Abstract
Membrane trafficking is central to cell plate construction during plant cytokinesis. Studies on cell plate formation can provide answers to basic biology questions including molecular mechanisms of membrane trafficking, tissue patterning, and cytoskeletal dynamics. Consequently, a detailed understanding of cytokinesis depends on the characterization of molecules that function in the formation, transport, targeting, and fusion of membrane vesicles and delivery of proteins to the developing and maturing plate. This chapter describes a pipeline based on fluorescence recovery after photobleaching (FRAP) to measure and analyze turnover of peripheral or transmembrane proteins on the cell plate. The approach described here can also be applied in other biological contexts.
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Acknowledgments
This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme “Human Resources Development, Education and Lifelong Learning 2014–2020” in the context of the project “Membraneless organelles in plants: formation and the role of their interaction with the plasma membrane” (MIS 5048447).
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Deli, A., Tympa, LE., Moschou, P.N. (2022). Analyses of Protein Turnover at the Cell Plate by Fluorescence Recovery After Photobleaching During Cytokinesis. In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 2382. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1744-1_14
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DOI: https://doi.org/10.1007/978-1-0716-1744-1_14
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