Abstract
To date, only three bacteriophages of leptospires—leptophages—are known. Nonetheless, numerous prophages have been found in the genus, especially in the genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization.
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Acknowledgments
This work was part of the Ph.D. thesis of O.S. who received financial support from “Université Paris Diderot” and “Sorbonne Paris Cité.” We thank Soizick Lucas-Staat and Nicolas Dufour for technical assistance during leptophage extractions, Olivier Gorgette for technical assistance during the TEM observations, and Robert A. Gaultney for proofreading the manuscript.
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Schiettekatte, O., Bourhy, P. (2020). Isolation, Purification, and Characterization of Leptophages. In: Koizumi, N., Picardeau, M. (eds) Leptospira spp.. Methods in Molecular Biology, vol 2134. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0459-5_7
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DOI: https://doi.org/10.1007/978-1-0716-0459-5_7
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