Abstract
The preventive and therapeutic potential of DNA vaccines combined with benefits of lipid-based delivery (lipofection) allow efficient nucleic acid transfer and immunization applicable in treatment of infections, cancer or autoimmune disorders. Lipofecting compositions consisting of cationic and neutral lipids can be used for both in vitro and in vivo applications and may also play the role of adjuvants. Here we describe a simple protocol of DNA vaccine carrier preparation based on cationic polyprenyl derivatives (PTAI—trimethylpolyprenylammonium iodides) and commonly used helper lipids with use of basic laboratory equipment. Such formulas have proven effective for immunization of animals as well as for cell transfection.
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References
Comberlato A, Paloja K, Bastings MMC (2019) Nucleic acids presenting polymer nanomaterials as vaccine adjuvants. J Mater Chem B 7:6321. https://doi.org/10.1039/c9tb01222b
Myhr AI (2017) DNA vaccines: regulatory considerations and safety aspects. Curr Issues Mol Biol 22:79–88. https://doi.org/10.21775/cimb.022.079
Hobernik D, Bros M (2018) DNA vaccines—how far from clinical use? Int J Mol Sci 19. https://doi.org/10.3390/ijms19113605
Un K, Kawakami S, Suzuki R et al (2010) Development of an ultrasound-responsive and mannose-modified gene carrier for DNA vaccine therapy. Biomaterials 31:7813–7826. https://doi.org/10.1016/j.biomaterials.2010.06.058
Christensen D, Korsholm KS, Rosenkrands I et al (2007) Cationic liposomes as vaccine adjuvants. Expert Rev Vaccines 6:785–796. https://doi.org/10.1586/14760584.6.5.785
Firouzmand H, Badiee A, Khamesipour A et al (2013) Induction of protection against leishmaniasis in susceptible BALB/c mice using simple DOTAP cationic nanoliposomes containing soluble Leishmania antigen (SLA). Acta Trop 128:528–535. https://doi.org/10.1016/j.actatropica.2013.07.021
Carroll TD, Matzinger SR, Barry PA et al (2014) Efficacy of influenza vaccination of elderly rhesus macaques is dramatically improved by addition of a cationic lipid/DNA adjuvant. J Infect Dis 209:24–33. https://doi.org/10.1093/infdis/jit540
Korsholm KS, Andersen PL, Christensen D (2012) Cationic liposomal vaccine adjuvants in animal challenge models: overview and current clinical status. Expert Rev Vaccines 11:561–577. https://doi.org/10.1586/erv.12.22
Stachyra A, Rak M, Redkiewicz P et al (2017) Effective usage of cationic derivatives of polyprenols as carriers of DNA vaccines against influenza virus. Virol J 14:168. https://doi.org/10.1186/s12985-017-0838-x
Erasmus JH, Khandhar AP, Guderian J et al (2018) A nanostructured lipid carrier for delivery of a replicating viral RNA provides single, low-dose protection against Zika. Mol Ther 26:2507–2522. https://doi.org/10.1016/j.ymthe.2018.07.010
Wang W, Li W, Ma N, Steinhoff G (2013) Non-viral gene delivery methods. Curr Pharm Biotechnol 14:46–60
Pozzi D, Marchini C, Cardarelli F et al (2014) Mechanistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: interplay between nanostructure and composition. Biochim Biophys Acta 1838:957–967. https://doi.org/10.1016/j.bbamem.2013.11.014
Smisterová J, Wagenaar A, Stuart MC et al (2001) Molecular shape of the cationic lipid controls the structure of cationic lipid/dioleylphosphatidylethanolamine-DNA complexes and the efficiency of gene delivery. J Biol Chem 276:47615–47622. https://doi.org/10.1074/jbc.M106199200
Zuhorn IS, Oberle V, Visser WH et al (2002) Phase behavior of cationic amphiphiles and their mixtures with helper lipid influences lipoplex shape, DNA translocation, and transfection efficiency. Biophys J 83:2096–2108. https://doi.org/10.1016/S0006-3495(02)73970-2
Madeja Z, Rak M, Wybieralska E et al (2007) New cationic polyprenyl derivative proposed as a lipofecting agent. Acta Biochim Pol 54:873–876
Rak M, Ochałek A, Bielecka E et al (2016) Efficient and non-toxic gene delivery by anionic lipoplexes based on polyprenyl ammonium salts and their effects on cell physiology. J Gene Med 18:331–342. https://doi.org/10.1002/jgm.2930
Gawrys O, Polkowska M, Roszkowska-Chojecka M et al (2014) Effects of liposomes with polyisoprenoids, potential drug carriers, on the cardiovascular and excretory system in rats. Pharmacol Rep 66:273–278. https://doi.org/10.1016/j.pharep.2013.09.009
Gawrys O, Olszyński KH, Gawarecka K et al (2014) Cationic derivative of polyprenol, a potential component of liposomal drug carriers, does not alter renal function in rats. Eur J Lipid Sci Technol 116:659–662. https://doi.org/10.1002/ejlt.201300489
Utkina NS, Danilov LL, Sizova OV et al (2010) Synthesis of quaternary ammonium salts containing a polyprenyl substituent. Russ Chem Bull Int Ed 59:1459–1462. https://doi.org/10.1007/s11172-010-0262-5
Acknowledgments
This chapter is dedicated to Professor Tadeusz Chojnacki of the Institute of Biochemistry and Biophysics, Polish Academy of Sciences (PAS) in Warsaw, the Father of Polish Polyprenology who started the cooperation between the Institute of Biochemistry and Biophysics PAS and Jagiellonian University. We would like to thank Professor Ewa Swiezewska of the Institute of Biochemistry and Biophysics PAS for inspiring cooperation, endless supplies of polyprenols, and critical reading of the manuscript. Preparation of cationic derivatives of polyprenols used in a series of projects, which contributed to this chapter, by Professor Marek Chmielewski and Doctor Marek Masnyk of the Institute of Organic Chemistry PAS is greatly appreciated. We also thank all the people from the Polish Vaccine Consortium (PVC) involved in the development of vaccines against influenza virus. This work was, in part, financially supported by the National Science Centre, Poland (2018/02/X/NZ3/01566 to M.R. and 2018/31/B/NZ3/01750 to Z.M.).
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Rak, M., Góra-Sochacka, A., Madeja, Z. (2021). Lipofection-Based Delivery of DNA Vaccines. In: Pfeifer, B.A., Hill, A. (eds) Vaccine Delivery Technology. Methods in Molecular Biology, vol 2183. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0795-4_20
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DOI: https://doi.org/10.1007/978-1-0716-0795-4_20
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