Abstract
Hematopoietic stem cells (HSC) act as paradigmatic tissue-specific adult stem cells. While they are quiescent in steady-state conditions, they enter the cell cycle and proliferate in stress conditions and during tissue regeneration. Therefore, analysis of cell cycle status of HSC is crucial for understanding their biology. However, due to low number of HSC in tissue and need to use many surface markers for their identification, analysis of their cycle status is technically complicated. Here, we presented our simple strategy to analyze cell cycle of strictly defined LKS CD48−CD150+CD34− HSC, together with Ki67 and DAPI staining by flow cytometry.
*Author contributed equally with all other contributors.
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Acknowledgments
The research was supported by grants: HARMONIA (NCN2015/18/M/NZ3/00387) granted to Alicja Jozkowicz by Polish National Science Centre and PRELUDIUM (NCN2013/11/N/NZ3/00956) granted to Krzysztof Szade by Polish National Science Centre. The Faculty of Biochemistry, Biophysics, and Biotechnology of Jagiellonian University is a partner of the Leading National Research Centre (KNOW) supported by the Ministry of Science and Higher Education.
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Szade, K., Bukowska-Strakova, K., Zukowska, M., Jozkowicz, A., Dulak, J. (2016). Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells. In: Turksen, K. (eds) Stem Cell Heterogeneity. Methods in Molecular Biology, vol 1516. Humana Press, New York, NY. https://doi.org/10.1007/7651_2016_361
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DOI: https://doi.org/10.1007/7651_2016_361
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6549-6
Online ISBN: 978-1-4939-6550-2
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