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Doubled Haploid Production through Microspore Culture

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Accelerated Breeding of Cereal Crops

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Abstract

Isolated microspore culture (IMC) for the production of doubled haploid (DH) plants is a method used by plant breeders to rapidly obtain homozygous breeding lines. Breeders cut many years off the time it takes to create commercial lines by recovering plants from chromosome doubling of haploids. Although there are several DH production methods, IMC has great potential and is highly desirable because large numbers of cells can be isolated and cultured with relative ease. Even with this advantage, however, several factors inhibit universal adoption of microspore culture, especially in wheat. Problematic areas are the rapid death of microspores in culture, the failure of microspore to undergo embryogenesis, and the high percentage of albinos that can be seen in resulting DH plants of certain genotypes. This chapter highlights these challenges and reviews research being done toward finding solutions for IMC improvement with a focus on wheat.

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Acknowledgments

This work was supported by the Alberta Funding Consortium grant (2018F142R) entitled “A Comparative Genomics Approach to Improve Doubled Haploid Breeding for Common and Durum Wheat.” Generous contributors to these funds were the Alberta Agriculture and Forestry, Alberta Wheat Commission, and Saskatchewan Wheat Development Commission.

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  1. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    Theia Jensen, Kyle Bodell, Fengying Jiang & John D. Laurie

  2. The Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada

    Theia Jensen & Kyle Bodell

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  1. Agriculture and Agri-Food Canada, Morden, MB, Canada

    Andriy Bilichak

  2. Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    John D. Laurie

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Jensen, T., Bodell, K., Jiang, F., Laurie, J.D. (2022). Doubled Haploid Production through Microspore Culture. In: Bilichak, A., Laurie, J.D. (eds) Accelerated Breeding of Cereal Crops. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1526-3_6

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  • DOI: https://doi.org/10.1007/978-1-0716-1526-3_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1525-6

  • Online ISBN: 978-1-0716-1526-3

  • eBook Packages: Springer Protocols

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