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Biofilm Formation and Quantification Using the 96-Microtiter Plate

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Leptospira spp.

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2134))

Abstract

Biofilm formation in microtiter plates is certainly the most commonly used method to grow and study biofilm. This simple design is very popular due to its high-throughput screening capacities, low cost, and easy handling. In the protocol described here, we focus on the use of 96-well optically clear, polystyrene flat-bottom plate to study biofilm formation by Leptospira spp. and quantify the biofilm formation by crystal violet (CV) staining. We also describe an alternative method, based on phase contrast image analysis that we believe is more suitable for accurately quantifying biofilm growth by reducing handling of this fragile structure.

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Acknowledgments

This work was supported by an AXA Research Funds grant “AXA Postdoctoral Fellowship,” 15-AXA-PDOC-037 granted to RT.

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Authors and Affiliations

  1. Leptospirosis Research and Expertise Unit, Institut Pasteur in New Caledonia, Institut Pasteur International Network, Noumea, New Caledonia, France

    Roman Thibeaux, Malia Kainiu & Cyrille Goarant

Authors
  1. Roman Thibeaux
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  2. Malia Kainiu
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  3. Cyrille Goarant
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Corresponding author

Correspondence to Roman Thibeaux .

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Editors and Affiliations

  1. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan

    Nobuo Koizumi

  2. Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France

    Mathieu Picardeau

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Thibeaux, R., Kainiu, M., Goarant, C. (2020). Biofilm Formation and Quantification Using the 96-Microtiter Plate. In: Koizumi, N., Picardeau, M. (eds) Leptospira spp.. Methods in Molecular Biology, vol 2134. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0459-5_19

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  • DOI: https://doi.org/10.1007/978-1-0716-0459-5_19

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0458-8

  • Online ISBN: 978-1-0716-0459-5

  • eBook Packages: Springer Protocols

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