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Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples

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Book cover Mycobacterium ulcerans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2387))

Abstract

Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.

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Author information

Authors and Affiliations

  1. Biology and Genetics of Bacterial Cell Wall, Institut Pasteur, Paris, France

    Aline Rifflet

  2. INSERM, Equipe Avenir, Paris, France

    Aline Rifflet

  3. Immunobiology of Infection Unit, Institut Pasteur, INSERM U1221, Paris, France

    Caroline Demangel & Laure Guenin-Macé

Authors
  1. Aline Rifflet
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  2. Caroline Demangel
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  3. Laure Guenin-Macé
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Corresponding author

Correspondence to Laure Guenin-Macé .

Editor information

Editors and Affiliations

  1. Swiss Tropical and Public Health Institute, Basel, Switzerland

    Gerd Pluschke

  2. Stanford School of Medicine Department of Pathology, Stanford University, Stanford, CA, USA

    Katharina Röltgen

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Rifflet, A., Demangel, C., Guenin-Macé, L. (2022). Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples. In: Pluschke, G., Röltgen, K. (eds) Mycobacterium ulcerans. Methods in Molecular Biology, vol 2387. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1779-3_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1779-3_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1778-6

  • Online ISBN: 978-1-0716-1779-3

  • eBook Packages: Springer Protocols

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