Abstract
This chapter contains the latest version of essential protocols established to study Salmonella persisters during macrophage infection . These methods, which can be applied to other pathogens, allow researchers to quantify, visualize, and characterize bacterial persisters within a population and within immune cells consistent with the recent consensus statement published by the research community working on antibiotic persistence (Balaban et al, Nat Rev Microbiol 17:441–448, 2019). These protocols notably allow the discrimination between tolerance and persistence during infection , which is essential to clarify which phenomenon is actually reported. Methods described in this chapter may contribute to the determination of key bacterial and host genes that contribute to antibiotic persistence.
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Change history
19 March 2022
An error in the production process unfortunately let to the publication of the book before incorporating the below corrections. This has now been corrected:
References
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Acknowledgments
We thank Julian Rycroft and Rachel Giorgio for their critical reading of the manuscript. S.R. and C.M. were supported by an EMBO long-term fellowship (ALTF 835-2016) to S.R., a Lister Research Prize 2017 and funding from the European Research Council (ERC) (Grant agreement No. 757369) to S.H.
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Michaux, C., Ronneau, S., Helaine, S. (2021). Studying Antibiotic Persistence During Infection. In: Verstraeten, N., Michiels, J. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 2357. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1621-5_18
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DOI: https://doi.org/10.1007/978-1-0716-1621-5_18
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-1621-5
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