeyer (eds.), The Role of Prototzoa and Fungi in Ruminant Digestion. Penambul Books, Armidale, NSW, Australia.

    Google Scholar 

  38. Russell, J.B., W.M. Sharp, and R.L. Baldwin. 1979. The effect of pH on maximum bacterial growth rate and its possible role as a determinant of bacterial competition in the rumen. J. Anim. Sci. 48:251–255.

    CAS  PubMed  Google Scholar 

  39. Russell, J.B., and H.J. Strobel. 1993. Microbial energetics, pp. 165–186. In J.M. Forbes and J. France (eds.), Quantitative Aspects of Ruminant Digestion and Metabolism. CAB International, Wallingford, UK.

    Google Scholar 

  40. Stewart, C.S., H.J. Flint, and M.P. Bryant. 1997. The rumen bacteria, pp. 10–72. In P.N. Hobson and C.S. Stewart (eds.), The Rumen Microbial Ecosystem. Chapman and Hall, London.

    Google Scholar 

  41. Tajima, K., R.I. Aminov, T. Nagamine, K. Ogata, M. Nakamura, H. Matsui, and Y. Benno. 1999. Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. FEMS Microbiol. Ecol. 29:159–169.

    CAS  Google Scholar 

  42. Walker, D.J., A.R. Egan, C.J. Nader, M.J. Ulyatt, and G.B. Storer. 1975. Rumen microbial protein synthesis and the proportions of microbial and non-microbial N flowing to the intestines of sheep. Aust. J. Agric. Res. 26:699–708.

    CAS  Google Scholar 

  43. Warner, A.C.I. 1966. Diurnal changes in the concentrations of microorganisms in the rumens of sheep fed to appetite in pens or pasture. J. Gen. Microbiol. 45:243–251.

    CAS  PubMed  Google Scholar 

  44. Williams, A.G., K.N. Joblin, and G. Fonty. 1994. Interactions between the rumen chytrid fungi and other microorganisms, pp.191–228. In D.O. Mountford and C.G. Orpin (eds.), Anaerobic Fungi. Marcel Dekker, NY.

    Google Scholar 

  45. Williams, Y.J., G.P. Walker, P.T. Doyle, A.R. Egan, and C.R. Stockdale. 2005. Rumen fermentation characteristics of dairy cows grazing different allowances of Persian clover-or perennial ryegrassdominant swards in spring. Aust. J. Exp. Agric. 45:665–675.

    Google Scholar 

  46. Wolin, M.J. 1979. The rumen fermentation: a model for microbial interactions in anaerobic systems. Adv. Micro. Ecol. 3:49–77.

    CAS  Google Scholar 

  47. Wolin, M.J., T.L. Miller, and C.S. Stewart. 1997. Microbe-microbe interactions, p. 467–491. In P.N. Hobson and C.S. Stewart (eds.), The Rumen Microbial Ecosystem. Chapman and Hall, London.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Land and Food Resources, The University of Melbourne, Parkville, Victoria, 3010, Australia

    Adrian R. Egan

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  1. Adrian R. Egan
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Editors and Affiliations

  1. Animal Production and Health Section Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, Austria

    Harinder P.S. Makkar

  2. CSIRO Livestock Industries, Queensland Bioscience Precinct, St Lucia, Queensland, Australia

    Christopher S. McSweeney

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Egan, A.R. (2005). Experimental designs for rumen microbiology. In: Makkar, H.P., McSweeney, C.S. (eds) Methods in Gut Microbial Ecology for Ruminants. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3791-0_1

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