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Applications of CPPs in Genome Editing of Plants

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Cell Penetrating Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2383))

Abstract

Cell penetrating peptides (CPPs) are short peptides that are able to translocate themselves and their cargo into cells. The progressive and continuous application of CPPs in various fields of basic and applied research shows that they are efficient delivery vectors for an assortment of biomolecules, including nucleic acids and proteins. This feature makes CPPs an excellent tool for modification of plant genomes through transgenesis and genome editing. In this review, we present the progress during the last three decades in application of CPPs for delivery of DNA, RNA, and proteins into plant cells and tissues. Moreover, we highlight the exploiting of CPPs as advantageous and beneficial tool for plant genome editing via delivery of nuclease proteins, and provide a practical example of genome alternation through CPP-delivered nucleases. Finally, the current exploitation of peptides in organelle-specific DNA delivery and modification of organellar genomes is discussed.

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Acknowledgments

The A.S., J.L., and A.Z. authors thank Agriculture and Agri-Food Canada and Agriculture Funding Consortium (Alberta Innovates/Biosolutions, Alberta Wheat Commission and Saskatchewan Wheat Development Commission) for their financial support. A.B. thanks to Agriculture and Agri-Food Canada for New scientists support fund.

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Authors and Affiliations

  1. Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    Atta Soliman, John Laurie & Alicja Ziemienowicz

  2. Morden Research and Development Centre, Agriculture and Agri-Food Canada, Morden, MB, Canada

    Andriy Bilichak

Authors
  1. Atta Soliman
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  2. John Laurie
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  3. Andriy Bilichak
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  4. Alicja Ziemienowicz
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Correspondence to Alicja Ziemienowicz .

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  1. Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden

    Ãœlo Langel

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Soliman, A., Laurie, J., Bilichak, A., Ziemienowicz, A. (2022). Applications of CPPs in Genome Editing of Plants. In: Langel, Ü. (eds) Cell Penetrating Peptides. Methods in Molecular Biology, vol 2383. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1752-6_39

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  • DOI: https://doi.org/10.1007/978-1-0716-1752-6_39

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1751-9

  • Online ISBN: 978-1-0716-1752-6

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