Abstract
Here we describe the application of mass cytometry to analyze tumor-infiltrating lymphocytes in human melanoma. Mass cytometry is the coupling of flow cytometry and mass spectrometry, which allows for the simultaneous measurement of 40+ cell parameters on a per cell basis. Heavy metal-labeled antibodies can bind to proteins (CD markers, transcription factors, cytokines) on the cell surface and in the cytoplasm/nucleus. As labeled cells pass through the CyTOF, the instrument detects the heavy metals. Combining these signals allows description of melanoma tumor-infiltrating lymphocytes at a greater depth than alternative phenotyping strategies and enables detailed analyses of a variety of cellular parameters, including immune cell lineage, activation status, and functional polarization.
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Reference
Sumatoh HR, Teng KWW, Cheng Y, Newell EW (2017) Optimization of mass cytometry sample cryopreservation after staining. Cytometry A 91(1):48–61. https://doi.org/10.1002/cyto.a.23014
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Tantalo, D. et al. (2021). Using Mass Cytometry to Analyze the Tumor-Infiltrating Lymphocytes in Human Melanoma. In: Hargadon, K.M. (eds) Melanoma. Methods in Molecular Biology, vol 2265. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1205-7_38
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DOI: https://doi.org/10.1007/978-1-0716-1205-7_38
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1204-0
Online ISBN: 978-1-0716-1205-7
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