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Periodontal Pathogens pp 113–121Cite as

Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2210))

Abstract

The type IX secretion system (T9SS) is the most recently discovered secretion system in the gram-negative bacteria and is specific to the Bacteroidetes phylum. It is comprised of at least 19 proteins, which together allows for the secretion and cell surface attachment of a specific group of proteins (T9SS substrates), that harbor a signal sequence at the C-terminus. Here we describe the structural characterization of the PorK, PorN and PorG components of the Porphyromonas gingivalis T9SS using electron microscopy and cross-linking mass spectrometry.

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Acknowledgments

This work was supported by the Australian Government Department of Industry, Innovation and Science Grant ID 20080108, the National Health and Medical Research Council Grant ID 1123866.

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Authors and Affiliations

  1. Oral Health Cooperative Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Parkville, VIC, Australia

    Dhana G. Gorasia, Paul D. Veith & Eric C. Reynolds

  2. Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, Australia

    Eric Hanssen

  3. Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, Australia

    Eric Hanssen

Authors
  1. Dhana G. Gorasia
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  2. Eric Hanssen
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  3. Paul D. Veith
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  4. Eric C. Reynolds
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Corresponding authors

Correspondence to Paul D. Veith or Eric C. Reynolds .

Editor information

Editors and Affiliations

  1. Division of Microbiology, Department of Oral Biology School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido, Japan

    Keiji Nagano

  2. Department of Microbiology School of Dentistry, Aichi Gakuin University, Nisshin, Aichi, Japan

    Yoshiaki Hasegawa

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Gorasia, D.G., Hanssen, E., Veith, P.D., Reynolds, E.C. (2021). Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis. In: Nagano, K., Hasegawa, Y. (eds) Periodontal Pathogens. Methods in Molecular Biology, vol 2210. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0939-2_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0939-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0938-5

  • Online ISBN: 978-1-0716-0939-2

  • eBook Packages: Springer Protocols

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