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Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis

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Mass Spectrometry-Based Lipidomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2306))

Abstract

Chemical derivatization coupled with nano-electrospray ionization (nESI) and ultra-high resolution accurate mass spectrometry (UHRAMS) is an established approach to overcome isobaric and isomeric mass interference limitations, and improve the analytical performance, of direct-infusion (i.e., “shotgun”) lipidome analysis strategies for “sum composition” level identification and quantification of individual lipid species from within complex mixtures. Here, we describe a protocol for sequential functional group selective derivatization of aminophospholipids and O-alk-1′-enyl (i.e., plasmalogen) lipids, that when integrated into a shotgun lipidomics workflow involving deuterium-labeled internal lipid standard addition, monophasic lipid extraction, and nESI-UHRAMS analysis, enables the routine identification and quantification of >500 individual lipid species at the “sum composition” level, across four lipid categories and from >30 lipid classes and subclasses.

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Acknowledgments

This work was supported by research grants APP1156778 and APP1142750 from the National Health and Medical Research Council (NHMRC), and research grant DP190102464 from the Australian Research Council (ARC).

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Authors and Affiliations

  1. School of Chemistry, The University of Melbourne, Parkville, VIC, Australia

    Vinzenz Hofferek & Gavin E. Reid

  2. Department of Biochemistry and Pharmacology, The University of Melbourne, Parkville, VIC, Australia

    Huaqi Su & Gavin E. Reid

  3. The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC, Australia

    Huaqi Su

  4. Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia

    Gavin E. Reid

Authors
  1. Vinzenz Hofferek
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  2. Huaqi Su
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  3. Gavin E. Reid
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Editor information

Editors and Affiliations

  1. Mass Spectrometry Resource Division of Endocrinology Metabolism, and Lipid Research Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA

    Fong-Fu Hsu

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Hofferek, V., Su, H., Reid, G.E. (2021). Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis. In: Hsu, FF. (eds) Mass Spectrometry-Based Lipidomics. Methods in Molecular Biology, vol 2306. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1410-5_5

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  • DOI: https://doi.org/10.1007/978-1-0716-1410-5_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1409-9

  • Online ISBN: 978-1-0716-1410-5

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